小菜蛾

In addition this paper,"Bio-Rad method," two parasitic wasps of the post-embryonic development the process of protein accumulation in young bees and the host Plutella xylostella in this process in vivo haemolymph protein content were measured and found that over time, was gifuensis parasitic larvae of the diamondback moth haemolymph protein content showed an upward trend in nearly a straight line, Ichneumonidae parasitic For example, in the early parasitic diamondback moth haemolymph protein content also showed the trend of slow growth, but in the mid-and late parasitic young bee about to be completed in the development of the host body when to stop feeding, a significant downward trend却呈, and significantly lower than the third day of its own protein content; on the same day of the different species of the diamondback moth parasitic wasp hemolymph protein content compared Ichneumonidae Parasitism of diamondback moth have hemolymph protein content than in the third and fourth day after the parasitic Braconidae hemolymph content to be high, but very different in the case of the fifth day, as evidenced by her side after the parasitic bee moth haemolymph protein content is cocoon of the parasitic bee hemolymph protein content of 0.7 times.

另外本文采用"Bio-Rad法"对两种寄生蜂胚后发育过程中幼蜂蛋白积累及寄主小菜蛾在此过程中体内血淋巴蛋白含量进行了测定，结果发现随时间的推移，被茧蜂寄生小菜蛾幼虫的血淋巴蛋白含量是呈近直线增加趋势，而对于姬蜂寄生来说，在寄生初期小菜蛾血淋巴蛋白含量也呈缓慢增长趋势，但在寄生中后期，幼蜂即将完成在寄主体内的发育取食停止时，却呈大幅下降趋势，且明显低于其自身第三天的蛋白含量；对同一天的被不同种寄生蜂寄生的小菜蛾血淋巴蛋白含量进行比较，姬蜂寄生过的小菜蛾血淋巴蛋白含量在第三天和第四天均比茧蜂寄生后的血淋巴含量要高，但在第五天的情况大不相同，表现为被姬蜂寄生后的小菜蛾血淋巴蛋白含量是被茧蜂寄生的血淋巴蛋白含量的0.7倍。

Result In the first 4 d of experiment, the corrected mortalities of spore suspensions with various concn. of P. cicadae APC20 to P. xylostella were lower than 50%, but on the 7 th day, that of spore suspension with concn. of 3.0×10^8 spores/ml reached 85.11% and the insect corpses was bestrewed in the mycelium and spore powder of P. cicadae, indicating that APC20 showed stronger parasitic lethality to P.

结果］药后第1~4天，蝉拟青霉APC20各个浓度的孢子悬浮液对小菜蛾的校正死亡率均低于50%，而第7天，浓度为3.0×10^8个／ml的孢子悬浮液对小菜蛾的校正死亡率达85.11%，并且虫尸布满蝉拟青霉的菌丝体和孢子粉，说明APC20对小菜蛾呈现较强的寄生致死性。

Xylostella. P. cicadae APC20 had stronger infecting potential to P. xylostella and the LC50 on the 4th~7th day of treating P.

蝉拟青霉菌株APC20对小菜蛾具有较强的侵染力，处理小菜蛾第4~7天的LC50分别为5.3×10^5、1.1×10^5、1.1×10^4、8.9×10^2个／ml。

Effects of test extracts on oviposition deterrent of the diamondback moth were studied.

所选材料容易获得，对小菜蛾忌避作用明显，在庭园小菜蛾防治中具有潜在的应用价值。

Host plants affected the survival, development time and reproduction of P.xylostella and indirectly affected the survival, development time, fecundity and sex ratio of the parasitoid.

6小菜蛾及菜蛾绒茧蜂的生存、发育、繁殖等均不同程度地受寄主植物的影响，小菜蛾成虫期的产卵嗜好性并不反映植物对其幼虫期的相对适合度。

Plutella xylostella larvae of a strain highly resistant to spinosad were used as hosts to rear the parasitoid Cotesia plutellae. When the parasitoids had developed to the stage of early instar larva, the host larvae were treated with 50 mg/L spinosad on the terga by topical application and then fed with cabbage leaves treated with 50 mg/L spinosad to allow the host larvae and the parasitoid larvae to develop. Three days after treatment, the parasitoid larvae inside the host were collected by dissecting the host larvae. The body fluid of P. xylostella and the bodies of parasitoid larvae were then analyzed with HPLC. Both of the active components, spinosyn A and spinosyn D, of spinosad were detected in the body fluid of host larvae and in the bodies of parasitoid larvae, and the residue concentrations were 2.79 mg/L and 0.94 mg/L in the host and the parasitoid respectively.

