将

Customer agrees to pay MCT upon demand such charges, fees and costs as MCT may from time to time charge (including but not limited to, markups, spreads, brokerage charges, commissions, service fees, transfer fees and other charges); all applicable regulatory and self-regulatory fees or charges, if any;any applicable tax imposed on transactions in foreign currency contracts;all compensation of any introducing broker or trading advisor payable from the Account, which MCT shall promptly deduct from the Account and remit to such person;the amount of any trading losses in the Account;any debit balance or deficiency in the Account;interest on any debit balances or deficiencies in the Account, at the rate set forth in this Section3, together with costs and reasonable attorneys' fees incurred in collecting any such debit balance or deficiency; andany other amounts owed by Customer to MCT with respect to the Account or any transactions therein. All commissions, charges or fees of MCT or compensation to any introducing broker or trading advisor shall be set forth on the Fee Schedule monthly e-mailed.

顾客同意根据MCT要求支付：MCT一直收取的各种费用(包括但不只是，提价，点差，经纪人费用，佣金，服务费，调整费用和其他费用)如有可能使用的管理或自我调整的费用在外汇交易合约中的可能交易税费任何经纪人或者投资顾问的所有费用都将从账户中支付，MCT将及时从账户中扣除并支付给他们；因账户中的交易失败；账户中出现的借方差额或资金不足；账户中出现的借方差额或资金不足而产生的利息，或如第三节所述连同代理人合理的费用和经费都将一起从账户中扣除；客户在账户中或交易中对MCT的所有其他欠款；MCT的所有佣金，经费，费用以及经纪人或投资顾问的酬金都将直接设置并每月发送月结报告邮件。

Customer agrees to pay JST upon demand such charges, fees and costs as JST may from time to time charge (including but not limited to, markups, spreads, brokerage charges, commissions, service fees, transfer fees and other charges); all applicable regulatoryand self-regulatory fees or charges, if any; any applicable tax imposed on transactions in foreign currency contracts; all compensation of any introducing broker or trading advisor payable from the Account, which JST shall promptly deduct from the Account and remit to such person; the amountof any trading losses in the Account; any debit balance or deficiency in the Account; interest on any debit balances or deficiencies in the Account, at the rate set forth in this Section 3, together with costs and reasonable attorneys' fees incurred in collecting any such debit balance or deficiency;and any other amounts owed by Customer to JST with respect to the Account or any transactions therein. All commissions, charges or fees of JST or compensation to any introducing broker or trading advisor shall be set forth on the Fee Schedule monthly e-mailed.

顾客同意根据JST要求支付：JST一直收取的各种费用（包括但不只是，提价、点差、经纪人费用、佣金、服务费、调整费用和其他费用）如有可能使用的管理或自我调整的费用在外汇交易合约中的可能交易税费任何经纪人或者投资顾问的所有费用都将从账户中支付，JST将及时从账户中扣除并支付给他们；因账户中的交易失败；账户中出现的借方差额或资金不足；账户中出现的借方差额或资金不足而产生的利息，或如第三节所述连同代理人合理的费用和经费都将一起从账户中扣除；客户在账户中或交易中对JST的所有其他欠款；JST的所有佣金、经费、费用以及经纪人或投资顾问的酬金都将直接设置并每月发送月结报告邮件。

Customer agrees to pay MCT upon demand such charges, fees and costs as MCT may from time to time charge (including but not limited to, markups, spreads, brokerage charges, commissions, service fees, transfer fees and other charges); all applicable regulatoryand self-regulatory fees or charges, if any; any applicable tax imposed on transactions in foreign currency contracts; all compensation of any introducing broker or trading advisor payable from the Account, which MCT shall promptly deduct from the Account and remit to such person; the amountof any trading losses in the Account; any debit balance or deficiency in the Account; interest on any debit balances or deficiencies in the Account, at the rate set forth in this Section 3, together with costs and reasonable attorneys' fees incurred in collecting any such debit balance or deficiency;and any other amounts owed by Customer to MCT with respect to the Account or any transactions therein. All commissions, charges or fees of MCT or compensation to any introducing broker or trading advisor shall be set forth on the Fee Schedule monthly e-mailed.

