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The distributions of Hup A into rat brain tissues following intranasal dosing were compared with those after intravenous and intragastric dosing by tissue homogeneization.

结果大鼠鼻腔给药血浆AUC0→6h为静注的0.94倍,但脑脊液AUC0→6h为静注和灌胃的1.3和2.3倍;大鼠鼻腔给药后大脑、海马、小脑、左右嗅球的AUC0→6h分别为静注的1.5,1.3,1.0,1.2和1.0倍,为灌胃的2.7,2.2,1.9,3.1和2.6倍。

Objective:To examine the effects of acute or chronic administration of 7-nitro indazole (7-NI) on the MAC of sevoflurane and cerebellar cGMP levels in mice.

目的:应用神经型NO合成酶抑制剂7-硝基吲唑(7-NI),观察急、慢性用药对七氟醚 MAC及小鼠小脑cGMP的影响。

In the brain stem,there were very dense orexin B immunoreactive fibers in the superior colliculus,inferior colliculus,interpeduncular nucleus,dorsal raphe nucleus and central gray.

此外,在小脑、脊髓和垂体柄及垂体后叶也有少量的阳性纤维的分布。

Because of entirely intracerebellar growth, meningeal origin is also unlikely.

由于肿瘤完全在小脑内生长,所以也不太可能是脑膜起源的肿瘤。

We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此,本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs,使用无血清培养基进行扩增、培养,用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定,对少突胶质系细胞表达部分营养因子的情况进行检测;采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测;将OPCs移植入成年SD大鼠玻璃体内,利用上丘逆行荧光标记技术,观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间;将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内,观察不同时期视网膜内髓鞘形成与分布特点,分析髓鞘的超微结构,并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

A 30 min exposure to peroxynitrite (10〓μmol/L) followed by incubation in fresh medium induced apoptosis in cultured cerebellar granule cells, which was confirmed by DNA laddering, chromatin condensation, nuclei fragmentation, and the formation of apoptotic bodies.

原代小脑颗粒细胞经10μmol/L过氧化亚硝基处理30min,并继续培养一定时间后逐渐发生细胞凋亡,主要表现为DNA链断裂、染色质凝缩、核破裂、形成凋亡小体。

Lipomatous tumors in internal auditory canal and cerebellopontine angle are very rare; low-grade papillary adenocarcinoma of endolymphatic sac origin is rarely reported and has low malignant potential.

脂肪瘤样迷离瘤是一种发生在内耳听神经管或桥小脑角非常罕见的由脂肪、纤维、平滑肌、横纹肌以及神经成分组成的良性肿瘤。内耳内淋巴囊来源的低级别乳头状腺癌是发生在内耳内淋巴囊的特别罕见的低级别肿瘤。

Moter, sensory and cerebellar functions were normal in the upper extremities.

运动、感觉和小脑功能在上肢都正常

Results:There were 12 cases of peripheral sensory neuropathy,12 cases of myasthenic syndrome,6 cases of spinal cord compression,4 cases of brain stem encephalitis,3 cases of subacute cerebellar degeneration.3 cases were diagnosed in 1 week after appearing neurological symptoms,and 3 cases were diagnosed in 1 month,the other 16 cases were diagnosed after many months or years while the neuropathy was existing.

结果:癌性感觉性周围神经病12例,肌无力综合征8例,脊髓压迫症6例,脑干脑炎4例,亚急性小脑变性3例。3例在出现神经系统症状后1周内确诊,3例在1月内确诊,其余16例均在神经病变持续存在数月至数年后经多次检查确诊为肿瘤。

Nanocephalic dwarfs differ from other dwarfs in that they preserve the skeletal proportions of normal adults.

小脑侏儒不同於其它的侏儒的地方是他们仍存有正常成人的骨骼比例。

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

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