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AMs that collected, pured and cultured with contine method were stimulated by LPS of different concentration(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml) for 60min or by 1μg/ml LPS for different time stage (0min,5min,15min,30min,60min,120min) to observe the dynamic change of NF-кB intranuclear level and NO production, from which the best concentration and time point of LPS stimulation were selected. In the study, all AMs were divided into 4 groups: control group, group stimulated with LPS, group interrupted by Cal C and group inhibited by PDTC. The following parameters were measured: NF-кB level in nuclear protein extraction of AMs detected with sandwich ELISA, Inter-nuclear transposition of NF-кB observed with immunocytochemistry staining, NO content in cell culture medium quantitied with nitric acid reductase assay, Morphologic change of AMs in apoptosis observed with acridine orange staining and fragmentation at genome DNA of AMs detected with apoptotic electrophoresis assay.

分离、纯化及培养大鼠肺巨噬细胞;以不同浓度的LPS(0μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml)和不同作用时间(0min,5min,15min,30min,60min,120min)分别刺激小室培养的细胞单层,观察NF-κB的核内浓度及NO合成量的动态变化,选择LPS的最佳用量和作用时间;然后分成四组实验,设正常对照组,LPS处理组,特异性PKC抑制剂阻断组,NF-κB抑制剂阻断组;收集培养的单层细胞及培养液;采用夹心ELISA法定量测定细胞核提取物中的NF-κB水平;免疫组化法检测NF-κB的核内移位变化;硝酸还原酶法测定细胞培养液中NO含量;吖啶橙染色观察凋亡细胞的形态学变化,凋亡电泳实验检测细胞凋亡后基因组DNA的断裂情况。

The organization structure of experimental group was disorganized, cracked and the bone trabecula was rarefactive and non-intact and there was a great number of empty lacuna in bone trabecula. While there was the reverse situation in the control group .

结果实验组组织结构紊乱,破碎,骨小梁稀疏、不完整,有量的空骨陷窝,而对照组骨小梁相对较完整、排列整齐,骨小梁中的骨细胞清晰可见,核大,位于中央。

ResultThe organization structure of experimental group was disorganized, cracked and the bone trabecula was rarefactive and non-intact and there was a great number of empty lacuna in bone trabecula. While there was the reverse situation in the control group .

结果实验组组织结构紊乱,破碎,骨小梁稀疏、不完整,有大量的空骨陷窝,而对照组骨小梁相对较完整、排列整齐,骨小梁中的骨细胞清晰可见,核大,位于中央。

Resultthe organization structure of experimental group was disorganized, cracked and the bone trabecula was rarefactive and non-intact and there was a great number of empty lacuna in bone trabecula.

结果实验组组织结构紊乱,破碎,骨小梁稀疏、不完整,有大量的空骨陷窝,而对照组骨小梁相对较完整、排列整齐,骨小梁中的骨细胞清晰可见,核大,位于中央。

A lot of organelles such as mitochondria, rough endoplasmic reticula and Golgi bodies were observed in fiber cell. Then, double karyotheca disappeared, and the organelles disintegrated. Multivesicular bodies appeared in fiber cytoplasm. With the further development of fiber, fiber wall underwent continual thickening with aging, and polylaminate structure gradually appeared. While the agglutinated nucleus, transfer vesicles, plasma membrane and plasmodesmata still presented.

研究发现,次生壁形成早期,细胞核具有双层核膜,染色质凝聚,可见大量的线粒体、粗面内质网和高尔基体等细胞器存在于纤维细胞中;随后,双层核膜消失,细胞器将逐渐解体,多泡体开始出现在纤维细胞的细胞质;随着年龄的增加,纤维细胞壁逐渐增厚,并出现多层结构现象,而运输小泡、细胞膜、胞间连丝和凝聚的染色质将持续存在。

The groundwork for emo was laid by Hüsker Dü's 1984 landmark Zen Arcade, which made it possible for hardcore bands to tackle more personal subject matter and write more tuneful and technically demanding songs. Emo emerged in Washington, D.C. not long after, amidst the remnants of the hardcore scene that had produced Minor Threat and Bad Brains.

除了Dischord的中坚地位,多数早期的情感核都很地下,那些流星般出现的乐队在小厂牌下录制发行少量的黑胶唱片;有些演唱者在舞台上演唱到歌曲的高潮部分会情到深处而潸然泪下,而引起纯硬核音乐分子的嘲笑。

Microinjection of norepinephrine (NE, 30 nmol) into the subnucleus commissuralis of nucleus tractus solitarii of rabbits evoked a decrease of mean arterial pressure and heart rate. These effects were attenuated by application of naloxone (3 nmol) on the same area,which by itself showed no effect on MAP and HR.

于杂种白兔的孤束核联合亚核内微量注射去甲肾上腺素,引起血压降低和心率减慢,将小剂量阿片受体阻断剂纳洛酮注入CNTS,本身不影响血压与心率,但减弱NE在CNTS中的降压降率幅度。

Pit corrosion is a main kind of localized corrosion. The process of forming pit corrosion includes three stages-formation of pit source, development of pit cave and forming occluded corrosion cell. Molybdate series inhibitors can be inhibitive, for it can control three stages of pit development. First, there is apparent synergistic effect between molybdate and phosphate, which can suppress formation of pit source. Second, there is apparent passivation of molybdate inhibitor according to electrochemistry analysis, which let oxidation film in unoccluded cave repassivate.

首先,钼酸盐与磷酸盐或硅酸盐复配的缓蚀剂具有较理想的缓蚀性能,并且有明显的协同作用,从而能抑制部分点蚀核的产生;其次,钼酸盐复配缓蚀剂的使用,从电化学的角度分析,具有明显的钝化性能,且钝化区间较宽、维钝电流密度较小,从而能使未封闭的蚀孔或点蚀核附近的氧化膜再钝化,得到有效的修复;最后,对于还有部分蚀孔发展成为闭塞腐蚀电池的情况,钼酸盐、磷酸盐能进入蚀孔内部并且在酸性条件下生成铁钼磷酸盐,从而有效地减缓了点蚀的恶化,抑制了腐蚀的发展。

After the cells dealt with GS-Rh2 of 13.0 mg/L, the typical apoptosis cells were discovered: showing disorganized, highly contracted and vacuolated, condensed cytoplasm, vanished karyotheca, ruptured cell nucleus and apoptotic body.

凋亡细胞吉姆萨染色检测:经13.0 mg/L人参皂甙单体Rh2处理后,HL60细胞出现核染色质浓缩、呈周边凝聚,核膜裂解形成凋亡小体、胞质出现空泡但胞膜完整的典型细胞凋亡形态学改变。

In eukaryotes, DNA left-handed chirality by the way, wrapped around histone octamer around, the formation of a stable nucleosome structure.

在真核生物中,DNA按左手手征性的方式,缠绕在组蛋白八聚体的周围,形成稳定的核小体结构。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。