封闭

The educational career that closes ten years, make they more or less the acerbity corrupt that was infected with some of bookman, be ashamed at talking about money, avoid at making money, also do not know how to make money.

十几年封闭的教育生涯，使他们或多或少的沾染了些文人的酸腐，羞于谈钱，避于赚钱，也不知道如何赚钱。

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

If the first group of curves you select form a closed boundary, the surface will be trimmed to that boundary.

如果你选择的第一组曲线来自一个封闭的边界，生成的曲面将被这个边界修剪。

A modefied egg function has been proposed and used in a bounding surface model. This model has potential value of applicaton since it reflects better actual situation of soil mass under loading. That is: when a stress point is still within the yield surface, the unrccovered plastic deformation would occur as stresses change, and using closed smooth function makes stress modification in the FEM much easier.

文中提出了一个改进蛋形函数，并采用这个函数建立了边界面模型，该模型由于机理上更好地反映了土体受荷时的实际情况，即当应力点在屈服面内时，随着应力状态的改变，仍然可以产生不可恢复的塑性变形，而在计算功能上由于使用了封闭光滑的边界面，不存在角点问题使有限元程序计算显得很方便，从这两个方面看，该模型具有潜在的应用价值。

The pendant is secured to a tapered single bale and presented on an 18-inch sterling silver box chain with a spring ring closure.

该挂件是固定在一个锥形单捆和一个18英寸的纯银弹簧圈封闭框链提出。

Zonal closure system allows for independent adjustment of the toe box to transition between hike and climb modes.

双分区封闭系统，配备可独立调整的趾头套，随时在登山和徒步两种模式中调整

An anti-lock brake system is a computer-controlled brake system that helps prevent wheel lockup during braking.

一个反封闭的制动系统是帮助防止轮子锁住在刹车期间的一个计算机控制的制动系统。

Congenital Preauricular Fistula (MIM 128700) is a common auricular abnormality, the incidence of which is around 1%. The formation of CPF is closely associated with embryological development of the auricle, which is developed from the first and second branchial arches.

先天性耳前瘘管（MIM 128700）是耳鼻咽喉科常见的遗传性疾病，其患病率接近1％，一般认为是胚胎期形成耳廓的第一、第二腮弓的六个小丘样结节融合不良或第一腮沟封闭不全所致。

Congenital Preauricular Fistula (MIM 128700) is a common auricularabnormality, the incidence of which is around 1%. The formation of CPFis closely associated with embryological development of the auricle,which is developed from the first and second branchial arches.

先天性耳前瘘管(MIM 128700)是耳鼻咽喉科常见的遗传性疾病，其患病率接近 1%，一般认为是胚胎期形成耳廓的第一、第二腮弓的六个小丘样结节融合不良或第一腮沟封闭不全所致。

You are a breach that must be closed.

你是一个必须封闭的裂口。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！