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The nanoplex were smaller than the unmodified nHAP; thelipiodol/nanoplex emulsion, with the mean diameter at 75±11.8nm can disperse moreuniformly and be more soluble and stable, contrary to the unmodified nHAP and nanoplex Conclusion: Nanoplex/pDNA mass ratios greater than 15 had exhibited completepDNA absorption onto positive charged polyplex and pDNA protection from thedestruction of nucleinase in rabbit serum. Unmodified nHAP may can\'t induce effectivegene delivery.PartⅡThe construction of the recombint eukaryotic expressive vector ofwild-type human p53 and RbObjective: To construct the recombint eukaryotic expressive vector of wild-type humanp53 and Rb by utilizing the theory of molecular medicine.

结果nHAP颗粒本身大小小于100nm,但由于颗粒表面本身带负电,不能包括有效地结合及保护DNA;经Ca~(2+)修饰后,颗粒可带正电,可一定程度地结合和保护DNA,但聚合物本身的中性电与带负电的细胞膜结合,而且本身的颗粒则变地容易聚集而成为很大的颗粒,也不利于细胞的吞噬;经PLL修饰后,颗粒溶解性提高,直径变小,表面带正电,电泳结果显示PLL-nHAP颗粒可包括有效地结合和保护DNA免受兔血清的破坏,而且复合物表面的正电也包括有利于与细胞膜的结合与吞噬。

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