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First 27 rabbits of the experimental group were destabilizated by operated on L6、7.Inj Diazepam 0.25 mg/kg,Inj Ketamine 0.02 g/kg and Inj Atropine 0.125 mg/kg was one by one injected into the rabbit through the auris vein,shearing the rabbit hair of backside waist,fixing the rabbit on operation table in face lying,using 1% Povidone Ioding to degerm the operation area.Every rabbit was incised at the backside of its waist,that incisal opening is located in the center of the intervertebral space (L6、7 space) that both side iliac crest line correspond ,from the meso-ordinate direction cut about 4 cm incision,cutting open skin and subcutaneous tissue,thoroughly ,exposing the spinous processes the vertebral plates and the upper-inferior articular processes,entirely segregating the muscles that cohere the spinous processes the vertebral plates and the articluar process,then excising the supraspinal ligaments and interspinal ligaments,biting off two sides the inferior articular processes of L6,in order that resulting in intervertebral destabilization,using 0.9% Inj.Sodium Chloride to washout the incisal opening,in order sewing up each layer tissues.

方法] 取48只6个月龄日本大耳白兔,雌雄不限,体重为(2.5±0.2) kg,随机进行分组,分为对照组和实验组,对照组为21只;实验组为27只;先将实验组兔腰背部皮毛剪除,用安定注射液1.25 mg/kg、氯胺酮0.02 g/kg、阿托品0.125 mg/kg顺次耳缘静脉注射麻醉后,俯卧固定于手术台上,用1%碘伏消毒手术区域,以髂嵴平对椎间隙(即L6、7)为中心,从正中取一长约4 cm纵行切口,切开皮肤及皮下组织,锐性分离,暴露棘突、椎板及上下关节突,将附着于棘突、椎板及小关节的肌肉全部分离开,然后依次切除L6、7棘上及棘间韧带,咬除第6腰椎两侧下关节突,造成椎间失稳,用无菌生理盐水冲洗切口,依次缝合各层组织;术后动物在笼中自由活动。

Inj Diazepam 0.25 mg/kg,Inj Ketamine 0.02 g/kg and Inj Atropine 0.125 mg/kg was one by one injected into the rabbit through the auris vein,shearing the rabbit hair of backside waist,fixing the rabbit on operation table in face lying,using 1% Povidone Ioding to degerm the operation area.Every rabbit was incised at the backside of its waist,that incisal opening is located in the center of the intervertebral space (L6、7 space) that both side iliac crest line correspond ,from the meso-ordinate direction cut about 4 cm incision,cutting open skin and subcutaneous tissue,thoroughly ,exposing the spinous processes the vertebral plates and the upper-inferior articular processes,entirely segregating the muscles that cohere the spinous processes the vertebral plates and the articluar process,then excising the supraspinal ligaments and interspinal ligaments,biting off two sides the inferior articular processes of L6,in order that resulting in intervertebral destabilization,using 0.9% Inj.Sodium Chloride to washout the incisal opening,in order sewing up each layer tissues.

方法] 取48只6个月龄日本大耳白兔,雌雄不限,体重为(2.5±0.2) kg,随机进行分组,分为对照组和实验组,对照组为21只;实验组为27只;先将实验组兔腰背部皮毛剪除,用安定注射液1.25 mg/kg、氯胺酮0.02 g/kg、阿托品0.125 mg/kg顺次耳缘静脉注射####后,俯卧固定于手术台上,用1%碘伏消毒手术区域,以髂嵴平对椎间隙(即L6、7)为中心,从正中取一长约4 cm纵行切口,切开皮肤及皮下组织,锐性分离,暴露棘突、椎板及上下关节突,将附着于棘突、椎板及小关节的肌肉全部分离开,然后依次切除L6、7棘上及棘间韧带,咬除第6腰椎两侧下关节突,造成椎间失稳,用无菌生理盐水冲洗切口,依次缝合各层组织;术后动物在笼中自由活动。

Results A dense accumulation of fos and jun-positive neurons exhibited in the PVN, SON and DG, and some of fos and jun-positive neurons were also present in other hypothalamus and hippocampus areas including parvocellular PVN, dorsomedial nucleus and lateral hypothalamic area.

结果 游泳应激结束后3h,室旁核和视上核及齿状区内可见密集深染的c-fos和c-jun阳性细胞,室旁核小细胞部,背内侧核等区域亦有疏密不一的阳性胞体。

The elastic strains induced in the epicentral region by the passage of the seismic wavefield generated by the largest of the nuclear tests, the May 11 Indian test with an estimated yield of 40 kilotons, is about 100 times smaller than the strains induced by the Earth's semi-diurnal (12 hour) tides that are produced by the gravitational fields of the Moon and the Sun.

由核试验产生的震动波经过而诱发了在震中区域的弹性应变。5月11日估计有40千吨级的印度的核试验,比由月球和太阳引力引起的12小时一次的潮汐引发的应变小100倍。

Many proteins are associated with epididymosomes and the protein composition of these vesicles varies along the excurrent duct and differs from soluble intraluminal proteins.

许多蛋白与epididymosomes相关联,小泡的蛋白质组成随着附睾管腔区域的不同而改变,而且不同于附睾管腔内的可溶蛋白成分。

The key research progresses and results that we accessed by the project were as follows: 1 A pair of degenerated primers were designed in the conserved domain which based on the amino acid sequence of HAK in Arabidopsis thaliana. The 500 bp fragment of HAK were amplified from the roots of Puccinellia tenuiflora by RT-PCR. The full sequence of HAK were obtained by 5' and 3' RACE methods and were determined and analyzed.

本项目主要取得了以下研究进展和成果:(1)根据已有HAK转运蛋白氨基酸序列在保守区域设计简并PCR引物,用反转录PCR方法,扩增出500bp的小花碱茅根HAK片段,通过5''和3''RACE的方法获取了该HAK的全长序列,完成了测序和序列同源性分析。

In the first frame, ROI of high-frequency subbands was detected by morphological Harr wavelet lifting and connectivity analysis.

在第一帧内,利用形态Harr小波提升和连通性分析检测出高频子带中的感兴趣区域。

Consequently, this paper presents a texton-based algorithm of texture image segmentation. At first, Harr wavelet is utilized as a transform tool so that texture subimages of different directions are produced.

首先 ,以 Harr小波为变换工具,得到具有方向性的纹理子图象;然后给出了一种新的纹理基元提取方法,并在此基础上,应用统计方法和矢量场,对纹理区域进行由粗到细的分割。

Twelve weeks following repair, coronal defect regions were filled with callus. 3D reconstruction showed that defects were repaired. Defect region and surrounding bony tissues had bony connection, with thick bone trabecula and mature Haversian system.

修复后12周,冠状位CT显示缺损区基本被骨痂填满,三维重建示缺损基本修复;缺损区域和周围骨组织形成骨性结合,骨小梁粗大,哈弗氏系统成熟。

In this paper,a novel clustering algorithm DBSB (Density Based Spatial Clustering Method with Heuristically Selecting Border Object) is proposed.The algorithm fastly expands the clusters by a heuristical function to choose core objects in the border region of the known core object,and then merge some clusters by border objects.

本文提出了一种启发式选择边界对象的快速空间聚类算法DBSB,通过一个启发式函数近似选择相对于某个已知核心对象边界区域中的核心对象和边界对象,通过核心对象的序列来快速地扩展它们所在的簇,直至找到一些较小的簇。

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