小核

The basical ideas, arithmatic methods and applied conditions of reduction of partial least squares and discrete wavelet transformation, logitboost, random forests and fuzzy kendal discriminate analysis of microarray gene expression data, ordinal discriminate analysis of time series multivariate data and varied-coefficient-logistic-regression-model discriminate analysis of the data with varied regular pattern in itself.

本文对医学领域中判别分析方法的新进展做一综述，介绍了微阵列基因表达数据判别分析中偏最小二乘法降维、离散小波变换法降维、logitboost算法、随机森林、模糊核判别分析以及时间序列多元数据有序判别分析法、自身有变化规律数据的变系数logistic回归模型判别分析法的基本思想、算法和适用条件。

Partial least squares regression method is adopted to realize robust regression of MLS-SVM.

利用偏最小二乘回归方法对多核最小二乘支持向量机进行了鲁棒回归。

They have distinctive compositions, and display the compositional trends: from Mg and Ca-enrichment towards Fe-enrichment from big phenocryst—the core of small phenocryst—the rim of small phenocryst and microcrystals, typical characterisitc of tholeiite series.

这三种类型的辉石在成分上有明显的差异，从大斑晶辉石—小斑晶辉石核部—小斑晶辉石边部和基质辉石，成分由富镁、钙向富铁方向演化，显示出拉斑玄武系列的特点。

The three groups were loaded and analyzed under the same loading according to the mean value of the bite force of incisors. RESULTS:(1)The maximum stress of dentin in group A was twice higher than that in group B. The maximum primary stress, minimum primary stress and maximum shear stress of group A were respectively 236.35, 228.83 and 218.05 percent of those in group B. The difference of maximum stress values of group B and group C was neglectable.(2) The maximum stress distribution of dentin in group A and group B was quite different (the stress was concentrated in labial and lingual side of the cervical dentin in group A, otherwise in group B it was concentrated in the area around the alveolar and the labial and lingual side of the dentin which was opposite to the tip of the cast metal post and core). The maximum stress distribution of dentin in group A and group B was almost the same.

结果：(1)从牙本质的应力大小来看，A组中牙本质最大应力值比B组中牙本质最大应力值增大了1倍以上（最大主应力、最小主应力和最大剪切应力A组分别是B组的236.35%、228.83%和218.05%），而B组与C组的牙本质最大应力值相差极小；(2)从牙本质的应力分布位置来看，A组与B组的牙本质最大应力分布位置相差甚远（A组主要集中在牙颈部唇舌侧肩台部的牙本质上，B组主要集中于牙槽嵴顶附近及铸造桩核尾端相对应的唇舌侧的牙本质上），而B组与C组的牙本质最大应力分布几乎在同一位置。

In this thesis, the main problem investigated is the characterization of the image space in the wavelets transform by making use of the relationship between wavelets transform and the theory of reproducing kernel.

利用小波变换像空间与再生核空间的联系，本文主要研究了小波变换像空间的描述问题。

Histological study indicated that low temperature inhibited microspore formation and caused abnormal multiplication of tapetum layer cells.

经切片观察，低温会造成绒毡层细胞的异常增殖，及产生多核性巨大小孢子，而阻止小孢子发育。

From tetrad stage to microspore stage, the ABA content in sterile buds was higher than fertile buds.

四分体到小孢子单核期和小孢子二核后期至成熟花粉期不育花蕾ABA含量仍高于可育花蕾。

Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells.

Rab蛋白是一类小分子的GTP酶，调节真核细胞内的小泡运输。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Results In Hyacintn bletilla group fibroblastic cell hare small soma,a little spine.

结果 白及胶组成纤维细胞胞体较小，胞突少，多呈圆形，胞核较小。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。