导入

So it can be essential momitoringindex of colon carcinoma and its tumor specificness is apply by manygene therapy of tumor. Transcriptional regulatory element of CEAcontains enhancer element and promotor in the 5th flanking region whichcan control expression of objective gene in positive cancer tissue of CEA. Objectives: To explore the joint promoter with high activity,consisting CEA promotor and CMV enhanser.

目的：本课题探讨由CEA启动子、CMV增强子组成的高活性嵌合启动子，通过融合启动子的调控提高融合自杀基因yCDglyTK在靶细胞表达的特异性；并以无毒副作用生物相容性好的磷酸钙纳米为载体，将该自杀基因靶向导入结肠癌细胞中，以达到提高基因治疗靶向性及安全性的目的。

Analysis on quantity of dsRNA, we injected the dsRNA of 5ul of 0.12ug/ul solution in spermatotheca of the female moth.

由下 dsRNA介导的基因干涉是属于转录后基因沉默，dsRNA导入后导致细胞中同源mRNA降解，从而干扰其内源基因的表达。

After that , simulated the Die Casting filling process of liquid metal .First use the spical soft Pro/E to construct the model of Die Casting part; Input it to another soft I-deas to form mesh and put the mesh document to the special soft ProCAST.

接着模拟了金属液在模具型腔的充填过程：运用Pro/E软件对泵盖进行三维实体造型，然后把图形导入软件I-deas中进行网格化处理，并将网格化后的模型输入到ProCAST软件中进行了关于压铸工艺参数的多组正交试验的流场，温度场的偶合模拟，进而从多组试验参数中找出最优的压铸工艺参数。

Attributed to the spiro structure, the polymer exhibited a very good thermal stability and suppress the formation of aggregating/excimers.

螺结结构的导入，使PTF具有良好的热稳定性，且有效抑制分子堆叠与激发复合体的产生。

At Ford it was the Edsel, a clunker-car with contrasting tail-fins and unlovely squarish styling, which sold only 68,045 in 1958, its first year, and 47,496 in its second, until he killed it in favour of the smaller, cheaper Falcon. At the Pentagon, where he arrived in 1961 with 99 topics for evaluation, he found a budget of $55 billion that had to be trimmed by bringing in systems analysis and five-year plans.

在福特，他栽在了埃德塞尔——这尾部扎眼，四方格调令人生厌的破车——之上，1958年首次推出销量仅为68，045，第二年也更是惨淡到了47，496；直到更经济更小巧的&苍鹰&问世，他才封杀埃德塞尔。1961年他进驻国防部，面对99个需要尚待商讨的问题，却发现只能通过导入系统分析，实行五年计划加以缩减，预算才能回顾550亿美元之水准。

Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.

构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体，将其导入逆转录病毒包装细胞PA317，通过G418抗性筛选，得到携带目的基因的包装细胞并鉴定；应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞，观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响；建立C6胶质瘤大鼠脑内移植动物模型，将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中，观察其治疗作用，并初步探讨其机制。

Results: The endoscopic system was slid through the incision and into the subpectoral space after the initial blind dissection in endoscopic-assisted group, and there were problems in 29 breasts of 22 patients (active bleeding in 28 breasts, residual fiber-bands in 25 breasts, hematoma in 13 breasts ), and were dealt with(hemostasis via endoscope, cutting off fiber-bands, clearing hematoma); there was no problem to be dealt with via endoscope, and in other 14 breasts.

两组略做比较，详细描述镜下所见及操作方法。结果内镜组盲剥后导入内镜系统，发现有29侧（分属全部22例患者）存在问题（活动性出血28侧，残余条索25侧，凝血块堆积13侧），进行了相应处理（内镜下止血、切断条索、清除血肿）；其余14侧未发现任何需要内镜下处理的问题。

To " home products wall " develop pattern, cheng Liang has not thought very clearly now, current pattern is the brand enumerate enterprise of each big home products is on home page, the autograph having reward that the person that browse can be invited to participate in real name to make after click supports, be guided " stagnant " the corresponding brand of forum discusses a subprovincial administrative region in.

对于&国货墙&的发展模式，程亮现在还没想得很明白，目前的模式是将各大国货企业的商标罗列在首页上，浏览者在点击后会被邀请参与实名制的有奖签名支持，并被导入&不景气&论坛的相应品牌讨论专区中。

Mc Donald' s is edier business leading CI strategy in USA. It' s"QSCD"is very famous succeedable foetor. MCDonald 's finally establish itseef in an anassaitable position and be came magnate in the wold fast food.

麦当劳是美国比较早的导入CI战略的企业，它的理念&QSCV&是它成功的一个重要因素，它使麦当劳在激烈的竞争中始终立于不败之地，跻身于世界强手之林，成为世界快餐业的巨子。

Traditional summative assessment system,which holds a dominant position in Chinese universities,demotivates autonomous learning.

本文探讨形成性评价在自主学习过程中的重要性，提出系统性导入动态形成性自我评价

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。