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Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果:经槲皮素处理的人结肠癌SW480细胞数量减少,部分细胞体积缩小,细胞膜完整,胞浆浓缩,核染色质固缩,细胞核碎裂,形成凋亡小体;MTT法检测显示当作用浓度为30μmol/L~90μmol/L时,槲皮素对人结肠癌SW480细胞的生长有抑制作用,其抑制作用随着作用浓度的增加和作用时间的延长而增强;流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期,大部分细胞被阻断于S期,随药物浓度的升高和作用时间的延长,G0/G1期细胞比例逐渐增加,S期细胞比例逐渐减少;酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9,随浓度的升高,MMP-2及MMP-9的分泌量减少;免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后,Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Machining process and general information:· General Information – Tooling o Plan to have own engineering group as company expands o Hwasung is subcontracted to do engineering currently o Uses same cutting tools as in Korea facility – all diamond o Regrind tools if can o Hwasung has capability to surface grind, repair dies, and make molds with wire EDM process · General Information – Machining o Boundary samples are used: okay, borderline, not good o Quality room has separate windows for incoming and outgoing parts o New equipment will be coming in from FM LaGrange plant o Each operation has pictures and job instructions for what to check and which gage to use to check part o Parts are manually loaded into machine or pallet, depending on line o Auditor checks parts for verification: after operator checks part, puts in bin, auditor checks part and reloads back on line o Have tool management system o A pokeyoke check is used for orientation prior to loading o Machines are guarded o Mostly have CNC machines o Manual chip collection systems o If automated, have robots with grippers to load/unload parts from pallets o After final inspection, operator visually inspects and loads onto plastic dunnage with the crown up and loads into the washer o Repairs: filing down of burrs or sharp edges after anodizing the crown o Have 2 Excello machines for special machining of the pin bores o 2.7% scrap for gasoline pistons o Cycle time is 18 seconds for gasoline piston o Process – Wash o Spray system o 4 stations: heated wash, wash with degreaser, 2 rinse stations o Uses RO water o Operator blow dries piston and puts on dunnage with crown up o Parts wheeled into coating room o Needs major improvements here o Chemical analysis done for bath in chemical room o Comments/Observations: o Relatively small piston facility, but plans for additional space.

加工工艺和一般信息:·–的一般信息工具 o 有自己的工程集团,随着公司的扩展计划 o Hwasung 是分包做当前工程 o 用途相同的扦插工具,如在韩国设施–所有钻石 o 回收料工具如果可以 o Hwasung 已表面研磨,修复模具,和模具丝电火花加工能力过程·一般信息–加工 o 界限示例用于:好交界,不好 o 质量房间有单独的窗口传入和传出的部件 o 新设备会在来从调频拉格朗日植物 o Each 操作具有图片和工作说明的什么复选,计为使用检查部分 o 部件是手动加载到机器或托盘,取决于行 o 审计检查验证零件:置于 bin,运算符检查部分后,核数师检查部分并重新加载回行上 o 有工具管理系统 o 为 pokeyoke 复选用于在加载之前的定位 o 机被保护 o Mostly 有数控机床 o 手动芯片收集系统 o 自动,如果有与要加载/卸载部分从托盘 grippers 机器人 o 后最终检验,运算符将直观地检查并加载到与塑料衬垫冠了与到洗衣机负荷 o 修复:提交下毛刺或锋利的阳极氧化冠后 o 有 2 Excello 机特别加工的销孔 o 2.7%废料的汽油活塞 o 周期时间是为汽油活塞式 18 秒 o 过程–洗 o 喷射系统 o 4 站:加热洗、与脱脂剂洗、 2 冲洗站 o 使用反渗透水 o 运算符吹干活塞式并将了放冠衬垫 o 部件轮式进涂层房间 o 需要重大改善在这里 o 为在化学的房间里浴做的化学分析 o 注释/结论: o 相对小活塞式设施,但额外的空间的计划。

This study examined whether viral vectors could be used to deliver the BDNF gene in a less damaging fashion and whether this could promote a regenerative response in injured rubrospinal neurons.

本研究检测了病毒载体是否可以用来在比较小的损伤模型上促进BDNF基因的释放,以及它是否可以激发受损红核脊髓神经元的再生反应。

This study examined whether iral ectors could be used to delier the BDNF gene in a less damaging fashion and whether this could promote a regeneratie response in injured rubrospinal neurons.