顾客同意根据MCT要求支付：MCT一直收取的各种费用（包括但不只是，提价、点差、经纪人费用、佣金、服务费、调整费用和其他费用）如有可能使用的管理或自我调整的费用在外汇交易合约中的可能交易税费任何经纪人或者投资顾问的所有费用都将从账户中支付，MCT将及时从账户中扣除并支付给他们；因账户中的交易失败；账户中出现的借方差额或资金不足；账户中出现的借方差额或资金不足而产生的利息，或如第三节所述连同代理人合理的费用和经费都将一起从账户中扣除；客户在账户中或交易中对MCT的所有其他欠款；MCT的所有佣金、经费、费用以及经纪人或投资顾问的酬金都将直接设置并每月发送月结报告邮件。

Cut off the power supply of the accumulator before installation, remove the former top light, pull out the original electric line with care while confirming the function of each hole of the base board, fix the base board with screw (better use the original top light screw), connect the down-lead of the product and bind to up, stuff the leftover back into the top shed, in sert firmly the main body, set the glider and fix it.

安装前必须先断开蓄电池的电源，然后拆下原车顶灯，小心拉出原电线，同时确认每条线的功能，参考上图，将原车软线穿过基座板中心孔，并用螺丝将本品引线相应接上并包扎好，然后将多余的引线塞回顶棚，同时再将主体牢牢地嵌入，滑动镶好进行固定。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Before the game, Kersey and other Trail Blazers alumni will join some of Duckworth's friends and family on the Rose Garden concourses, where they will sell Duckworth wristbands and "00" jersey patches to help raise money for the Kevin Duckworth Memorial Scholarship Fund.

达克的前队友和好友Jerom Kersey将主持仪式，届时他们将会在玫瑰花园球馆出售一些达克沃斯护腕和&00&号贴布，此外球馆通道内还将举行一个沉默式的拍卖，商品包括一个开拓者队的签名篮球，以及来自罗伊，阿德祖里奇，奥登的一些物品，所得收入将捐献给达克沃斯奖学金基金会。

The umbilical cord blood solution, which the DMSO concentration is 1.0M, 1.5M and 1.75M,was confected. The solution was in equilibrium for 20 minutes. Then, the umbilical cord blood was completely immerged in liquid nitrogen for about 24 hours. Then, the umbilical cord blood was taken out and was warming in the thermostat water bath for 20 minutes. The fraction surviving of the umbilical cord blood was more than 80%.

先将含有细胞的培养液、0.9％的NaCl和2.0M的DMSO溶液配制成分别DMSO浓度为1.0M、1.5M、1.75M的脐带血干细胞溶液，将配好的玻璃化溶液置于4℃静置20分钟使脐带血干细胞的玻璃化溶液达到细胞内外溶液平衡，然后将其投入液氮中大约24小时左右，最后将液氮中的细胞取出37℃水浴复温，细胞存活率达到了80％以上。

It is for this reason that we invite ascending initiates to put their minerals and mineral jewelry in water and in the sun for one to three hours with the intention to reunite them to our kingdom; in so doing, we will deprogram your minerals of foreign energy flow that is non-supportive of ascension and begin to reunite them into an overall flow that is associated with all minerals global wide.

正是这一原因，我们邀请每个提升者，将矿石河矿石珠宝放入水中，在太阳直射下停留1－3个小时，并意想将它们重新整合到我们的王国。这么做，我们将重新编程你矿石中不支持提升的外来能量流，开始将它们重整到和全球矿石相关的整体能量流中。

It is for this reason that we invite ascending initiates to put their minerals and mineral jewelry in water and in the sun for one to three hours with the intention to reunite them to our kingdom; in so doing, we will deprogram your minerals of foreign energy flow that is non-supportive of ascension and begin to reunite them into an overall flow that is associated with all minerals global wide.

正是这一原因，我们邀请每个提升者将矿石与矿石珠宝放入水中，在太阳直射下停留1－3个小时，并意想将它们重新整合到我们的王国。这么做，我们将重新编程你矿石中不支持提升的外来能量流，开始将它们重整到与全球矿石相关的整体能量流中。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。