小牛的

The pair were among a train of drivers being held up by the sluggish Sebastian Vettel, but Toro Rosso boss Franz Tost remarked that Buemi's approach to passing 23-year-old Piquet was not correct.

了两位老人之间列车的司机被扣留的呆滞维泰尔，但红牛二队老板弗兰兹托斯特说Buemi的方法通过23岁的小皮奎特是不正确的。

Calf rennet, consisting of mainly chymosin with a small but variable proportion of pepsin, is a relatively expensive enzyme and various attempts have been made to find cheaper alternatives from microbial sources These have ultimately proved to be successful and microbial rennets are used for about 70% of US cheese and 33% of cheese production world-wide.

与其他凝乳酶一起组成了一个虽小然而却有很大比率的胃蛋白酶的幼牛凝乳酶相对来说比较昂贵，因此目前关于能否在微生物资源中寻找廉价的凝乳酶正在试验中。这一设想最终一定会成功并且从微生物中得到的凝乳酶将会用于生产美国市场中77%，全球市场33%的乳酪制品。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

ConclusionThe extraction rate of volatile oils from Heracleum hemsleyanum Diels by supercritical CO2extraction process is higher than that of water vapor distillation,and the optimized extraction conditions are:extraction pressure 25MPa,extraction temperature 45℃ and flow rate of CO2 20L/h, and the yield of volatile oils is 0.65%.

结果通过各因素级差大小的比较可知：萃取压力的变化对萃取率的影响最大，萃取温度次之，而CO2流量对萃取率的影响最小。结论超临界CO2萃取牛尾独活挥发油的萃取率较水蒸气蒸馏法高，最佳萃取条件为萃取压力25MPa，萃取温度45℃，CO2流量20L/h，挥发油收率为0.65%。

The agglutination for rabbit was the highest among erythrocytes of human, rabbit, common carp and crucian carp. The hemagglutinating activity for rabbit could not be inhibited by D-fructose, D-mannose, D-galactose, glucose, sucrose, manrmn, gamma-globulin and egg albumin, but inhibited by bovine-thyroglobulin, and the minimum inhibitory concentration was 3.10 mg/ml.

该凝集素可以凝集人的A、B、AB、O型红细胞，且凝集活性相同，在对人、兔、鲤、鲫的红细胞的凝集作用中，对兔红细胞的凝集作用最强，且不被己测试的D-半乳糖、D-果糖、D-甘露糖、葡萄糖、蔗糖、甘露聚糖、γ-球蛋白、卵清蛋白所抑制，仅被牛甲状腺球蛋白抑制，最小抑制质量浪度为3.10mg/ml。

Outside, two bull-necked Afrikaners sipped tea in the garden of the adjacent coffee shop, and a Himba man in a Cal State T-shirt and a two-panel skirt - short and gathered in the front, long and straight in back - distractedly herded goats down the main street while chatting on his cellphone.

在杂货店外面，两个有着牛颈般粗脖子的非洲男人正在咖啡店旁边的花园里小口地品着茶；路边，一个辛巴男人，上身穿着一件加州风格的T恤，下身穿着两片布围成的裙子—裙子前面短而紧，后面却又长又直—他一边心烦意乱地沿着主街道赶着他的羊群，一边打着手提电话。

The main process machinery and equipment are:(1) hit halo hemp electrical appliances, due to livestock and poultry species, varieties vary;(2) assassination put Xuedao, because different types of livestock and poultry, knives of different sizes, in order to ensure that medical and consumption of blood cleaning hollow knife can be used;(3) peeling or burning hair back hair machinery and equipment, medium and large slaughter plant machinery stripped of pigs, cattle, sheep skin, small factories and individual butcher knife while peeling by hand, Mao 60-68 ℃ hot hot hot flooding 3-8 minutes, easy to back hair, animal hair may be back with a different machine back hair;(4), cut open, cuts except internal organs, cutting off their heads and trimming hooves many different processes such as cutting tools complete with manual and electric saws and a half split two ways , hand-split and a half with machetes, saws chop and a half should be washed clean bone debris.

主要工序机械设备是：（1）击晕麻电器，因畜禽种类、品种不同而异；（2）刺杀放血刀，因畜禽种类不同，刀的大小也不同，为保证医用和食用血液的清洁，可采用空心刀；（3）剥皮或烫毛退毛机械设备，大中型屠宰厂用机器剥去猪、牛、羊的皮，小厂和个体屠户则用手工刀具剥皮，烫毛用60—68℃热水浸烫3—8分钟，便于退毛，畜禽均可用不同的退毛机器退毛；（4）开膛、剖除内脏、割头蹄和修整等工序多用不同的刀具完成，劈半有手工和电锯两种方法，手工劈半用砍刀，电锯劈半后应将骨屑冲洗干净。

A flat roof covered with sheets of lead .Turning towards the prow, one had before one an innumerable flock of ancient roofs, over which arched broadly the lead-covered apse of the Sainte-Chapelle, like an elephant's haunches loaded with its tower.

你一转向船头，呈现在面前的是一片无边无际的古老屋顶，仿佛是一群铺天盖地的牛羊，而浮现在其上面的是圣小教堂后殿的铅皮圆屋顶，远望过去，好似一只大象后背上驮着教堂的钟楼。

Turning towards the prow, one had before one an innumerable flock of ancient roofs, over which arched broadly the lead-covered apse of the Sainte-Chapelle, like an elephant's haunches loaded with its tower.

你一转向船头，呈现在面前的是一片无边无际的古老屋顶，仿佛是一群铺天盖地的牛羊，而浮现在其上面的是圣小教堂后殿的铅皮圆屋顶，远望过去，好似一只大象后背上驮着教堂的钟楼。

The half dome over an apse .Turning towards the prow, one had before one an innumerable flock of ancient roofs, over which arched broadly the lead-covered apse of the Sainte-Chapelle, like an elephant's haunches loaded with its tower.

你一转向船头，呈现在面前的是一片无边无际的古老屋顶，仿佛是一群铺天盖地的牛羊，而浮现在其上面的是圣小教堂后殿的铅皮圆屋顶，远望过去，好似一只大象后背上驮着教堂的钟楼。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。