小鼠

Methods: Models of leucocytopenia induced by irradiation were prepared by 60Co -ray.

采用~(60)Co照射小鼠，造成小鼠白细胞减少模型，即产生小鼠血虚证。评价SMI对于~(60)co所致的小鼠血虚证的治疗作用。

SMI had protection and therapeutic effects on leucocytopenia of mice induced by irradiation.

试验还显示，照射后四天SMI大、中剂量组小鼠骨髓有核细胞数与正常对照组无显著性差异，说明其骨髓造血功能比较活跃，提示SMI对放射性损伤所致小鼠血虚证有一定的保护和治疗作用，对于小鼠白细胞减少有较好的治疗效果。结论：SMI对小鼠放射性损伤所致血虚证有一定的保护和治疗作用。

Methods: Eighty mice were randomly divided into high, middle, and low dosage groups which were orally given 1.5 g/kg, 0.75 g/kg, 0. 375 g/kg of ant powder respectively, twice a day for 10 days. A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组，黑蚂蚁粉给药量按小鼠体重1.5g/kg、0.75g/kg、0.375g/kg计算，连续10d灌胃给药后，测定各组小鼠的体重、胸腺和脾脏重量，计算胸脾指数；检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组，黑蚂蚁粉给药量按小鼠体重1.5 g/kg、0.75 g/kg、0.375 g/kg计算，连续10 d灌胃给药后，测定各组小鼠的体重、胸腺和脾脏重量，计算胸脾指数；检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

Objective To obtain the basic data of seroenzyme activity of p21(superscript HBsAg/HbsAg) and p21(superscript HBsAg/HbsAg) transgenic mice on the different age, and To study the infection of the seroenzyme activity, of the p21(superscript HBsAg/HbsAg) and p21(superscript HBX/HBX) was introduced into p21 locus of the mouse.

目的 测定p21(上标 HBsAg/HBsAg)和p21(上标 HBsAg/HBsAg)转基因小鼠纯合型及野生型小鼠的血清酶，探讨各种血清酶在两种纯合型小鼠及野生型小鼠的变化规律。

We discuss the effects of GLJ about depressing the spasmodic ileum by experiments on isolated apparatus or holistic animal, in order to learn the mechanism and offer the data to exploiting and researching the new preparation.1 The action of GLJ on the intestinal smooth muscle of holistic animal.1.1 Observe the action of GLJ on normal intestinal propulsion of NIH rats and sthenic intestinal propulsion due to Neostigmine.

GLJ对在体动物肠平滑肌运动功能的影响 1.1观察GLJ对正常小鼠肠推进功能和对新斯的明所致推进功能亢进的小鼠的肠推进功能的影响，结果表明，50mg／kg、100mg／kg、200mg／kg的GLJ对正常小鼠肠推进功能均无显著影响，但对新斯的明所致推进亢进小鼠肠推进功能均有显著抑制作用。

Methods: The mice for experiment were immunizied with subcutaneous injection of type Ⅱ collagen to induce arthritis after ovariectomy. Severity of joint swelling, radioimmunoassay of serum estradiol (E2),osteocalcin and tumor necrosis factor, and pathological changes of joint, including changes on synovia, articular cartilage and bone, were observed once weekly.

切除小鼠卵巢后，Ⅱ型胶原皮下注射以诱导关节炎；每周1次观察小鼠的关节肿胀程度；组织切片光镜下观察小鼠滑膜、关节软骨及骨的改变；放射免疫法检测小鼠血清雌二醇、骨钙素、肿瘤坏死因子的变化。

objective to investigate the existence of the erythropoiesis inhibitors.methods the present study included twelve undialyzed esrd patients with anemia.the sera from the uremic patients were added to cfu-e and bfu-e culture in the concentrations ranging from 1.25% to 5%.in vitro cfu-e and bfu-e growth in the presence of sera from esrd patients was compared with in the presence normal human subjects with the use of normal mice bone marrows.results the effects of the sera from uremic patients on cfu-e and bfu-e colony growth in concentrations ranging from 1.25% to 5% were investigated to be dose-dependent.the effect increased with the concentrations increasing of the sera（p＜0.05）.there were not significant inhibitory effect on the cfu-e and bfu-e growth of mice bone marrow in vitro from sera of the normal subjects.conclusion the erythropoiesis inhibitors exist in uremic sera which has a dose-dependent inhibitive effects on the growth cfu-e and bfu-e of normal mice bone marrow cells.

选择12例尿毒症肾性贫血未透析患者为试验组，另选正常健康体检者12例为正常对照组，将实验组、正常对照组血清分别稀释成不同终浓度，采用甲基纤维素细胞培养的方法对小鼠骨髓细胞进行培养，对比不同浓度血清组红系集落形成单位、红系爆式形成单位的集落数。结果在实验组中，与血清浓度为0的浓度组相比，与各浓度组血清共同培养的小鼠骨髓cfu-e和bfu-e的集落数均明显减少，且随血清浓度的增加，小鼠骨髓cfu-e和bfu-e的集落数逐渐减少，各浓度组间差异有显著性（p＜0.05）。结论尿毒症患者血清抑制小鼠骨髓红系祖细胞集落形成，随患者血清浓度的增加，抑制作用增强，具有剂量依赖性。

The rats in control group were given distilled water, and the rats in experimental groups were given different doses of capsule（4.0、2.0、1.0g/kg）, then enhance the capillary permeability of abdominal skin of rats by using xylic acid,and the OD numerical value was detected.

(1)抗炎实验：将40只小鼠随机分为补肾利浊胶囊高、中、低剂量组及对照组各10只，补肾利浊胶囊高、中、低剂量组分别给予相应剂量的补肾利浊胶囊(分别为4.0、2.0、1.0 g/kg)，对照组给予蒸馏水，采用二甲苯酸致小鼠腹部皮肤毛细血管通透性增高，测定光密度值；(2)镇痛实验：40只小鼠分组及给药同抗炎实验，采用腹腔注射醋酸致小鼠出现扭体反应，纪录扭体次数并计算镇痛百分率；(3)抗前列腺炎实验：将雄性大鼠50只随机分为补肾利浊胶囊高、中、低剂量组及模型对照组及正常对照组，各10只。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。