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The results indicated that nutrition element stored in bulblet was consumed for new leaves and stem growing before plantlet stage.

结果表明,百合小鳞茎在苗期以前,主要以消耗鳞茎中的养分为主;苗期后对营养元素的吸收量逐渐增大。

The differentiation ability for different parts of bulblet leaves was studied,and the results showed that the apical part of leaves did not regenerate,however,mass shoot regeneration occurred from the leaf base,occasionally from the middle part.

通过对再生苗叶片不同部位的再分化实验,比较得出小鳞茎叶的叶片基部分化能力非常强,而叶片上部基本不分化,鳞茎叶基部是建立高频快速再生体系的首选材料。

The characteristic of nutrient absorption and distribution in lily (Longiflrorum and Asiatic hybrids 'Evening Star') were investigated during the bulblet swelling development, the circumference of which is 4~6cm.

本文以周径为4~6cm的'耀眼'百合的小鳞茎为试材,研究了常规栽培条件下百合小鳞茎膨大发育过程中养分的吸收和分配规律。

The results showed that NAA at low concentrations 0.1~0.2 mgL^(-1 was more beneficial for root induction, the rate of rooting was up to 100%. 1/4MS was more suitable to deal with the plantlet rooting and form small bulb. The optimal ratio of IBA and AC for rooting was 2.0 mgL^(-1) IBA+0.1%AC. The in vitro growth of plantlets were the strongest, and survival rate was 100% after incubating at 4℃ for 4 weeks when the diameter of bulblet was ≥9 mm.

结果显示;低浓度的NAA0.1~02mgL^(-1有利于根的诱导;生根率均达100%;大量元素1/4MS处理更适合试管苗的生根和小鳞茎形成;"IBA+AC"处理最佳组合是2.0mgL^(-1) IBA+0.1%AC;鳞茎直径≥9mm的试管苗去顶4℃低温冷藏4周;移栽后喷施1/2MS营养液;长势最好;成活率达到100%。

Two specieses, Lycoris radiate and Lycoris aurea, were selected for the studying of artificial propagation in vitro. A few specieses of Lycoris, from the southeast area of China and part of locations in Japan, were chosen to research their karyotype differentiation and to measure their genetic diversity by ISSR analysis. The results could be summarized as follows.1. The vegetative propagation conditions of Lycoris aurea and L. radiate in vitro was studied by two-scaling. Different illuminate condition had an effect on the bulblet formation rate. To the species of L. radiate, the rate was higher in the state of 16h 800-12001x illumination than in the darkness. The medium also affected the new bulblet formating rate. When the culture was MS medium 0.2 mg/L NAA 4 mg/L 6-BA, the bulblet formationg rate of L. aurea was 220%;at the same time, when the medium was MS 0.2 mg/L NAA 2 mg/L 6-BA, the rate of L.

本研究选择西南部分地区及日本的几个石蒜品种,从细胞学和DNA分子角度,分析了它们的遗传多样性水平和遗传结构状况,对红花石蒜和忽地笑的离体繁殖技术进行了初步研究,得出如下结论: 1 进行了红花石蒜和黄花石蒜双鳞片快速繁殖条件的研究,结果表明:红花石蒜在16小时800-1200 lx光照下比黑暗条件下出芽率要高;黄花石蒜在MS NAA0.2mg/L 6-BA4mg/L下出芽率为220%,红花石蒜在MS NAA0.2mg/L 6-BA2mg/L下出芽率为108%;NAA比IBA有利于石蒜生根;硅藻土显著提高黄花石蒜双鳞片出芽率,活性炭起抑制作用;6%蔗糖浓度有利于红花石蒜小鳞茎增重,MS 6-BA4mg/L NAA0.5mg/L培养基有利于小鳞茎增殖,切割一刀比两刀有利于小鳞茎增殖。

Rapid propagation of Lilium lancifolium Thunb. was introduced in five aspects: the reproduction of bulblets,the extantion of bulblets, the redifferentiation of bulblets through callus, the environment factors on bulblets propagation. In addition,the valuable components were analogized.

