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In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离,建立了高效的特异性毒素分离纯化方法,并对其进行了紫外波谱分析;用荧光素双醋酸酯染色法,测定了毒素特异性组分对原生质体死亡率的影响;进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究,旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白,为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

If the setting is too low, scanned images granula edge rough, radiofrequent denticular, quality is poor; if it is set too high, you can make to the original copy in the unnecessary details, such as by blotchs, fold, and additional background around the image exposed, may also make the scanned images of storage space is too large, affecting the scan speed.

如果撤销太矮,扫描的图像颗粒粗劣,图像边际呈锯齿状,质量很差;如果撤销太矮,则会使草稿洋不必定的梗概,如画背上的斑不背、褶皱以及图像边际的其他配景突显不入来,还会使扫描图像的保存不时间过不小,感化扫描速率。

Eagle's type has very much, for example: Big spot vulture, condor, black yuan and so on The eagle and the common birds are a little different, the eagle belongs to the animal class, The common birds belong to the omnivora animal, not too same, This is the experienced bird height 56 centimeter, the chord approximately 417-465 centimeter, the body weight approximately 630-1030 gram, egg 2-5, Bai De has the reddish brown spot approximately, sits on eggs contract 38 day,…A thorough material eagle secondary next four, five small hawks, because their lair is very high, hawk and common birds' difference The eagle for black yuan in Taiwan's popular name, the Taiwanese calls Ye Huoli the wing.

老鹰的种类有很多,例如:大斑鹫、秃鹰、黑鸢等欢迎来到本站,老鹰和一般鸟类有点不同,老鹰属於禽兽类,一般鸟类属於杂食类的动物,不太相同,这就是老成鸟身长约56公分、翼长约417-465公分、体重约630-1030公克、蛋2-5个,白底有红棕色斑点、孵卵期约38天、。。。深入资料老鹰一次生下四、五只小鹰,由於它们的巢穴很高,鹰和一般鸟类的不同老鹰为黑鸢在台湾的俗称,台语称来叶或厉翼。

The HRCT findings in patients with BOOP include: 1 patchy consolidation (seen in 80% of cases) or ground-glass opacity (in 60% of cases), often with a subpleural and/or peribronchial distribution; and 2 small ill-defined nodules (30-50% of cases) which may be peribronchial or peribronchiolar.

HRCT上BOOP的表现包括:1、斑片状实变(见于80%的病例)或毛玻璃样影(见于60%的病例),通常位于胸膜下和/或支气管旁;和2、边界不清的小结节(见于30~50%的病例),可于支气管旁或细支气管旁。

The major dominant species of predatory enemies were Erigonidium graminicola and Theridion octomaculatum and their individuals were 81.4 percent of the total predatory enemy individuals of the community.1.2 Spatial patterns and their dynamics of major dominant predatory enemies ofarthropod communityThe research results showed that Pieris rapae distributed mainly on heart leaves and back of inner leaves of cabbage plants, Plutella xylostella distributed mainly on the back of cabbage leaves and Lipaphis erysimi distributed widely in cabbage plants. Among the major dominant species of predatory enemies, Sitticus sinensis distributed mainly on the front of leaves of cabbage plants in spring cabbage fields, and Misumenopos tricuspidata and Propylaea japonica distributed widely in cabbage plants.

在捕食性天敌中蜘蛛10种,捕食性昆虫3种,主要优势种为草间小黑蛛、八斑球腹蛛,其数量占捕食性天敌总数量的81.4%。1.2节肢动物群落主要优势种的空间格局及其动态研究结果表明,害虫优势种菜青虫主要分布于甘蓝心叶和甘蓝叶反面,小菜蛾主要集中分布在甘蓝叶的反面,而菜蚜在甘蓝植株内的分布较广;春甘蓝田捕食性天敌优势种中华跃蛛在外层叶正面分布较集中,三突花蛛与瓢虫类在甘蓝植株内的分布较广。

Screening should generate an even after Rose leaf-shaped structure, and the world could not make a moire patterns these mesh point structure will pass a small image details and see the wide range of halftoning, produce high quality images.

增网后答该生不败平均的玫瑰斑形的网不面布局,而不克不及生不败莫尔纹,这些网不面布局不兵传达洪不小的图像梗概和广宽的阶调边界,制息不入矮质度的图像。

Phylogenic analysis based on entire S1 gene sequences showed that majority isolates clustered together with the close phylogenetic relationships. Therefore, we researched pathogenicity of the selected isolate IBV CK/CH/LDL/05II in this group, Pathogenic study showed that this virus caused 100% morbidity and 54.5% mortality to 15-day-old specific-pathogen-free.The kidneys from the dead chickens showed the predominant gross lesions which were pale, mottled, and swollen. The renal tubules and ureters were distended with excess urates.

经S1基因系统进化树分析发现,本实验所分离的大多数IBV毒株(8/12)分布于同一基因进化群内,并且亲缘关系较近,因此,我们对此群中IBV毒株CK/CH/LDL/05II的致病性进行研究,以10~(5.5)×EID_(50)/0.1ml的病毒接种15日龄的SPF雏鸡,鸡群发病率为22/22(100%),死亡率为12/22(54.5%),死亡鸡以肾脏肿大、苍白、外观呈现典型的&花斑肾&,肾小管和输尿管扩张并且有尿酸盐沉积为主要病理变化。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。