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We also confirmed in this paper that it is injected somatic cells but not broken cytoplasm formed multi blastomere through mitosis in the reconstructed embryos.

在本实验中还通过用DNA染料(Hoechst-33342)对核移植体进行染色的方法,对融合后成纤维细胞在核移植体内不同时间的运行及发育模式进行了观察,确认了是成纤维细胞经有丝分裂形成多个卵裂球而不是由于细胞质碎裂形成的两个或多个碎块。

Stem leaves in whorls of 4, shorter petiolate; leaf blade oblong, sometimes ovate, to 3 X 1.4(--2) cm, pinnatisect; segments ca. 10 pairs, lanceolate, pinnatipartite, dentate, teeth callose.

在4轮生,短叶柄里的茎生叶;叶片长圆形,有时卵形,对3 X 1.4(-2)厘米,羽状全裂;约的裂片10 对,披针形,羽状深裂,具牙齿,齿callose。

As we were getting the extra ,unseparated , duplet or splitted Peak in our analysis ( done in presence of Chinese people even with using their column and method ) these chromatograms are also exhibiting the unseparated, duplet,splited extra peak at the R.T. 2.42 and 2.54 having the equal basemarking width of only one peak compared to other peaks of the impurities.

初译:我们在分析时得到了额外的未分离的峰、对峰或裂峰(在中方人员面前当场试验,甚至使用中方人员的色谱柱和分析方法时也是如此),这些色谱图在保留时间为2.42和2.54分钟时也呈现出额外的未分离的峰、对峰和裂峰,与杂质的其它峰相比,这些峰的峰底宽不相上下。

Based on the analysis of sediments and diagenesis of YangXin Series, author studied systematically all types of holes and fractures including stracture fissures, secondary corrosion cavities, contract holes and crevices, unloaded fissures, regionalrifts crush cracks (mechanical, hydraulic and oil-gas divulsional), seperate fissures in layres, collapse breccia′s clinks and seam lines, etc,.

作者在对阳新统沉积及成岩后生普通化的分析基础上,全面系统地分析了包括构造裂缝在内的各种孔洞缝类型,如次生溶蚀孔洞、收缩成因孔缝、卸荷张缝、区域裂缝、压裂缝(机械压裂、水力压裂、油气扯裂)、层理脱空缝、垮塌角砾间孔缝、缝合线等。

Formation damage can be minored by using CO2 foam fracturing fluid as it has low filtration, high flowback and good compatibility with formation fluids.

由于CO2泡沫压裂液具有滤失量低、返排能力强、与地层流体配伍性良好等优点,采用CO2泡沫压裂技术,可减小压裂液对地层的伤害。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分为4个部分,第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究,确立了一种较好的培养方法:与颗粒细胞共培养,找到了一种适合猪卵母细胞体外成熟的培养基:mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸,成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%,国外报道的最高成熟率为(85.7±4.1)%;第二部分对猪卵母细胞孤雌激活的化学方法进行了研究,确立了化学激活猪卵母细胞的两种最佳方法:1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min,再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中,卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min,再与8mM的DTT共孵育30min,卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%;第三部分对孤雌激活胚胎的培养条件进行了研究,确立了一种最佳的胚胎培养条件:在SOF简单培养基中添加颗粒细胞进行前3天的培养,然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养,其桑椹胚和囊胚的发育率为(59.5±3.2)%;第四部分研究了IGF-I

Acidizing and fracturing are performed on perforated Berea sandstone targets after perforating tests in labs.

在实验室条件下对贝雷砂岩进行射孔试验,并分别对射孔后的靶体进行酸化、压裂工艺处理,利用核磁共振和工业X-CT技术分别对未射孔的靶体、射孔后的靶体、射孔后酸化的靶体、射孔后压裂的靶体进行测试。

The paper relying on project practice of the express highway from Taiyuan to Changzhi of Shanxi Province, according to demanding of "Technical Specifications for Construction of Highway Asphait Pavements ", combining with own region climate characteristic and concrete design, and construction request, it has carried out SBS modified studying on shell-90# asphalt, so drawing a conclusion: 1. Having studied the quantity of SBS modifier"s effect on the temperature sensibility, the high temperature stability, the low temperature resistance to cracking , elasticity and the durability of asphalt ,meanwhile, having studied the quantity of SBS modifier"s effect on the water stability, the high temperature stability , the low temperature resistance to cracking of asphalt mixture, having compared SBS modified bitumens various function. As a result ,when the amount of SBS added is 5%, every function is comparatively even.

本论文依托山西省太—长高速公路建设的工程实践,按照《沥青路面施工技术规范》的要求,结合本地区的气候特点和具体设计、施工要求,对壳牌90#沥青进行了SBS改性研究,得到如下结论:(1)研究了SBS改性剂用量对沥青的温度敏感性、高温稳定性、低温抗裂性、弹性及耐久性的影响,同时研究了改性剂用量对沥青混合料的高温稳定性、低温抗裂性和水稳定性的影响,比较了SBS改性沥青的各项性能,结果表明当SBS掺加量为5%时,各性能较为均衡。

Pombes growth inhibition experiment. The difference has statistic significance (P<0.01) between these cell treated with 5.0μM, 10.0μM and 20.0μM edelfosine for 6 h and those cell treated with 0μM edelfosine for 6 h.

利用粟酒裂殖酵母细胞胞核分裂试验和粟酒裂殖酵母DNA含量检测试验,观察依地福新对粟酒裂殖酵母细胞胞核分裂的影响。

Through statistic and analysis of interrelated data the effect rules of chemical composition,using frequenoy of mould,basic capacity of protecting slag,plate thickness,charging mode and cooling strength,e tc on star-like crack of plate were found.

通过相关数据的统计分析,找出了化学成分、结晶器使用次数、保护渣碱度、钢板厚度、装炉方式、冷却强度等对钢板显裂的影响规律;分析显裂产生的原因是,Cu原子、硅酸盐类夹杂物富集在奥氏体晶界,降低晶界强度,诱发显裂。

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