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Cut the fish fillet into the small chunks, marinate with salt for 25 minutes.

将鱼肉改切成小块,放入碗内,加盐腌上25分钟

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖,琼脂0.7%,pH5.8 培养基中25℃暗培养20d 后继代一次,诱导产生Ⅱ型胚性愈伤组织,用镊子将其夹碎成2mm2大小的小块,置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中,侵染时间为45min,共培养3 天后,转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天,再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中,遮光培养20d后,置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化,卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%;同时以甘蔗心叶愈伤组织材料,通过循环水式真空泵,产生负压,设定不同的负压值,在农杆菌菌液中感染5min 后,在负压条件下继续侵染15min 后,筛选出抗性愈伤组织并获得转化植株,其中在负压为-0.05 时转化率达到最大值,其卡那霉素抗性愈伤组织获得率平均为37.5%。

But there are offbeat neighborhoods to explore and old-fashioned towns nearby where you can still savour something of bygone days.

但附近还有小块的地区可以供游客进行探索,老式风格的城镇也仍然能让你欣赏到逝去岁月中的一些东西。

Finally, cut the omelette into pieces and serve it with some cheese on top.

最后,把鸡蛋切成小块,在上面撒上芝士端出去。

Such as are viviparous are hair-coated, and such as are oviparous are covered with a kind of tessellated hard substance; and the tessellated bits of this substance are, as it were, similar in regard to position to a scale.

象胎生这种的,是有体毛的,象卵生这种的,体表覆盖一种带花纹的坚硬物质;并且这种带花纹的物质小块,仿佛某种程度上分布的位置类似。

Parboil the fresh chicken in boiling water and drain.

先将鸡肉洗净切小块,用滚水汆水,沥乾。

In this case, which looks at the relationship between sales and scrap volumes, over time, the data is inferred through a simple arithmetic triangulation between sales volume and parc size.

在这种情况下,看关系在销售之间和小块容量,在时间期间,数据被推断通过简单的算术三角测量在销售量和parc 大小之间。

Peasants in one commune there secretly started parcelling out land, expecting death for it, but soon gained backing from a provincial leader and Deng ally, Wan Li. Others gradually followed suit.

一个公社的农民将土地秘密分成小块耕种,他们预计到有可能因此丧生,但是他们的行动却很快得到了该省领导、邓的盟友万里的支持。

Proponents of reform argue the parcelling out of small plots of land to households and the rigid laws that forbid private sale or purchase of contracted land mean agriculture remains small-scale and inefficient and ties down the country's more than 730m rural residents at a time when the government is encouraging rapid urbanisation.

支持改革的人士指出,将小块土地分给农户,并通过僵硬的法律禁止个人买卖承包经营的土地,意味着中国农业依然停留在小规模和低效率的模式,而且在政府鼓励快速城市化之际,使逾7.3亿农村居民难以流动。

Using parchment paper overhang, lift out of pan, and transfer to a cutting board.

可以提着羊皮纸将蛋糕从烤盘中取出,放到一块砧板上,用有锯齿的刀将之切成16小块

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?