小体外的

This experiment was conducted to investigate the effect of glutamine on growth performance of 21 days weaning piglets, and approach the mechanism from internal secretion levels, small intestine morphology, tissue DNA content, T lymphocyte proliferation and differentiation rate, serum immunoglobulin levels and so on.

中文摘要：本试验研究日粮中添加谷氨酰胺，对21日龄断奶仔猪在断奶后不同阶段生长性能的影响，并从，小肠道切片观察，组织DNA含量，体外T淋巴细胞增殖分化，血清抗体水平、内分泌水平等方面来探讨谷氨酰胺对断奶仔猪的作用机理。

Methods Primary metanephric mesenchyme stem cells were cultivated and multi-directional differentiation was induced.

方法原代分离培养后肾间充质干细胞，在体外特定诱导条件下观察细胞向成骨细胞、脂肪细胞及肾小管上皮细胞的分化及其形态演变，采用细胞免疫荧光检测细胞表面特定蛋白的表达变化。

Objective: To study the growth inhibition and radiosensitivity effect of Gemcitabine on non-small cell lung cancer cells.

目的：研究吉西他滨对体外培养非小细胞肺癌细胞的细胞周期改变和凋亡影响，及其放疗增敏的作用及机制。

Objective To study the preparation of berberine tannate colon-specific coated tablets, and investigate the effects of in vitro release conditions on the colon-targeting effects of the coated tablets and colon-targeted release.

目的 研究鞣酸小檗碱结肠定位包衣片的制备工艺，并考察体外释放条件对包衣片结肠定位及结肠释放行为的影响。

Objective To study the stimulating effect of activin A on the transdifferentiation of renal tubule epithelial cells cultured in vitro.

目的 研究激活素A对体外培养的人肾小管上皮细胞转分化的影响。

As noted above,membrane-stabilizers unexpectedly improved the heart function andprevented ER stress both in vivo and in cultured cells.We know that the membranestability depends on the sodium homeostasis in fact.To investigate whether ER stress isassociated with sodium channel action,which determins the membrane stability,wepretreated H9c2(2-1) cells with veratridine,a sodium channel activator.

上面的研究发现膜稳定剂在体内体外均抑制了内质网应激，并改善了心脏功能，而膜稳定作用实际上是维持细胞钠离子稳态的结果，为了研究内质网应激反应与钠离子活动的关系，我们用一种钠通道激活剂藜芦定刺激H9c2(2-1)细胞，30分钟时GRP78表达增加；24小时时PERK磷酸化和CHOP蛋白表达显著增加，说明钠离子通道过度激活诱发了内质网应激反应。

To investigate the inhibitory effect of a small peptide from Agkistrodon acutus venom on the growth of human gastric carcinoma SGC-7901 cell line and its mechanism.

目的 探讨尖吻蝮蛇毒小肽对体外培养的人胃癌SGC-7901细胞株的增殖抑制作用及作用机制。

Moreover, our group could show increased oxidation of extracellular glutathione in vitro by cultivated alveolar macrophages from patients with fibrosing alveolitis, both spontaneoulyand after PMA stimulation.

而且，我们对从纤维性小肺泡炎患者中获得的肺泡巨噬细胞在体外进行培养，并应用自发和PMA进行刺激，研究结果显示：细胞外谷胱甘肽的氧化增加。

METHODS: The model of tissue damaged submandibular gland in 10 rats was made by deligation. One week later, the gland tissue was obtained to harvest submandibular gland stem/progenitor cells by enzyme digestion in vitro. Following 10-14 days of primary culture, small round cells were collected, purified and subcultured for monoclonal culture.

10只大鼠采用结扎下颌下腺主导管、抑制腺体分泌来建立组织损伤模型，1周后切取腺体组织，酶消化法体外分离下颌下腺干/祖细胞，原代培养10~14 d后，挑取培养皿中形成的小的类圆形、类上皮细胞集落予以纯化，传代后进行单克隆培养。

After that, the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article, in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos.1 Effects of the reducing injury methods on IVF of cryopreservated oocytesThe result of the three methods, choosing the right time oocytes, using minimum size sample vitrification and culturing after thawing, showed that these ways could partly maintained the fertilizable competences of cryopreservated bovine oocytes.

减轻损伤方案对冷冻卵母细胞IVF的影响使用选择合适时期的卵母细胞进行冷冻；采用最小样本量玻璃化冷冻方法进行冷冻；在解冻后先孵育再进行体外受精等三种方案能够部分维持或恢复冷冻牛卵母细胞IVF受精能力。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。