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First, the German cockroach has a larger number of eggs per capsule than the other species that infest structures.

首先,德国小蠊在生殖结构上,它的每个卵荚含有的卵比其它物种要多得多。

Achenes ovoid, obovoid, or slightly to strongly bilaterally compressed, smooth, sometimes tuberculate or spiny, sometimes marginate or winged along sutures, usually greenish, black in R. melanogynus. Seeds with a copious endosperm and small embryo.

瘦果卵圆形,倒卵球形的,稍到强烈两侧压缩,平滑,有时具瘤的或者多刺的,种子具一大量的胚乳和小的胚。

Eggs of Black-crowned Night Heron and Little Egret were sampled in May of 2005 from Yingshang Balihe Reserve, Anhni Province. Residues of Cd, Pb and Cr were determined by atomic absorption spectrophotometer analysis.

2005年5月,采集了淮河地区颍上八里河集群繁殖的夜鹭和小白鹭鸟卵共18枚,用原子吸收法测定了卵壳、内容物中重金属Cd、Pb、Cr的残留量。

Branches strigose; leaf blade ovate or narrowly ovate, margin dentate, denticulate, or serrulate, with entire tip; spikes usually less than 26 cm.

分枝具糙伏毛;叶片卵形或狭卵形,边缘具牙齿,具小齿,或有细锯齿的,具全缘的端部;穗状花序通常不到26 厘米 35

Leaves tufted; stipes 3-10 cm long, glabrous, narrowly winged near apex; lamina oblong-ovate to triangular, generally 10-12 cm long, bi- or tripinnate; pinnae 3-5 pairs, oblong-ovate, sessile; rachis narrowly winged; pinnules pinnately dissected, segments linear.

叶簇生;叶柄长3-10毫米,无毛,近顶端有狭翅;叶片长卵圆形至三角形,通常长10-12厘米,二回或三回羽状分裂;羽片3-5对,互生或近对生,长卵圆形,无柄,羽轴有狭翅;小羽片羽状细裂;末回裂片细长,线形。

Erect small tree or shrub; petals 6-10; inflorescences 2-7-flowered; leaf blade ovate to broadly ovate or suborbicular

直立小乔木或灌木;花瓣6-10;花序2-7花;叶片卵形到宽卵形或近圆形 10

Nutlets usually light brown, straight, ovoid or narrowly ovoid, tuberculate or smooth; attachment scar at base.

通常的小坚果浅褐色,直,卵圆形或者狭卵球形,具瘤或平滑;附件痕在基部。

Fruit fleshy or dry, globose or ovoid, umbilicate, with areoles, glochids, and sometimes spines.

侧膜胎座果肉质或干燥时,卵球形卵球形,肚脐状的,具小窠,倒剌毛,和有时具刺。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达,且在未成熟卵母细胞中,MⅠ期的表达明显强于GV期的表达;②RT-PCR产物克隆测序结果显示:昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp~1153bp)]完全一致;Blastn比对结果发现:在不同物种中差异较小,选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析,提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%,100%,100%,36%,100%,100%,67%,100%,100%,92%,99%,99%,99%;Clustal 1.81和Treeview构建出的分子进化树表明:在上述14种生物中昆明小鼠与中国仓鼠进化关系最近,与兔子、猪聚成一簇,与食蟹猴进化关系最远;③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段,重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Nutlets 4, all or some developed, turbinate to ovoid or triangular-ovoid and dorsiventrally compressed; abaxially usually discoid, ribs or margin usually winged, dentate or with glochids.

小坚果4,全部或者一些发展,陀螺状的到卵球形卵圆形或三角形并且被背面压缩;背面通常盘状,肋骨或者边缘通常飞行,有齿或者用钩毛。

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