小周期

Young Modulus of Kitan clay was measured during initial loading, after creep deformation, within small cyclic loading and global unloading-reloading.

Kitan粘土的杨氏模量分别在最初加载、蠕变后，小的周期加载和滞回圈时测定。

Big pharmaceutical firms such as Merk or Eli Lilly gain from such partnerships because the smaller firms typically development new drugs in as little as five years,versus an eight-year average development cycle in the larger firms.

如礼来公司默克或从这种伙伴关系得到大制药公司，因为小企业发展的新的药物通常在短短的5年，相对于8年的开发周期平均在较大的公司。

ObjectiveTo evaluate the efftct of hormone therapy and sequently inducing ovulation treatment in nulliparous women with the premabure ovarian failure,and to explore the possible effective ovulation inducing scheme.

小 目的通过对未生育妇女卵巢早衰患者进行周期治疗和促排卵治疗，评价其疗效，探讨可能有效的促排卵方案。

Under appropriate conditions the existence and the continuous dependence of its homoclinic orbits and periodic orbits on the perturbing small function are obtained.

在一定条件下，得到了同宿轨与周期轨的存在性，即这一类系统在这类小参数函数扰动下是结构不变的。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

These results also provedthe presence of the same acute phase response profile in amphioxus,as observed ineutherians.Thus it pushed the evolutionary existence of the eutherian-like acute phaseresponse back to the protochordate lineage.Fourthly,we determined CAs,including epinephrine、norepinephrin anddopamine,with HPLC method optimized after LPS challenged amphioxus.Theresults demonstrated that E,DE and DA are present in amphioxus humoral fluid,andE was firstly found in amphioxus.

第三，我们用生物化学方法，选取海洋中广泛存在、众多水产动物的常见致病菌-溶藻弧菌作为天然反应原感染文昌鱼，从另外一个角度探讨并丰富文昌鱼体液中的八种炎性蛋白的急性时相反应，结果显示，与LPS感染文昌鱼相比，六种正APPs的浓度变化幅度相对较小，曲线相对平缓，但仍然呈正急性时相反应，蛋白电泳也证实了这一点；而二种负APPs：RBP和白蛋白(Albumin，ALB)浓度实验周期内总体呈下降的趋势，呈负急性时相反应，结论与上述LPS相同。

The main girder is designed to apply C60 pumpable high performance concrete with set retarding, high early strength and good volume stability to avoid cracking and meet the time requirement for the project.

主梁混凝土设计为C60泵送混凝土，采用8米挂篮悬浇工艺施工，混凝土技术要求很高：不仅坍落度损失要小、易于泵送，而且还要具有较长的缓凝时间，以保证一个梁段一次浇筑完成；有较高的早期强度，可在3至4天内进行预应力张拉，以缩短节段悬浇周期；有较好的体积稳定性，以避免梁体产生有害变形和开裂。

Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果：经槲皮素处理的人结肠癌SW480细胞数量减少，部分细胞体积缩小，细胞膜完整，胞浆浓缩，核染色质固缩，细胞核碎裂，形成凋亡小体；MTT法检测显示当作用浓度为30μmol/L~90μmol/L时，槲皮素对人结肠癌SW480细胞的生长有抑制作用，其抑制作用随着作用浓度的增加和作用时间的延长而增强；流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期，大部分细胞被阻断于S期，随药物浓度的升高和作用时间的延长，G0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少；酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9，随浓度的升高，MMP-2及MMP-9的分泌量减少；免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后，Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Machining process and general information:· General Information – Tooling o Plan to have own engineering group as company expands o Hwasung is subcontracted to do engineering currently o Uses same cutting tools as in Korea facility – all diamond o Regrind tools if can o Hwasung has capability to surface grind, repair dies, and make molds with wire EDM process · General Information – Machining o Boundary samples are used: okay, borderline, not good o Quality room has separate windows for incoming and outgoing parts o New equipment will be coming in from FM LaGrange plant o Each operation has pictures and job instructions for what to check and which gage to use to check part o Parts are manually loaded into machine or pallet, depending on line o Auditor checks parts for verification: after operator checks part, puts in bin, auditor checks part and reloads back on line o Have tool management system o A pokeyoke check is used for orientation prior to loading o Machines are guarded o Mostly have CNC machines o Manual chip collection systems o If automated, have robots with grippers to load/unload parts from pallets o After final inspection, operator visually inspects and loads onto plastic dunnage with the crown up and loads into the washer o Repairs: filing down of burrs or sharp edges after anodizing the crown o Have 2 Excello machines for special machining of the pin bores o 2.7% scrap for gasoline pistons o Cycle time is 18 seconds for gasoline piston o Process – Wash o Spray system o 4 stations: heated wash, wash with degreaser, 2 rinse stations o Uses RO water o Operator blow dries piston and puts on dunnage with crown up o Parts wheeled into coating room o Needs major improvements here o Chemical analysis done for bath in chemical room o Comments/Observations: o Relatively small piston facility, but plans for additional space.

加工工艺和一般信息：·–的一般信息工具 o 有自己的工程集团，随着公司的扩展计划 o Hwasung 是分包做当前工程 o 用途相同的扦插工具，如在韩国设施–所有钻石 o 回收料工具如果可以 o Hwasung 已表面研磨，修复模具，和模具丝电火花加工能力过程·一般信息–加工 o 界限示例用于：好交界，不好 o 质量房间有单独的窗口传入和传出的部件 o 新设备会在来从调频拉格朗日植物 o Each 操作具有图片和工作说明的什么复选，计为使用检查部分 o 部件是手动加载到机器或托盘，取决于行 o 审计检查验证零件：置于 bin，运算符检查部分后，核数师检查部分并重新加载回行上 o 有工具管理系统 o 为 pokeyoke 复选用于在加载之前的定位 o 机被保护 o Mostly 有数控机床 o 手动芯片收集系统 o 自动，如果有与要加载/卸载部分从托盘 grippers 机器人 o 后最终检验，运算符将直观地检查并加载到与塑料衬垫冠了与到洗衣机负荷 o 修复：提交下毛刺或锋利的阳极氧化冠后 o 有 2 Excello 机特别加工的销孔 o 2.7%废料的汽油活塞 o 周期时间是为汽油活塞式 18 秒 o 过程–洗 o 喷射系统 o 4 站：加热洗、与脱脂剂洗、 2 冲洗站 o 使用反渗透水 o 运算符吹干活塞式并将了放冠衬垫 o 部件轮式进涂层房间 o 需要重大改善在这里 o 为在化学的房间里浴做的化学分析 o 注释/结论： o 相对小活塞式设施，但额外的空间的计划。

And during the course of construction mental structure products" designing and manufacturing, there"re some widespread problems such as long design cycle, high error rate, difficult to total the material, large amounts of information, and time after time rehandling, etc.

但是我国建筑金属结构产品制造企业较普遍地存在着企业规模小，且其产品设计周期长、出错率高、材料统计费时费力、重复劳动工作量较大。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。