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Results:①The amount of human colon carcinoma cell line SW480 treated by quercetin decreased. The morphology of partial SW480 cells was shrunk volume, integrated cell membrane, condensed cytoplasm, pyknotic chromatin, nuclear fragmentation. Apoptotic Corpuscles were found by electron microscope.②MTT colorimetric assay showed quercetin inhibited the growth of human colon carcinoma cell line SW480 in a time- and dose-dependent manner when the concentration of quercetin was 30、60、90μmol/L.③Flow cytometry analysis showed the cell cycle of SW480 cell was restricted in G1/S. G0/G1 phase rate increased and S phase rate decreased with increasing concentration of quercetin and time lasting.④ Zymogram analysis assay showed the secretion of matrix metalloproteinases in human colon carcinoma cell line SW480 treated by quercetin decreased. With increasing concentration of quercetin, the secretion of MMP-2 and MMP-9 decreased.⑤Immunohistochemistry method demonstrated the position expression of Cathepsin-D in SW480 cell was suppressed by quercetin in a time- and dose-dependent manner.

研究结果:经槲皮素处理的人结肠癌SW480细胞数量减少,部分细胞体积缩小,细胞膜完整,胞浆浓缩,核染色质固缩,细胞核碎裂,形成凋亡小体;MTT法检测显示当作用浓度为30μmol/L~90μmol/L时,槲皮素对人结肠癌SW480细胞的生长有抑制作用,其抑制作用随着作用浓度的增加和作用时间的延长而增强;流式细胞学发现槲皮素主要作用于人结肠癌SW480细胞周期的G1/S期,大部分细胞被阻断于S期,随药物浓度的升高和作用时间的延长,G0/G1期细胞比例逐渐增加,S期细胞比例逐渐减少;酶谱分析法检测显示不同浓度的槲皮素能够抑制人结肠癌SW480细胞分泌MMP-2及MMP-9,随浓度的升高,MMP-2及MMP-9的分泌量减少;免疫组织化学法显示不同浓度的槲皮素处理人结肠癌SW480细胞后,Cathepsin-D的表达随药物浓度的升高和作用时间的延长而降低。

Objective To study the relationship of prostasomes in sucecus prostaticus and motility of spermatozoa.

目的研究前列腺液中前列腺小体与精子活力的关系。

Aβ at concentration of 10 to 100 μM caused an increase in reactive oxygen species generation and the stimulatory effect was dose dependent.

当贝他类淀粉蛋白浓度由10 μM一直提升到100 μM所产生活性氧化物的量是随著浓度呈现剂量效应,但是却无法在溶小体上产生显著的脂质过氧化效应。

Furthermore, flow cytometry showed that the number of cells in G1 phase increased and the number of cells in S phase decreased, the number of cells in G2/M phases relatively increased. The changes of subcell structure could be seen, such as cavernous cells, cytoplasm agglutination, increasing apoptosis.

结果:0、10、20和40mgL^(-1),姜黄素对Hela细胞增殖的抑制率分别为3.0%、21.4%、32.8%和49.2%,且呈剂量依赖性;流式细胞术显示,G1期细胞增多,S期细胞减少,G2/M期细胞相对增多;细胞结构显示,细胞空化、染色质凝集、凋亡小体增多。

Results Curcuma (0,10,20 and 40 mg·L-1)had obvious inhibitory effects on the Hela cell proliferation in a dose-dependant manner,the inhibitory rates were 3.0%,21.4%,32.8% and 49.2%,respectively. Furthermore, flow cytometry showed that the number of cells in G1 phase increased and the number of cells in S phase decreased, the number of cells in G2/M phases relatively increased. The changes of subcell structure could be seen, such as cavernous cells, cytoplasm agglutination, increasing apoptosis.

结果:0、10、20和40 mg·L-1姜黄素对Hela细胞增殖的抑制率分别为3.0%、21.4%、32.8%和49.2%,且呈剂量依赖性;流式细胞术显示,G1期细胞增多,S期细胞减少,G 2/M期细胞相对增多;细胞结构显示,细胞空化、染色质凝集、凋亡小体增多。

Suprachiasmatic nucleus; Synapse ; Subjunctional body; 5-HT ; Degeneration ; Ultrastructure ; Immunoelectromicroscope ; Tupaia belangeri chinensis

视交叉上核;突触节下小体; 5-羟色胺;溃变;超微结构;免疫电镜;树鼠句

Results No apparent cytopathic effects could be observed during the first 24 hours of infection with L-form and elementary bodies, but the cytoplasm of some MA104 cells became vacuolated when the L-forms entering into the cells as shown by immunohistochemical staining and PCR.

结果 发现金葡菌L型及滤过的原生小体对细胞致病性降低,细胞不出现明显病变,部分细胞出现空泡样变,免疫组化及金葡菌PCR显示感染细胞内出现金葡菌抗原及DNA。

Results: Compared with that treated with anti-thyroid drugs alone, after the combined therapy, the size of thyroid decreased significantly, P<0.01. Typical apoptosis appearance, such as vacuolated cell, marginal nuclei, chromatin condensation and nuclear fragmentation, could be seen under light microscopy. The apoptotic rate before and after adding Chinese herbs was (2.11±1.78)%and (18.66±20.01)%respectively (P<0.01). TUNEL positive cells were detectable for up to 2-20 weeks after Chinese herbal treatment.

结果:与单纯抗甲状腺药物治疗比较,患者经抗甲状腺药物与中药联合应用治疗2-10周后,甲状腺组织明显缩小(P<0.01);光镜下可见细胞发泡、核着边、核固缩、核小体、核分裂等凋亡细胞的典型形态;加中药治疗前后甲状腺细胞凋亡比率分别为(2.11±1.78)%和(18.66±20.01)%(P<0.01);末端标记呈阳性。

After the cells dealt with GS-Rh2 of 13.0 mg/L, the typical apoptosis cells were discovered: showing disorganized, highly contracted and vacuolated, condensed cytoplasm, vanished karyotheca, ruptured cell nucleus and apoptotic body.

凋亡细胞吉姆萨染色检测:经13.0 mg/L人参皂甙单体Rh2处理后,HL60细胞出现核染色质浓缩、呈周边凝聚,核膜裂解形成凋亡小体、胞质出现空泡但胞膜完整的典型细胞凋亡形态学改变。

Simultaneously the disappearance of microvilli, vacuolization of cytoplasm, dispersion of nucleus and appearance of apoptotic body were observed under transmission electron microscopy. All these morphological changes are hallmarks of apoptosis.

凋亡细胞经荧光染色后表现为细胞核固缩、碎裂;透射电镜下观察发现细胞微绒毛丧失,胞内出现空泡,胞核解体及形成凋亡小体等典型的凋亡特征。

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