以对多杀菌素高抗的小菜蛾Plutella xylostella幼虫作为菜蛾绒茧蜂Cotesia plutellae的寄主，待绒茧蜂发育到1龄幼虫时，将浓度为50 mg/L的多杀菌素点滴到寄主幼虫背板上，随后让寄主幼虫取食经50 mg/L多杀菌素处理过的甘蓝叶片，寄主幼虫和其体内的蜂幼虫再发育3天后，将寄主幼虫解剖取出蜂幼虫，用高效液相色谱法对经多杀菌素处理的小菜蛾幼虫体液以及绒茧蜂幼虫匀浆液进行检测，结果多杀菌素的2个活性成分spinosyn A和spinosyn D均被检测到，两者的多杀菌素残留浓度分别是2.79 mg/L和0.94 mg/L。

Xylostella. The synergism ability with dipping method l4as more obvious than with contact and stomach method. Among the six adjuvants, copper abietate, 1427 and 412103 had 3.71, 2.82 and 2.72-fold synergism ratio, respectively.

结果表明：在400mg/L以下，各阳离子助剂单独使用对小菜蛾3龄幼虫均无生物活性，但可明显提高阿维菌素对小菜蛾的药效。

In addition this paper,"Bio-Rad method," two parasitic wasps of the post-embryonic development the process of protein accumulation in young bees and the host Plutella xylostella in this process in vivo haemolymph protein content were measured and found that over time, was gifuensis parasitic larvae of the diamondback moth haemolymph protein content showed an upward trend in nearly a straight line, Ichneumonidae parasitic For example, in the early parasitic diamondback moth haemolymph protein content also showed the trend of slow growth, but in the mid-and late parasitic young bee about to be completed in the development of the host body when to stop feeding, a significant downward trend却呈, and significantly lower than the third day of its own protein content; on the same day of the different species of the diamondback moth parasitic wasp hemolymph protein content compared Ichneumonidae Parasitism of diamondback moth have hemolymph protein content than in the third and fourth day after the parasitic Braconidae hemolymph content to be high, but very different in the case of the fifth day, as evidenced by her side after the parasitic bee moth haemolymph protein content is cocoon of the parasitic bee hemolymph protein content of 0.7 times.

另外本文采用&Bio-Rad法&对两种寄生蜂胚后发育过程中幼蜂蛋白积累及寄主小菜蛾在此过程中体内血淋巴蛋白含量进行了测定，结果发现随时间的推移，被茧蜂寄生小菜蛾幼虫的血淋巴蛋白含量是呈近直线增加趋势，而对于姬蜂寄生来说，在寄生初期小菜蛾血淋巴蛋白含量也呈缓慢增长趋势，但在寄生中后期，幼蜂即将完成在寄主体内的发育取食停止时，却呈大幅下降趋势，且明显低于其自身第三天的蛋白含量；对同一天的被不同种寄生蜂寄生的小菜蛾血淋巴蛋白含量进行比较，姬蜂寄生过的小菜蛾血淋巴蛋白含量在第三天和第四天均比茧蜂寄生后的血淋巴含量要高，但在第五天的情况大不相同，表现为被姬蜂寄生后的小菜蛾血淋巴蛋白含量是被茧蜂寄生的血淋巴蛋白含量的0.7倍。

In this paper, an important pest of cruciferous vegetables xylostella and its two dominant species in the parasitic wasp - Cotesia plutellae bee, Diadegma semi-closed two parasitic system studied two parasitic wasps of Nutritional regulation of the host Plutella xylostella, from parasitic to parasitic found after the fifth day, five days, all groups the body weight of diamondback moth larvae are showing an increasing trend, not parasitic larvae of the diamondback moth has been higher than was body weight parasitic larvae of Plutella xylostella, and was Ichneumonidae parasitic larvae of the diamondback moth important than being clear Braconidae parasitic weight of the diamondback moth larvae.

本文以重要的十字花科蔬菜害虫小菜蛾及其两种优势种内寄生蜂——菜蛾盘绒茧蜂、半闭弯尾姬蜂两个寄生体系为研究对象，研究了两种寄生蜂对寄主小菜蛾营养的调控，发现从寄生至寄生后的第五天，五天时间内，所有组小菜蛾幼虫的体重均是呈现增加的趋势，未被寄生的小菜蛾幼虫体重一直保持大于被寄生小菜蛾幼虫，而且被姬蜂寄生的小菜蛾幼虫体重要明显的大于被茧蜂寄生的小菜蛾幼虫体重。

After induced with IPTG, the proteins expressed by different fragments were analyzed by SDS-PAGE, and the results showed that all these truncated proteins could be expressed in E. coli BL21. Bioassay result against Plutella xylotella showed that the active fragment of Cry1Ba3 protein was located between amino acid No. 22 and No. 655. As the insecticidal activity of the truncated protein containing amino acid No. 54-655 was declined significantly, and No. 22-627 and No.

采用浸叶法检测这些蛋白片段对小菜蛾的杀虫活性，研究结果表明含有第1～685位和含有第22～655位氨基酸的蛋白片段对小菜蛾的毒性，与全长Cry1Ba3蛋白相比，没有改变；含有第54～655位氨基酸的蛋白片段对小菜蛾的毒力明显降低；而含有第22～627位和含有第85～655位氨基酸的蛋白片段完全丧失了对小菜蛾的活性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失