本文从小鳞茎的增殖、小鳞茎的膨大、愈伤组织诱导再生小鳞茎、环境因子对小鳞茎生长的影响四个方面探索了组织培养快速繁殖宜兴百合的方法,大大提高了宜兴百合的生长量;并通过组培百合与栽培百合进行有效成分的对比分析,以此保证组培百合的品质。

The small bulbs induced were full and the shapes of them were regular. The explants incubating in other seasons were not suitable. By comparison different incision methods, we found it' was better taking stem plate carrying bulb leaves as explants than any other treatments.

通过对不同季节的水仙鳞茎外植体进行组培观察,发现8月份至次年5月份接种的水仙外植体,小鳞茎的诱导率几乎达到100%;并且,小鳞茎比较饱满,形态比较规则,其它时间接种的水仙外植体的组培效果均不理想。

In order to breed new germplasm for Narcissus tazetta,the bulb of Narcissus tazetta is used as explants.The adventitious buds is induced in MS media with hormone combination.γ-ray irradiation treatment of Narcissus tazetta combined with tissue culture is studied.Three materials,including the bulb basal plate tissues inoculated for 7 days,tissues transferred in fresh differentiation culture medium for three days after inoculated for 25 days,and small bulbs in vitro,are studied.

以带鳞片叶的鳞茎盘为外植体,接种在添加激素的MS培养基上,通过激素组合筛选合适的培养基获得不定芽,同时对在预分化培养基上接种7D的外植体、接种25D后转接到分化培养基上3D的外植体、诱导产生的试管小鳞茎进行辐照诱变处理,研究了60COΓ射线对中国水仙丛生芽分化以及小鳞茎生长的影响。

In cider to breed new gern1asm for Narcissus tazetta, the bulb of Narcissus tazetta is used as explants. The adventitious buds is induced in MS media with hormone combination.γ-ray irradiation treatment of Narcissus tazetta combined with tissue culture is studied. Three materials, including the bulb basal plate tissues inoculated for 7 days, tissues transferred in fresh differentiation culture medium for three days after inoculated for 25 days, and small bulbs in tarn, are studied.

以带鳞片叶的鳞茎盘为外植体,接种在添加激素的MS培养基上,通过激素组合筛选合适的培养基获得不定芽,同时对在预分化培养基上接种7d的外植体、接种25d后转接到分化培养基上3d的外植体、诱导产生的试管小鳞茎进行辐照诱变处理,研究了60Coγ射线对中国水仙丛生芽分化以及小鳞茎生长的影响。

The bulb scales had a higher induction rate of 89.3% to 93.3%, better quality and lower decay rate and the best rooting, in which single-scale reproduction coefficient reached 1.14 to 1.19 when cutting in soil from original environment and peat and pearlite (1:1) as medium under the condition of 25℃ and dark, followed by cutting in peat and sand (2:1) and peat and vermiculite and perlite (1:1:1). There were the highest single-scale reproduction coefficient (1.38) when cutting in sand, but the induction rate was lowest (58.7%). The bulb scales was easy to rot and unfit for use in peat.

以原生土、草炭+珍珠岩(1:1)这2种基质最好,诱导率达89.3%93.3%,平均每鳞片繁殖小鳞茎数较高,达1.14~1.19个,产生的小鳞茎质量也较好,而且鳞片腐烂率低,生根效果也最好;草炭+河沙(2:1)及草炭+珍珠岩+蛭石(1:1:1)的扦插效果仅次于草炭+珍珠岩(1:1);河沙扦插鳞片时,平均每鳞片繁殖小鳞茎数最高,达1.38个,但诱导率最低(58.7%),易造成鳞片干萎,草炭则易造成鳞片大量腐烂,均不宜使用。

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