将发生

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

The 28S rDNA molecular phylogenetic trees showed: 1 entoprocts and phylactolaemate ectoprocts constituted a sister group, strenghthening the opinion that entoprocts should be reunited into phylum Bryozoa; 2 the cheilostome gymnolaemates was obviously non-monophyletic; 3 bryozoans itself was polyphyletic beyond any doubt, its main lineages were scattered in different positions of the lophotrochozoan trees; 4 to our great interests, the phylogenetic position of cheilostomes was between the diploblastic and the triploblastic, indicating they may play an important role during the evolution route from the diplobastic to the triploblastic animals.

28S rDNA分子系统树显示，内肛动物和被唇类外肛动物聚在一起并互为姐妹群，支持将内肛动物重新归入苔藓动物门的结论；唇口目裸唇类外肛动物绝非单系发生；苔藓动物本身也不是单系发生的，其主要类群分别位于触手冠担轮动物系统树的不同位置，特别是，唇口目苔藓动物的系统地位介于二胚层动物和其它三胚层动物之间，可能是二胚层动物向三胚层动物演化过程中的一个关键类群。

It is expected that hyg would be integrated stablely into the chromosome along with insert while spc/str would be lost during the second cross-over. Gene replacement through these two flanking sequences will result in the susceptibility of the transformants to phage φHAU3, which provides an enrichment for desired transformants by counterselection.

预期在携带有大插入片段的基因置换YAC分子进入野生型变铅青链霉菌后，YAC分子将通过1.4kb或1.0kb片段与染色体发生同源单交换，继而发生同源双交换，hyg连同大插入片段一起稳定地整合到染色体上，而spc／str丢失，从而导致菌株Hyg〓Str〓的表型。

In this case, the solidification of the liquid phase formed at the hipping temperature led to the formation of crystalline intergranular phases at all temperatures: however, the chemical compositions of the intergranular phases formed in the specimens with and without carbon additions were different, since for high carbon contents the resulting intergranular phase was observed to be richer in Yttrium(85% at Y/15% at Si).

在这种情况下，加压温度下液态产物的凝固在所有温度范围下都将生成间粒晶体：但是，是否添加了碳则将会使产物晶体的化学成分发生不同，因为在高碳含量下，产物晶体会更富含钇(85%Y比15%Si)另一方面，我们必须强调的时，如果Al2O3作为第三方物质加入反应混合物粉末中，那么产物晶体的结晶度将被破坏。

In conclusion, Kikuchi-Fujimoto disease can masquerade as a single, rapidlygrowing, parotid tumor in a middle-aged man. Because it is histiocytic necrotizing lymphadenitis adjoining the parotid gland, Kikuchi-Fujimoto disease should be considered a cause of parotid tumor.

经此案例可知，菊池－藤本氏病可以单一、快速增大，发生在中年男性且以疑似腮腺肿瘤为表徵，实际上是邻近腮腺的组织发生了组织球性坏死性淋巴结炎，故临床上耳下附近的疑似腮腺肿瘤病灶，应将本病列入鉴别诊断。

Bronchiolitis obliterans is the most disturbing complication following lung transplantation. This study is to investigate whether prolonged ischemia would promote early establishment of BO Material and Methods Left lower lobectomy was performed from miniature swine. Lung fragments, each measured 1 cm3, contained terminal bronchioles were auto- or alloimplanted into the subcutaneous area around the nipples. These lung implants were removed on 7th; 14 th; 21 th; 28th and 56th days and were fixed for histological examination. Experimental group using the same procedures but the left lower lobe was washed with 4.degree.C Lactate ringer via interlobar artery before lobar bronchus was resected.

肺移植后的阻塞性细支气管炎是肺移植后最困扰的并发症，本研究的目的希望了解缺血时间的延长是否会促进阻塞性细支气管炎提早发生实验方法施行小猪的左下肺叶切除手术，将取下的肺切成含终末支气管1 公分立方的肺小块，以自体及异体异位肺移植模式，将植入小猪皮下的肺小块於植入后第7、14、21、28 及56 天取下，在病理下检示其病理变化，并利用原位灌留的方式将左下肺叶以4℃林格氏液灌洗后再摘除之，观察有无灌洗对於阻塞性细支气管炎生成的影响。

