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We investigated the c-fos protein expression in nucleolus after the shear stress applied and found c-fos protein expression.

还利用流动小室方法对在剪应力作用下细胞核内原癌基因c-fos蛋白的翻译水平的表达进行了初步研究,结果提示大鼠成骨细胞在剪应力作用下,c-fos蛋白有明显的表达。

Pollen sac A chamber in which the pollen is formed in the angiosperms and conifers.

花粉囊:在被子植物和针叶类中形成花粉的小室

When they had finished their song, the woman in white moved towards the prompter's box, and a man, with his stout legs encased in silk tights, with a plume and a dagger, went up to her and began singing and waving his arms.

当她们唱完这支歌以后,那个身穿白连衣裙的少女走到提词人小室前面,那个粗壮的腿上裹着一条紧身绸裤的男士,手里拿着一顶饰有一根白羽的帽子和一柄匕首,走到她跟前,两手一摊,唱起歌来。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry,FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时,对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后,Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR,RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时,Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope,LSM)观察北豆根总碱(0、40μg/ml)作用24小时后,Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Her beautiful face wore an expression like that of the stony countenancewith the snaky ocks.

仆人们领着他走过富丽的大厅和小室一直到她的房间里去——这里最华贵的东西就是她。

The model takes account of CFB boiler components such as the dense and dilute region, gassolid separator, standpipe and recycle device in detail. Several main parameters and features of CFB boiler are calculated.

同时以小室概念为基础,结合相应的数值计算方法对锅炉不同运行工况下的循环回路一些主要参数的动态过程进行了大量的模拟计算。

MethodsA subline of human pancreatic cancer cell line MIAPaCa2 (MIAPaCa23) with high liver metastasis potential was selected and injected through the tail vein to establish a pancreatic cancer model in nude mice with high liver metastasis. The capability of invasion in vitro was evaluated by a transwell chamber system.

方法筛选出高肝转移的胰腺癌细胞亚型MIAPaCa23,用Transwell小室模型观察PESV对MIAPaCa23细胞体外侵袭能力的影响;尾静脉注射MIAPaCa23细胞建立高肝转移率的裸鼠胰腺癌模型,对比PESV和化疗药物吉西他滨以及抗血管生成剂TNP470对裸鼠胰腺癌肝转移的抑制作用。

Methods The high invasiveness subseries cell lines were selected based on the penetrating power of artifical basilar membrane and polycarbonate membrane through constructing invasive cabin.

通过构建侵袭小室,以穿透人工基底膜、聚碳酸脂膜能力为依据筛选具有体外高侵袭能力的膀胱癌细胞亚系。

Tetsuya Komuro million of the swindled money, there used to repay part of the cost.

小室哲哉骗来的五亿巨款中,就有一部分拿来偿还了这笔费用。

Japanese pop music days later, Tetsuya Komuro was the producer of her.

日本流行音乐天后,小室哲哉是她的制片人。

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I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。