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The microsporogenesis and development of male gametophyte in Tsoongiodendron odorum Chun were observed by using the paraffin section method.

采用石蜡切片法对观光木的小孢子发生和雄配子体发育进行了解剖学研究。

Monosomic 3, monosomic 5, monosomic 10, trisomic 4 and trisomic 5 as experiment materials, their meiosis behavior of pollen mother cell, pollen characteristics, physiological and biochemical characteristics of anther, section observation of pollen development and the structure of embryo sac were studied, and the effects on the seed set and transmission rate were analyzed.

本文以大白菜同源四倍体小孢子培养产生的且已鉴定为3号单体、5号单体、10号单体、4号初级三体、5号初级三体的非整倍体株系及二倍体株系等为试材,对各材料的结籽率、雄配子传递率进行了统计,并对花粉母细胞减数分裂行为、花粉特性、花粉发育和胚囊结构以及花药生理生化特性等方面进行了观察研究,进而分析了其对大白菜单体n-1雄配子和三体n+1雄配子传递率的影响。

For the approach to the relations between the endangered situation and their sexual reproductive processes in Cimicifuga nanchuanensis, some comparative studies were made in C.simplex , a close and widespread species.

为了探讨南川升麻濒危与其生殖过程的联系。对广布近缘种单穗升麻进行了部分对照研究。论述单穗升麻的小孢子发生和雄配子体发育以及花药壁的发育。

The temperature affects the sustained time of secondary sporogenous cell split, the sustained time of microspore mother cell, formation speed and regular of male gametophyte.

温度影响次生造孢细胞持续分裂的时间、小孢子母细胞的持续时间及雄配子体的形成速度和整齐度。

The results showed: four devices flower pollen sacs, anther wall conforms to the basic, from epidermis, the indoor wall, middle (2-3 layers) and tapetal composition tapetum to glandular type.

对悬铃木小孢子发生及雄配子体发育过程进行了观察,结果表明:花药具4个花粉囊,花药壁发育为基本型,由表皮、药室内壁、中层(2-3层)和绒毡层组成,绒毡层为腺质型。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究,我们提出了初步的NodD操纵子激活模型:第一,四聚体是NodD蛋白的功能单位,它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子;第二,小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体,在我们的模型中,NodD四聚体缩小其V形主体的弯折角,进而缩短其DNA结合功能域的间距;第三,小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用,NodD的构象改变由蛋白质经其双铆钉位点传递给DNA;第四,只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物;第五,不排除NodD与RNA聚合酶可能有直接的相互接触位点,不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物,但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

A two directional SSHcDNA library of cold acclimation about Physcomitrella patens gametophyte was constructed.

本实验构建了一个小立碗藓配子体冷胁迫的SSHcDNA双向文库。

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