小体外的

The fluence and frequency of laser was controlled precisely, which could melt and destroy the adiposis tissue without damage to the adjacent tissues. The histological study of treated adiposis tissues, post-operative reaction and clinical results were observed and studied. Results Local adipose tissues were melted and liquefied effectively by the laser. Compact fibrous tissues of cellulite were broken by the laser into loosen structure. The bleeding was lower than traditional method due to the coagulation of capillary vessels. The advantages of laser lipolysis included less edema, petechia and complications, which produced a rapid recovery and satisfactory results.

但该方法也存在疼痛、血管损伤、出血较多[1]等不足，此法主要是利用机械力插入负压吸引管，通过较强的负压将脂肪组织吸出体外，比较适用于大范围、厚层次和疏松的脂肪吸除，难以应用于小范围局限致密的脂肪组织，如面颈部和手臂部等，这些部位脂肪构成较特殊，包含有大量坚韧的呈网状交织的纤维结缔组织，质地较紧密，普通负压难以吸除，吸脂管插入和抽动阻力较大，如强行反复抽吸对组织创伤明显，因此，有必要寻找一种更加有效的治疗方法。

By using the technique of tissue culture, we successfully obtained the cell from goldfish fin, skin, eye, heart, spleen, spermary, swim bladder and kidney. Among them, GFFhas the strongest proliferating ability.

采用组织块法和胰蛋白酶消化法对金鱼细胞进行体外培养和传代，研究了高倍稀释、培养液中的小牛血清浓度以及冷冻保存等因子对显示出较高的增殖能力的来源于鳍的细胞GFF的影响进行了试验；并对其进行金鱼疱疹病毒接种、病毒传代培养等试验和电子显微镜观察。

Percutaneous transhepagtic drainage of gallbladder was a safe and simple technique, it drained infected bile equally as surgical cholecystostomy; and it was of benefit to aged or patient of high surgical risk.

经皮穿肝胆囊引流术是一种不需要手术，即可提供相当於胆囊造瘻术的技术，在局部麻醉下很快地把感染的胆汗引流体外，令临床症状得以快速改善；由於此技术的安全简便及侵袭性小，对於需要紧急进行胆囊造瘻术的年老病人或高手术危机者，有著极大的裨益。

The electrolytic production are the middle matters of organic chloride and final matters of organic chloride besides dicarbonate . This may limit the application of electrochemical technology .Must consider the production and control of organic chloride when electrolysis organic wastewater concluding chloride ion.

苯酚和腐植酸在氯离子体系中的电解产物除了有CO_2这样的小分子气体外，还生成了有机氯中间体和最终产物，这可能会限制电化学氧化法的应用，在电解处理含氯的有机废水时必须考虑有机氯的形成和控制问题。

Shen tonics and nourishing Yin were selected, and decided into the groups of liquid of complex prescription by the Yin-Yang principles of the treatment: IQ and NY group, YT and NY group, ST and NY group and so on, which were respectively boiled, administrated the rats via gavage and separated the sera of drugs for preparation, the sera were served in HCSMMC-7721 in vitro, normal sera of rats and Retin acid as matched controls, MTT colorimetric method was applied to assay cell proliferation.

选取临床肝癌治疗常用的益气、温阳、补肾、养阴等扶正治法的代表药物，并根据阴阳治则理论将其分别配伍为益气养阴、温阳养阴及补肾养阴等治法的小复方，分别煎成汤液，采取大鼠灌胃给药后分离药物血清的方法制备药物血清，作用于体外培养的SMMC-7721人肝癌细胞，以正常大鼠血清和维甲酸作对照。

Methods MSCs were cultured with inbred line small-ear cancellous bone of Ban Na pigs . A 1.5 centimeter segmental defect was created in the mid-upper part of the radial shaft of adult rabbit. The defects were implanted into composited xenogeneic bone in experimental group and xenogeneic bone alone in control group , the defects were not filled with anything in blank group . The repair capability of the defects was assessed by scan electron microscope before operation and physical , histology, X-ray , transmission electron microscope, SPECT and bone mineral density examinations 4 , 8 and 12 weeks after operation .

将MSCs与版纳近交系小耳猪松质骨在体外联合培养，兔桡骨中上段制成1.5cm的骨—骨膜缺损模型，实验组植入复合异种骨，对照组植入单纯异种骨，空白对照组不植入任何材料，分别于术前行标本的扫描电镜观察和术后4周，8周，12周各时间点行标本的大体观察，组织学观察，X线片观察，透射电镜观察，SPECT扫描及骨密度测试，比较其骨缺损区骨修复愈合情况。

In vitro killing effect of adenovirus - mediated herpes simplex virus thymi- dine kinase gene under regulation of hypoxic response element on hepatoma cell line HepG2WANG Xiao - zhong, PENG Qi - quan, PENG Yun - heng, LIAO Wen - peng Department of Hepatobiliary Surgery, Central Hospital of Shantou City, Shantou 515031, China.

王小忠 彭启全彭云恒廖文鹏广东省汕头市中心医院肝胆外科，广东汕头515031摘要：目的：构建携带受低氧反应元件调控的单纯疱疹病毒胸苷激酶基因的重组腺病毒载体，探讨该重组腺病毒对体外培养的肝癌细胞HepG2的特异性杀伤活性。

AIM: To study the effect of tumor necrosis factor-α on the expression of matrix metalloproteinases in the cultured bovine trabecular cellsand approach to pathogenesis and mechanism of primary open angle glaucoma.

摘要目的：观察肿瘤坏死因子-α对体外培养的牛眼小梁细胞MMP3，MMP9和TIMP-2表达的影响，探讨原发性开角型青光眼的发病机制。

Inseminated in 8-9 hrs after GVBD, the ova have the highest hatching rate (more than 86% in the concerned spawn).Based on the above insemination research of the oocyte maturation in vitro, recombinant opAFP-pUC19 was microinjected into the germinal vesicle of oocyte of maturation in vitro.

经过卵母细胞时期在生发泡中注射外源抗冻蛋白基因的体外成熟卵，其受精率（70～80%）和孵化率（38～75%）均低于对照组10～30%，在进行外源抗冻蛋白基因注射后授精孵出的小鱼长至半成体和成体鱼后，分别用斑点杂交和Southern blo-tting分析外源基因的整合。

Neural stem cells, defined by Reynols 1992, which were derived from the adult mice corpus striatum firstly, can uninterruptly proliferate and multidifferentiate in vitro and in vivo.

神经干细胞(neural stem cells，NSCs)是干细胞的一种，Reynolds等于1992年首先从成年小鼠脑纹状体分离出能在体外不断分裂增殖、具有多种分化潜能的细胞群，并正式提出了神经干细胞的概念。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。