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Schistosoma japonica antigen cDNA clones were identified by lysogenic expression,flat lyiic method and PCR amplification. All 8 positive clones immunologically screened could be expressed in E- coli in the form of fusion proteins with the molecular weight being about 140 to 150 kDa. The positive cDNA genes were digested by restriction endonuclease EcoRI,then the agarose gel electrophoresis revealed the size of them being 700 to 900 bp.

利用融源表达、平板裂解法和PCR扩增三种不同方法分别对日本血吸虫抗原cDNA基因进行鉴定和分析,8个免疫筛选阳性克隆均能在大肠杆菌中以融合蛋白的形式表达,表达蛋白分子量为140~150kDa,抗原cDNA基因经限制性内切酶EcoRI酶解后,琼脂糖凝胶电泳显示其大小为700~900bp,PCR能扩增出特异性条带。

Characterization of the requirement of acetylation on histone H3 and histone H4 for EBV lytic DNA replication

鉴析组蛋白H3与组蛋白H4的乙醯化程度对於EB病毒溶裂期DNA复制的重要性

Just check blood alpha-fetoprotein in pregnant women, and found that neural tube defects (such as hydrocephalus, cerebral meningocele, spina bifida, microcephaly, no brains abuse, etc.) and some role.

只是孕妇抽血查甲胎蛋白,对发现神经管畸形(如脑积水、脑脊膜膨出,脊柱裂、小头畸形、无脑儿等)有些作用。

For some rock slopes, which have not distinct failure surface, but have complicated structure or load on the top and so on, a stability analysis of this type slopes by finite element method is presented, the failure surface is defined by certain rule or strain zone from the computed model, and then the safety factor of the rock slope is abtained by finite element method and limit equilibrium methodAt last, using ANSYS , a famous commercial finite element analysis software, a example of high-steep slope which is bearing the load of the LongGe Mansion is presented, the stability of this slope is studied, as a paradign the the stability evalution of complicated high-steep slope under the action of load of building , the chapter gives some meritable reference in stability assessment of rock slopes using numerical simulation by three-dimension elastic-plastic finite element.

并用有限单元法建立边坡的具体模型,应用折减边坡岩体参数、逐级施加荷载等与边坡工作环境相似的方法进行模拟计算,根据计算结果,采用一定的判别准则或图形可视化方法,确定出边坡的滑裂面,再采用刚体极限平衡法或有限单元法计算其安全系数;最后,通过某一工程实例,用大型有限元程序对边坡在建筑物荷载作用下的稳定性进行分析研究,并以此作为计算分析复杂高边坡在高层建筑物作用下的稳定性评价的一个范例,在采用有限元弹塑性数值模拟岩质边坡稳定性方面提供一些有价值的参考。

It was analyzed by means of metallographical examination, chemical composition analysis and inclusion testing, the results show that the fracture was mainly caused by no-metallic inclusion.

通过对层裂坯料进行宏、微观检验、化学成分和夹杂物分析,认为大颗粒硫化物夹杂及其富集破坏了基体的连续性,是镦制过程中产生裂纹的主要原因。

The level of metamorphopsia declined as a function of eccentricity and affected the central 10° of visual field.

黄斑裂孔的大小对眼间成像差异具有独立的影响作用。

We examined the response of human cord blood CD34+ cells to a specific mitotic signal in io.

我们通过动物体内实验检测人脐血CD34+细胞对特异丝裂信号的反应。

Hepatocyte growth factor/scatter factor is a multifunctional cytokine with mitogenic, motogenic, and morphogenic activities for renal tubular cells.

肝细胞生长因子是一个对肾小管上皮细胞具有强烈丝裂原活性以及细胞运动与形态调节的多效性细胞因子。

By the odd variance analysis to the average of AEMG, MPF and slope of MPF in the preliminary 20 seconds, we find that the falling slopes of MPF of the left multifidus muscle and the right multifidus muscle are remarkably influenced by driving time, not so other muscles.

通过对前20秒的AEMG、MPF的均数以及MPF的斜率进行单因素方差分析,左、右侧多裂肌前20秒sEMG信号特征指标MPF的下降斜率受驾驶时间影响显著,而其它肌肉的影响不明显。

4On the condition of unilateral holding load and enough high load levels, the activation pattern of the two lumbar muscles existed contralateral enhanced effect;(5)The back weigh loading had symmetrical effect for lumbar multifidus and erector spinae.(6)The left and right hand weight loading had the same effect in activation level; The lumbar muscles activation of went upstairs were higher than the plain and downstairs

在行走过程中,随着负荷等级的增加,竖脊肌收缩力增大,其AEMG值也相应增大;左手提与右手提对腰部两观察肌肉的激活程度具有同等效果;上楼时腰部竖脊肌、多裂肌的激活强度显著大于平地和下楼时的激活强度,而平地与下楼之间不存在差异。

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年美国个人破产法的一个改动使得破产登记急速下降,而后引起了信用卡大规模的亏损。

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弗四23 而在你们心思的灵里得以更新

Lao Qiu is the Chairman of China Qiuyang Translation Group and the head master of the Confucius School. He has committed himself to the research and promotion of the classics of China.

老秋先生为中国秋阳翻译集团的董事长和孔子商学院的院长,致力于国学的研究和推广。