小生物

Worms of the class Oligochaeta, including the earthworms and a few small freshwater forms.

寡毛纲动物任一寡毛纲环节软体虫，包括蚯蚓和一些小的淡水生物种类

Integrated analysis based on stratigraphy, sedimentary facies and paleophysiography data indicate that small basin-mountain pairs formed in the Kuche Basin during early Mesozonic, and large basin-mountain pairs.

地层、沉积相，生物古地理等综合分析表明库车盆地中生代早期形成了小波长盆山共轭对，中晚期为大波长盆山对。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Tetrahydroisowuinolin derivatives and phthalide derivatives were designed and synthesized on the basis of a PDE4 inhibitor pharmacophore model set up through computer simulation. Some tetrahydroisowuinolin derivatives synthesized were evaluated on their activity against PDE4 for study on the structure-activity relationship.(7-cyclopentyloxy)-6-methoxy-3,4-dihydroisoquinolin-2(1H)-yl(4-hydroxy-3-methoxyphenyl)methanone (155) and Methyl 7--6-methoxy-2-(4-methoxy- benzoyl)-1-phenyl-1,2,3,4-tetrahydroisoquinoline-3-carboxylate (170) showed good activity against PDE4, which were chosen as the lead compounds to design and synthesize another analogs in order to develop selective PDE4 inhibitors with better activity.

分别设计合成了以四氢异喹啉和氨基苯酞为骨架的两类小分子杂环化合物，并对部分四氢异喹啉衍生物测试了其相关的生物活性，初步确定了其构效关系，发现了两个对PDE4有较好抑制活性的先导化合物(7-cyclopentyloxy)-6-methoxy-3，4-dihydroisoquinolin-2(1H)-yl(4-hydroxy-3-methoxyphenyl)methanone (155)和Methyl 7--6-methoxy-2-(4-methoxy- benzoyl)-1-phenyl-1，2，3，4-tetrahydroisoquinoline-3-carboxylate (170)。

Effects of control Plutella xylostella,Pieris rapae were 75.9%～83.5% and 46.9%～57.1% of Myzus persicae was controlled at same time.Rectify effect of control Aphis me...

在低浓度时对天敌生物影响更小，是高毒高残留农药理想的替代品种，可以大面积推广应用。

These results also provedthe presence of the same acute phase response profile in amphioxus,as observed ineutherians.Thus it pushed the evolutionary existence of the eutherian-like acute phaseresponse back to the protochordate lineage.Fourthly,we determined CAs,including epinephrine、norepinephrin anddopamine,with HPLC method optimized after LPS challenged amphioxus.Theresults demonstrated that E,DE and DA are present in amphioxus humoral fluid,andE was firstly found in amphioxus.

第三，我们用生物化学方法，选取海洋中广泛存在、众多水产动物的常见致病菌-溶藻弧菌作为天然反应原感染文昌鱼，从另外一个角度探讨并丰富文昌鱼体液中的八种炎性蛋白的急性时相反应，结果显示，与LPS感染文昌鱼相比，六种正APPs的浓度变化幅度相对较小，曲线相对平缓，但仍然呈正急性时相反应，蛋白电泳也证实了这一点；而二种负APPs：RBP和白蛋白(Albumin，ALB)浓度实验周期内总体呈下降的趋势，呈负急性时相反应，结论与上述LPS相同。

The bioassay results showed that parthenolide had nematicidal activity against tested nematode Bursaphelenchus xylophilus and Panagrellus redivivus, and their death rate at 200 μg/ml within 48 h were 70.4% and 100% respectively.

生物活性测定结果表明小白菊内酯对所测试的松材线虫和全齿复活线虫具有毒杀作用，浓度200μg/ml对两种线虫进行处理，48h的死亡率分别为70.4%和100%。

METHODS: Thirtysix eyes of 36 patients with refractory glaucoma underwent trabeculectomy with AMT and releasable sutures. The visual acuity, intraocular pressure, anterior chamber and filtering blebs were observed.

将临床收治的36例(36眼)难治性青光眼患者施行小梁切除术联合巩膜瓣下生物羊膜植入术并应用可松解缝线技术，术后观察其视力、前房、滤过泡和眼压等情况。

The heat pipe technology is one of the most efficiency method to solve the key technique for hard problems in order to gain high efficiency of resource utilization and good economy benefit in the application of renewably energy,In this paper,characteristics of the heat pipe technique is introduced,and its application and exploration in the solar energy,subterranean heat energy,living material energy are reviewed.

笔者结合热管技术特点，综述了其在太阳能、地热能、生物能三大能源利用领域中的国内外进展。1热管技术的技术特点[1](1)高的导热性。热管内部主要靠工作液体的汽液相变传热，热阻很小，因此具有极高的传热效率。(2)优良的等温性。

Dr. Sheau-Farn Yeh's lab found that taiwanin A, isolated from Taiwania Cryptomeriodes, is cytotoxic for several cancer cell lines, especially for human hepatoma Hep G2/A2 and Hep 3B/T2 cells. Besides cytotoxicity, the compound can arrest the cells at G2/M phase. Not only taiwanin A and its derivatives, but also compounds which isolated from the plane Clausena excavata and the roots of Rubia cordifolia have been found to have biological activities.

阳明大学生物化学研究所叶小帆老师实验室在筛选天然抗癌药物时，发现自台湾杉心材所分离出的一种化合物taiwanin A对於许多的肿瘤细胞株均有很强的细胞毒性，其中对人类肝癌细胞株Hep G2/A2与Hep 3B/T2，taiwanin A除了有显著的细胞毒性之外，同时也会造成细胞周期停滞在G2/M相。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。