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Allen was a researcher at the Department of Neurobiology, The Babraham Institute, Cambridge, UK. Dr. Allen's research skills and techniques include DNA/RNA manipulation and purification/isolation, electrophoreses, PCR, cloning, all blottings, library screening, genetic mapping, design and construction of transgenes, artificial chromosome engineering, design and construction of vectors for gene targeting in ES cells, cell culture, histological techniques, mouse behavioural analysis, small animal surgery, and bioinformatic tools such as sequence databases and analysis tools.

从1996年到 2004年,Dr。 Allen 一直在英国剑桥Babraham 研究所神经生物学部门从事研究工作,包括DNA/RNA操纵、提纯/分离、电泳、PCR、克隆、印迹工作、文库筛选、遗传图绘制、转基因设计与构建、人工染色体工程、用于ES细胞基因打靶的载体设计与构建、细胞培养、切片技术、小鼠行为分析、小动物外科手术以及序列数据库和分析工具等生物信息工具的研究。

The world's venture capitalists, having fed on the computing boom of the 1980s, the internet boom of the 1990s and the biotech and nanotech boomlets of the early 2000s, are now looking around for the next one.

全世界敢冒风险的资本家,上世纪80年代依托计算机的飞速发展、90年代靠互联网的繁荣、21世纪初凭生物技术和纳米技术的小景气发家,现正在寻找下一个目标。

A virus is a unit of infectious genetic material smaller than any bacteria and embodying properties placing it on the borderline between life and non-life. The vast majority of the virus capsid protein assembled into icosahedral symmetry of the structure. Caspar-Klug Theory has become a fundamental concept for the classification of icosahedral viral capsids based on the principle of quasi-equivalence.

二:病毒是比任何细菌都小的感染性遗传物质,介于生命与非生命之间的一类无细胞结构的生物,绝大多数病毒的衣壳蛋白装配成二十面体对称的结构,&准等价&原理是解决严格遵从二十面体对称性的病毒衣壳几何特征的有效而成熟的方法。

Keywords Empoascini,Fauna,Taxonomy,Biogeography,Phylogeny,Cladistic analysis,new genera,new species,new combinations,new records,new synonyms,China.

小绿叶蝉族;区系;分类;生物地理;系统发育;支序分析;新属;新种;新组合;新记录;新异名;中国

Bio-Module units should be preceded by primary clarifiers, combined primary clarifier and sludge storage (lmhoff-tank), or fine-screening devices.

生物模块设备的前面是初沉池,组合式初沉池和污泥池,或者是小格栅设施。

Therefore liquid CO_2 avoids gas-solid multiphase flow, and supplies continuous low temperature gas for cryoprobe. Experimental results show that the 2-dimension temperature distribution for the first time, it includes:(1) The cooling velocity and freezing zone increases along the flux up at certain size of the cryoprobe in the same biological tissue.(2) As the diameter of cryoprobe increases, the temperature will descend faster, freezing zone will be broader obviously, and ice ball become larger. Until it arrives heat balance, the temperature keeps invariability.(3) Single freezing-thaw results in biggish mechanical injury already for loosen tissue such as pork liver; Successive freeze-thaw circle could quicken freezing and thaw velocity for compact tissue, and it has proved the more mechanical injury of successive freeze-thaw circle than the single freezing-thaw treatment.

文中首次对生物组织进行了二维温度场分布实验研究,得出:(1)当刀头大小一定,对同一组织冷冻时,流量越大,降温速度越快,降温影响区域越大;(2)随着冷刀直径的增加,降温速度随着增加;温度影响区域明显增大;冰球直径也随着增大,热量平衡后温度几乎不随时间变化;(3)对于结构疏松组织比如猪肺脏,单次冻融对组织已造成较大的机械破损;对于结构较致密组织,多次反复冻融可以使降温和复温速率增加,单次冻融的热应力造成的机械破损较小,多次冻融后造成的机械破损比单次冻融所造成的机械破损大;(4)直喷式刀头比导热式节能约15%。

Using an in itro bioassay, we demonstrated that cytokine-actiated microglia release cytotoxins that kill retinal neurons.

应用体外生物测定技术,我们证明细胞因子活化的小神经胶质细胞释放杀死视网膜神经元的细胞毒素。

Dragons are dangerous creatures, and they like to live in the same kinds of caves that Dwarves do.

龙都是很危险的生物,它们喜欢栖息在和小矮人一样的洞穴里。

Egrets, gulls and small mammals are the predominant wildlife on the island these days.

白鹭、海鸥和小哺乳动物在这些年成了岛上的主要野生生物。

As one tiny non-coding RNA, miRNA distributes in the eukaryote with a length of 21~25 nucleotides.

微RNA是非编码小RNA中的一种,长度约21~25个核苷酸,它广泛存在于真核生物中。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

I'm running my simile to an extreme.

我比喻得过头了。