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Methods ①anti-inflammatory trial: 40 rats were randomly divided into control group, high-dose bushenlizhuo capsule group, middle-dose bushenlizhuo capsule group and low-dose bushenlizhuo capsule group(10 rats in each group).the rats in control group were given distilled water, and the rats in experimental groups were given different doses of capsule(4.0、2.0、1.0g/kg), then enhance the capillary permeability of abdominal skin of rats by using xylic acid,and the od numerical value was detected.

(1)抗炎实验:将40只小鼠随机分为补肾利浊胶囊高、中、低剂量组及对照组各10只,补肾利浊胶囊高、中、低剂量组分别给予相应剂量的补肾利浊胶囊(分别为4.0、2.0、1.0 g/kg),对照组给予蒸馏水,采用二甲苯酸致小鼠腹部皮肤毛细血管通透性增高,测定光密度值;(2)镇痛实验:40只小鼠分组及给药同抗炎实验,采用腹腔注射醋酸致小鼠出现扭体反应,纪录扭体次数并计算镇痛百分率;(3)抗前列腺炎实验:将雄性大鼠50只随机分为补肾利浊胶囊高、中、低剂量组及模型对照组及正常对照组,各10只。

Ginger oil was besmeared on the skin of mice and then the mice were either directly infected with Schistosoma japonicum cercariae or with the Schistosoma japonicum cercariae after washed by muddy water.

方法将实验小鼠随机分为A、B、C 3组,先将生姜油涂抹于A、B组小鼠皮肤, A组小鼠直接用血吸虫尾蚴感染,B组小鼠用泥浆水洗擦后感染血吸虫尾蚴,C组为对照组,A、B两组结果与对照组比较,观察生姜油预防血吸虫尾蚴感染的效果。

Methods Forty mice were randomly divided into 4 groups: one normal control group,mice in the other three groups were all infected each with 25 cercariae, the infected control group was fed for 10 weeks after infection, and 2 weeks after infection, the high dose PTX group was given PTX 360 mg/ for 8 weeks and the low dose PTX group was given PTX 180 mg/also for 8 weeks.

将40只小鼠随机分为4组,其中3组每只小鼠人工感染日本血吸虫尾蚴25条,1组感染后继续喂养10周,不作治疗,作为感染对照组;2组分别于感染后2周用PTX 360 mg/和180 mg/灌胃治疗8周;另1组不感染、也不接受药物治疗,与上述3组同步喂养10周,作为正常对照组。10周后将上述4组小鼠分别剖杀取肝组织,光镜观察肝组织病变;用免疫组化染色方法检测小鼠肝组织中Bcl-2、Bax的水平。

The KM reduced mice' s spraining body number of times caused by acetic acid obviously, prolonged the latent period of their spraining body notably;evidently extended latent period of stimulation by hot board and improved their pain threshold value.

川乌能显著减少醋酸所致小鼠扭体次数,延长小鼠扭体潜伏期:明显延长热板刺激小鼠添足潜伏期,提高热板小鼠痛阈值。

The result showed the egg protein could significantly prolong swimming time, increased the reserves of liver glycogen, and decreased the content of blood lactic acid, which indicated the egg protein of Rana dybowskii has anti-fatigue function.

结果表明,东北林蛙卵蛋白对小鼠体重增长无显著影响,可降低运动后小鼠血乳酸含量,增加小鼠负重游泳时间及肝糖原含量。研究显示,东北林蛙卵蛋白具有增加小鼠抗疲劳的活性作用。

ResultsThe Ziziphus jujube leaf extract could inhibit autonomic activities of the mice, prolong the sleep time induced by pentobarbital sodium, enhance the rate of falling asleep induced by barbital sodium and inhibit the central stimulating effects induced by amphetamine.

结果酸枣叶提取物可对小鼠自主活动呈明显抑制作用,延长戊巴比妥钠诱导的小鼠睡眠时间,增加巴比妥钠诱导的小鼠睡眠发生率和缩短巴比妥钠诱导的小鼠睡眠潜伏期。

MethodsAnti-inflammation effect of wild and cultivated Polygonun capitatum were observed by mouse abdominal cavity capillary permeability increase test induced by acetic acid, mouse auricle swelling test induced by xylene, mouse pedal swelling test induced by carrageenin and cotton granuloma test in mouse.

方法采用醋酸致小鼠腹腔毛细血管通透性亢进、二甲苯致小鼠耳肿胀实验、角叉菜胶致小鼠足肿胀和小鼠棉球肉芽肿等实验进行抗炎实验,采用醋酸扭体法进行镇痛实验。

The LD50 of acute toxicity to mice and chronic accumulated toxicity to bandicoots of crude and processed Radix Aristolochice were observated.Intestinal and myokinetic influence of normal and revulsive hyperactive gastrointestinal motility of mice induced by neostigmine were observated by giving aqueous extract and alcoholic extract of crude and processed Radix Aristolochice.Relieving pain and eliminating inflammation to mice also were observated .

采用小鼠急性毒性和大鼠蓄积性毒性方法观察青木香炮制前后对小鼠急性毒性LD50和对大鼠的慢性蓄积性毒性作用;观察了青木香炮制前后的水煎剂和醇提物对正常和新斯的明诱导的胃肠运动机能亢进小鼠肠肌运动的影响;并观察了对小鼠的镇痛和抗炎作用。

The concents of testosterone and NO of the mouses gavaged with high dosages of Chinese chive extractiong were not significantly more than that of giving middle dosages.This indicated that Chinese chives might be more safe than traditional chinese herbals of sexual improvement function.2、The screening of sexual improvement function chemical substances of Chinese chivesThe crude extract was leached to four phases by petroleum ether, ethyl acetate,n-butanol and water.By doing animal tests to detect the contents of testosterone and NO in serum of mices,the result was showed that the extracts of petroleum ether and n-butanol have sexual improvement function,especial the n-butanol phase.

小鼠灌喂不同剂量的韭菜提取液试验结果来(来源:ABCa539a5论文网www.abclunwen.com)看,灌胃高剂量的小鼠的睾酮含量和NO含量相对于中剂量来说并不上升,而是保持稳定,因为睾酮含量和NO含量过度上升可能会带来付作用,所以由此可以说明其食用安全性高,副作用小。2、韭菜改善性功能物质的筛选将韭菜粗提物依次通过不同溶剂萃取,分成石油醚、乙酸乙酯、正丁醇和水四个萃取相,通过小鼠灌胃的动物试验,以小鼠血液NO含量和睾酮含量为指标,发现正丁醇和石油醚萃取相中的物质有改善性功能的效果,尤其是正丁醇相效果非常显著。

The sepsis model was established by Cecal ligation and puncture in mice. Thirty male BALB/c mice were randomly divided into five groups: sham operation group, the twelfth hour, twenty-forth hour, thirty-sixth and forty-eighth hour group after CLP.

采用盲肠结扎穿孔术建立脓毒症BALB/c小鼠模型。30只小鼠随机分为5组,分别为假手术组,CLP后12、24、36、48 h组,其中假手术组小鼠在假手术后24 h取标本检测作为对照组,其余四组小鼠分别在CLP术后的12 h、24 h、36 h和48 h取标本待检。

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