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The staining of brush cells of experimental group showed nuclear shrinkage, margination, nuclear fragmentation and formation of apoptotic bodies, and the characteristic ladder band on gel electrophoresis. NO changes were observed in control group.

实验组细胞刷片染色显示有明显的细胞核固缩、核周边染色质加深、核断裂、凋亡小体现象,细胞DNA电泳可见梯状条带的凋亡特征,而空白对照组无此现象。

RESULTS: There were many vacuoles and cracks in the cells, with heterochromatin margination, nuclear pycnosis and nuclear fragmentation. The high intensity pulsed electric fields caused damage in K562 cells, and the apoptotic percentage went up with the increase in frequency and intensity of PEF.

结果:经过高电压脉冲电场处理后的K562细胞,胞内出现大量空泡及裂隙,核内染色质向核周边聚集形成凋亡小体,呈典型细胞凋亡形态学改变;K562癌细胞的凋亡比例随着脉冲个数和电场强度的增加而增加。

The morphological changes in condensation and margination of nuclear chromatin, apoptotic bodies and cell necrosis significantly increased in gen group and gen plus ddp group.

形态学观察可见gen组和gen+ddp组细胞凋亡、凋亡小体及变性坏死增多,而对照组少见。

Observ ed with transmission electron microscope and scanning electron microscope, t he apoptotic nuclei showed the typical morphological feathers of apoptosis: cond ensation and margination of chromatin, bubbling of nuclear membrane and apoptoti c bodies.

在透射电子显微镜和扫描电子显微镜下都观察到凋亡的细胞核呈现典型的凋亡形态学特征,例如染色质的凝集及趋边化,细胞核表面形成空泡化结构并有大量的凋亡小体结构出现。

The apoptotic effects of LPS are detected by various skills such as fluorescence staining, TUNEL reaction, DNA agarose gel electrophoresis, electron microscopy and fluorescence activated cell sorting. Our results demonstrated that fish lymphocytes would be apoptotic under different LPS concentrations(above 10μg/ml) and different time(above 6h) inducing, which is characterized by condensation and margination of chromatin, the formation of apoptotic bodies.

结果显示,在10μg/ml以上浓度的内毒素作用18后或以100μg/ml浓度的LPS作用6h以上,淋巴细胞即可出现明显的凋亡形态学变化,表现为染色质凝集和周边化,核断裂,形成凋亡小体,DNA发生特异降解产生典型的"梯状"电泳图谱,呈现TUNEL阳性标记等,并通过流式细胞术证实这种凋亡具有时问和剂量的依赖性。

This effect may be related to the declined proliferation of melanocytes and the inhibition of melanin synthesis in melanocytes.

5%烟酰胺对UVB诱导的色素沉着有明显的抑制,它主要是通过抑制黑素细胞的增殖和黑素细胞内黑素小体的合成而发挥作用。

Results After the application of 5% nicotinamide for 4 weeks, lightening effect was obviously observed with naked eyes and the L value decreased significantly.

5%烟酰胺对UVB诱导的色素沉着有明显的抑制,它主要是通过抑制黑素细胞的增殖和黑素细胞内黑素小体的合成而发挥作用。

Methods:49 male Sprague-Dawley rats were divided into five groups: tail-suspension group I, fed with10 wt % corn oil, tail-suspension groups IK III, IV fed with three levels of fish oil (0.5, 1.5 or 3.0 wt %; low, middle and high level offish oil, respectively), normal gravity group V fed with 10 wt % corn011 served as normal control.

选用三周龄断乳雄性SD大鼠49只,采用最小体差法随机分为5组:Ⅰ组为悬吊对照组,饲喂含10wt%的玉米油饲料;Ⅱ、Ⅲ、Ⅳ组为悬吊实验组,分别饲喂含0.5wt%,1.5wt%和3.0wt%鱼油的合成饲料,并以玉米油调配各组饲料的总脂肪含量为10wt%;Ⅴ组为地面正常对照组,饲喂含10wt%的玉米油饲料。实验悬吊时间为30天。

The pineal brain sands appearing large or small mulbeny-globules, exist within either central region or peripheral position of pineal body.

松果体脑砂大小不等,常呈桑椹状球形小体;既可存在于松果体的中央区,也可位于松果体的周边部。

On the 10th ,20th ,30th,40th and 50th day, cut the tail of each mouse to make blood smears, stained the smears by Wrights method, and counted the erythrocyte containing HowellJolly bodies, or containing basophilic stipplings, or showing polychromatophilia, or showing hypochromic.

昆明种成年雄性小鼠15只,随机分成两组,实验组10只饮用2g/L硝酸铅溶液(1251mgPb2+/L)染毒,共50d。每10d切尾涂片一次,分别镜检计数含HowellJolly小体RBC,嗜碱性点彩RBC,嗜多色性RBC及低色素性RBC的出现率。

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