Through designing of composition and structure of the bioactive graded coating,innerstress and its distribution in the coating were analyzed and calculated, the resultsshowed that when composition distribution coefficient n was 1.5, a reasonable stressdistribution could be got, that was at the beginning of deposition the suspension containingrichly BG granules was used so that a rich BG granules layer, a good transitional layerbetween BG layer at the bottom and the coating could be obtained at the titanium alloy side,the bottom of the coating; the stress value near the interface and surface and its character,pressure stress or tensile stress, were decided by the character of its composition itself.Changing composition distribution coefficient n could only change the variation tendency ofstress in the coating, but did not change the stress distribution rule in the coating. Thethinner the coating is, the sharper stress variation in the coating is, which does not mean thatthicker coating is better because the thicker the coating is, the little the permitteddeformation of coating is, so the coating thickness should be thinner, for example, about50μm for bending applications, but for applications only bearing pure shear stress, such asroot of tooth implant, the coating can be thicker little, for instance, about 80～100μm. The study on electrification characteristic and electrophoresis deposition of HAand BG granules in aqueous and non-aqueous solution system found that EPD almost didn'toccur in aqueous solution system. However, because HA granules take position charges inabsolute alcohol, a homogeneous EPD be carried out on the cathode titanium alloy slice, but taking negative charge in absolute alcohol the BG granules not be deposited on the cathode. A guided HA crystallizing, 100～300nm, on surface of the BG granules be realized by metathetical reaction, which cover BG granules with HA microcrystals and make the covered BG granules taking position charges in absolute alcohol, sequentially realize the EPCD of the BG and HA granules on the cathode, so it is feasible to make a titanium alloy/BG/HA bioactive graded coating by making use of EPCD technology. The corrosion experiment of rich boron bioglass coating and plasma spray coating showed that split phase, rich boron and rich silicon phase, occurred during its preparation. In basic medium the corrosion behavior of 〓 BG coating showed uniformity corrosion, the corrosion mostly occurred at rich boron phase area, therefore batch formula design of BGshould avoid the occurring of split phase. The corrosion appearance of plasma spray coatingappeared a non-uniform corrosion, mostly occurred at the edge of the laminated HA moltendrops, and emerged an accelerated corrosion tendency, which will easyly lead to corrosioncrackles extending to the interface and the happening of osmotic interfacial corrosion, thatmay be one of the major reasons leading to the coating cracking-off in the later period. Thetesting results of thermal expansion coefficient of 〓 and 〓BG showed the thermalexpansion coefficient of 〓 BG matched with that of titanium alloy better, and 〓 BG couldsinter with titanium alloy into densification enamel layer at low temperature (720℃).

将Ti6Al4V合金在1000℃下进行真空热处理会降低其力学性能，且合金内的V元素会向表面富集，因此，钛合金真空热处理和表面涂层的烧结温度不能过高，即应低于其相转变点；通过对生物活性梯度涂层的组成和结构的设计，分析和计算了梯度涂层内的应力大小和分布，结果表明：对于本研究，当成分分布系数n=1.5时，可以获得较合理的涂层力学性能，即在沉积开始时，采用富含BG颗粒的悬浮液，以便在钛合金侧获得同底层BG有良好过渡的富BG涂层；梯度涂层界面和表面的应力大小、性质由材料组成本身的性质决定，改变成分分布系数，只能改变涂层内应力变化的趋势；涂层的厚薄不影响涂层内的应力分布规律，但涂层越薄，涂层内的应力变化越快，但这并不意味着涂层越厚越好，因为涂层越厚，涂层允许的变形越小，对于应用于弯曲受力部位的涂层而言，涂层应薄一点为好(50μm)；而对于仅纯受剪切应力的部位，如牙根种植体，涂层可适当加厚(80～100μm)；通过对HA和BG颗粒在水溶液体系和非水溶液体系中的带电特性和电泳沉积的研究发现，它们在水溶液体系中很难发生电沉积；在无水乙醇溶液中，HA颗粒带正电，可在阴极钛合金片上发生均匀的电泳沉积，而BG颗粒则带负电荷；利用复分解反应法，可以制得100～300nm的HA，通过诱导HA在BG颗粒表面结晶，可对BG颗粒进行表面包覆，获得了被HA包覆的BG颗粒，改变了BG颗粒表面的带电特性，使BG和HA颗粒在无水乙醇中均带上正电荷，从而实现了HA和BG颗粒在阴极上的共沉积。

In most simian infections, this does not happen because nef keeps the TCR-CD3 level too low for this exaggerated response to occur.

在大多数猿免疫缺陷病毒中，上述情形没有发生，因为nef基因将TCR-CD3蛋白控制在非常低的水平使得这种过度反应免于发生。

The chemical properties of gold ink too with acids, alkalis and sulfide contact occur to varying degrees, and that its metallic luster greatly reduced and Kim monlchamus becomes nigrescent, and second, it's easy to gold ink also with air, caused by water vapor chromotropic reactions, so that when you save the Gold Ink containers need to be tightened.

金墨的化学性质不够稳定，跟酸、碱和硫化物接触会发生不同程度的化学变化，而使它的金属光泽性大为降低，金墨就会显得发黑；其次，金墨还很容易跟空气、水蒸气发生化学反应而引起变色，因此，在保存金墨时一定要将容器密封盖严。

As the material is deformed beyond the strain that elastic deformation persists, the stress is no longer proportional to strain, and permanent, nonrecoverable, or plastic deformation occurs.

当材料的形变超出弹性形变发生的范围，其应力将不再与应变成正比，永久的、不可回复的形变发生，即为塑性形变。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。