本研究检测了病毒载体是否可以用来在比较小的损伤模型上促进BDNF基因的释放,以及它是否可以激发受损红核脊髓神经元的再生反应。

objective to investigate the effect of radiography and method of operation on the surgical indication as well as the operation treatment of lumbar disc herniation and lumbar canal stenosis.methods the data of 300 cases with lumbar disc herniation and lumbar canal stenosis were reviewed from jan 1999 to dec 2004.all cases were treated by the method of minimal invasive open operation.the nucleus was resected through an 3.5~4.5cm in length.the sacrospinalis muscle between laminae was stripped partly and the part of upper lamina and the part of medial facet was resected.

目的 探讨影像学检查和手术方法对腰椎间盘突出症和椎管狭窄症手术方式和适应证的影响。方法自1999年1月~2004年12月,为300例腰椎间盘突出症和椎管狭窄症患者实施微创开放式手术,做3.5~4.5cm长的皮肤切口,剥离椎间的部分骶棘肌,咬除部分椎板上缘及小关节内侧缘后,摘除髓核,并根据需要做半椎板或全椎板手术或扩大神经根管和切除肥厚的黄韧带及神经管内的骨性增生。

The histopathology showed that the tumors were rich in cells arranged in fascicles with bipolar and spindled processes. The nuclei showed minor pleomorphism. A few tumor cells were centered on the vessels with a nuclear-free zone that was characteristic of ependymoma.

病理组织学上肿瘤富于细胞,排列成束状、交织状,细胞胞突细长双极,核异型性小,少数肿瘤细胞围绕血管周围形成室管膜瘤的无核区结构。

Flowers subsessile; bracteoles and sepals more than 40, not clearly differentiated.

花近无柄;小苞片和萼片超过40,不清楚使有差异。 4 P。 oblongicarpa 长核果茶

At the age of 19 weeks, we have observed ciliated-mucous cells, some cells presented with mucous granules in the supranuclear cytoplasm and a ciliating border with short growing shafts, while the other cells presented with large mucous granules as well as compound cilia.

小粘液颗粒细胞呈柱状,胞浆顶部含有少量球形的粘液颗粒,细胞表面有短小的微绒毛,细胞核大呈椭圆形,胞浆电子密度高,胞浆下部有粗面内质网和线粒体,核上部有高尔基体。

Methods Twenty consecutive patients with hypertensive intracerebral puta-men hemorrhage were treated by making a incision on sclap long about 6 cm across sylvian fissure,making a small bone flap about 3 cm ×3 cm,After opening dual,we injected water under microscopic control by a handheld syringe ...

对术后4~12个月内的日常生活能力进行分级。结果无1例死亡,生存者的ADL分级为:1~2级10例,3级5例,4级4例,5级1例。结论Toth水解剖技术用于小骨窗经侧裂入路手术治疗高血压壳核出血简单、安全、有效。

Objective To study the pathogenesis and clinical symptoms of patient with central type of interˉvertebral disc protrusion manifested intermittent claudication as the mainly clinical presentation,so as to reach the early diagnosis and treamtmet in time.Methods In58cases,X-ray film,lumbar discography,CT examinations were done to confirm the diagnosis,then total laminectomig was performed to reveal the vertebal body without involving small arˉticular process.Intervertebral tissue was excised via duralotomy approach in2cases in remaining56cases,through right and left longitudinal syndesmotomy to resect the nucleus pulposus of lumber intervertebral disc.Results In58cases treated by operation,the operative findings were in according with the X-ray and CT examinations ofpre-opˉeration.

目的 对呈间歇性跛行主要临床表现的中央型椎间盘突出症患者的发病机制和临床表现进行研究,达到早诊断、早治疗方法对58例患者进行X线拍片,腰椎碘造影,CT扫描检查,根据检查结果确诊后,采用全椎板切除显露椎体,未累及小关节突,2例经硬膜切开入路切除间盘组织,余56例经从双侧用神经剥离子带棉片将硬脊膜带神经根向中线剥拉至显露圆形突出物止,分别从左右纵韧带摘除髓核。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

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