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小梁形成

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Result:As compared with the model group, the effect of small dose group and middle dose group of osteopractic total flavone on TBV% of shankbone increased significantly; the effect of small dose group and middle dose group of osteopractic total flavone on TRS% of shankbone reduced significantly and TFS%, AFS%, MAR, BFR of shankbone reduced obviously; the effect of middle dose group of osteopractic total flavone on OSW and mAR reduced obviously, the effect of small dose group of osteopractic total flavone on them had the tendency of reducing, but there was no statistical significance.

结果 :对胫骨骨小梁体积百分比的影响,与模型组比较,骨碎补总黄酮小剂量组和中剂量组的TBV %明显增高;对胫骨骨小梁吸收表面百分比的影响,与模型组比较,骨碎补总黄酮小剂量组、中剂量组的TRS %显著降低;对胫骨骨小梁形成表面百分比,活性生成表面百分比,骨小梁矿化率和骨小梁骨生成率的影响,骨碎补总黄酮小剂量组、中剂量组的TFS %,AFS %,MAR ,BFR较模型组皆明显降低;对胫骨类骨质平均宽度和骨皮质矿化率的影响,骨碎补总黄酮中剂量组的OSW和mAR与模型组比较,明显降低;骨碎补总黄酮小剂量组与之相比,有下降的趋势,但无统计学意义。

Four weeks after operation, a large quantity of rosy newly formed bone and green bone trabecula were showed in the BG-COL-PS-HYA group. At week 12, scaffold materials was substituted by new bone tissues basically, and Haversian system was visible. Eight weeks after operation, the results of X-ray film revealed that cortical bone was fully connected.

生物活性玻璃/胶原蛋白/透明质酸/磷酸丝氨酸组术后4周硬组织切片可见大量玫瑰红色新骨和绿色骨小梁形成,术后12周支架已基本由新生骨组织替代,哈弗系统形成;术后8周X射线显示骨皮质连接完整,12周缺损完全修复,髓腔基本再通。

Microscope examination:in group A,at 2 weeks,a few inflamatory cells appeared in the holes with many osteoblasts and ostoid appeared around the drilled holes.At 8 weeks,marrow formed in the drilled holes in group B,at 2 weeks,there were a large amount of osteoblasts in the drilled holes and some ostoid formed around thc drilled holes.At 4 weeks,the drilled holes were full of trabeculae.At 8 weeks,the trabeculae matured with the marrow appeared in the intertrabecular space.

组织学结果:2周时A组钻孔区出现少许炎症细胞,边缘出现较多成骨细胞并有骨组织形成,至8周时,钻孔区内形成骨髓组织,只在边缘形成骨小梁结构。2周时B组钻孔区有大量的成骨细胞,边缘有较多骨组织形成,4周时钻孔区内充满重生骨小梁结构,8周时钻孔区内骨小梁成熟,小梁有骨髓组织填充。

After operation, spinal fusion was achieved in 10 cases within 5 months to 7 months, 6 months on average, and pseudoarthrosis in 1 case was found by the CT examination.

术后12 个月X 线片检查示融合节段有连续骨小梁形成并通过椎间隙10 例,1 例术后CT 扫描证实前方有假关节形成。

New bone trabecula formation could be observed in experiment group, after 8~12 weeks. There were little blood vessel on periphery.

组织切片HE染色观察见对照组无新骨形成,有纤维包裹,实验组8、12周时有新骨小梁形成,周围有较多小血管形成。

Histological observation showed that bone trabecula had formed in the experimental group, but no new bone formation in the control group at 12 weeks after operation.

组织学观察显示实验组术后12周可见新生骨小梁形成,对照组术后12周未见新生骨小梁形成

Obstruction from nodular prostatic hyperplasia has led to prominent trabeculation seen on the mucosal surface of this bladder with hypertrophy.

结节性前列腺增生引起的阻塞致显著的小梁形成,可在过度增生的膀胱粘膜表面见到。

This led to obstruction with bladder hypertrophy, as evidenced by the prominent trabeculation of the bladder wall seen here from the mucosal surface.

从膀胱壁粘膜表面明显的小梁形成可以看出阻塞使膀胱肥大。

RESULTS: During 10 - 12 weeks, in cuntrol group: The defect area was repaired by white and soft tissue that had no resistance to press. The repaired tissue was still lower than the surrounding articular surface with clear boundary. By histological observation, it was found that the defect was repaired by the mechanism similar to inflammatory reaction and the defect is ultimately filled by the hyperplasia of hyaline degenerative fibrous tissues. In filling group: the defect was repaired by semi-transparent, smooth, textured tissues with polish that had resistance to press as well as elasticity. The repaired tissue was almost similar to the shape of the surrounding cartilage,difficult to be distinguished. After histological observation, it was found that there was no inflammatory reaction, but active hyperplasia of inner bonetissue and cartilage tissues; a lot of osteoid tissues and trabeculation were found. Newlborn cartilage was fused with surrounding cartilage tissue and connected with surrounding tissues.

结果:10~12周,对照组:缺损区由白色、质软、按压无阻抗的组织修复,修复组织仍低于周围关节面,边界仍清晰可辨,组织学以类似炎症反应的机制修复缺损,最终以透明变性的纤维组织的增生来填补缺损部位;填充组:缺损区由半透明状、质韧光滑有光泽,按压有阻抗并有弹性的组织修复,修复组织与周围软骨外形上已基本相似,不易区分,组织学未见有炎症反应的过程,内骨组织和软骨组织增生活跃,并可见大量类骨组织和骨小梁形成,新生软骨和周围软骨组织融合,并与周围组织连接。

The data of femur biodymamics showed that acupuncture and moxibustion could increase the biggest load, modulus of elasticity, the biggest flexibility. The data of shank bone morphometry showed that acupuncture and moxibustion could increase percentage of bone trabecula bulk, lessen percentage of bone trabecula absorption and restoration, slow the rate of bone trabecula mineralization. Slices indicated that the structure of bone trabecula became basically complete.

股骨骨生物力学参数显示,针灸可提高OP症大鼠股骨最大载荷、弹性模量和最大挠度;胫骨骨组织形态计量学指标显示,针灸可增加骨小梁体积百分比,减少骨小梁吸收表面百分比和骨小梁形成表面百分比,降低骨小梁矿化沉积速率;胫骨骨组织切片显示,针灸后大鼠骨小梁结构已基本完整。

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