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Objective To analyze humoral immune response to nucleic acid vaccines (VR1012/TPA/HG-MSP1-17 for intracellular expression or VR1012/HG-MSP1-17 for secretion) which contain Plasmodium falciparum Merozoite surface protein 1(MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT and to monitor the effect of DNA-immune sera on parasite inhibition.

摘 要:目的检测以恶性疟原虫裂殖子表面蛋白1的17区片段基因为基础的复合核酸疫苗(分泌性的VR1012/TPA/HG-MSP1-17和非分泌性的VR1012/HG-MSP1-17)诱导小鼠的体液免疫反应和免疫血清对疟原虫生长的抑制能力。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增,将扩增产物分别经EcoRI 和KpnI 双酶切后,回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体,按Sanger 双脱氧链终止法进行DNA 序列测定,并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

This article reviews the literature on microcrack s associated with ultrasonic root-end preparation.

然而,该技术也存在一定的局限性,学者们对超声牙根尖倒预备后牙根切面出现的微裂现象进行了广泛研究。

On the basis,the numerical response to micromechanics of material microst...

在此基础上,利用有限元法对异质体材料微结构进行细观力学响应的数值计算,对于识别"材料结构弱点",评估微裂纹的启裂、扩展,预测异质体材料微结构材料损伤后的材料性能,推演"微结构虚拟失效"行为具有重要的意义。

In clinic, orthognathic surgery such as LeFort Ⅰ osteotomy is a regular means to treat maxillary hypoplasia and severe midfacial discrepancy.

临床上,对严重骨性上颌后缩和唇腭裂引起的面中份发育不足,常采用外科正畸手段,即LeFort I型截骨前徙术进行治疗。

Leaves alternate,± sessile; stipules in pairs, small; leaf blade narrowly elliptic to almost needle-shaped, margin entire to incised.

叶互生,多少无梗;成对的托叶,小;叶片狭椭圆形到几乎的针形,边缘全缘到锐裂的。

Results Capillary has been found in the surrounding matrix before the neural canal closure.

结果 描述对脊髓血管胚胎各期的发育特征,分析脊髓纵裂畸形和椎体血管瘤伴发的脊髓动静脉畸形病例。

Pollcn morphology of 11 Heracleum species was examined under LM andSEM. It is shown that pollens of these specics are all of retangular type andequatorial constricted type, with polar views of 3-splitted round, obtuse triangle ortrianglc. Interspecific variations exhibit in pollen size and exine exolamelle.

对独活属11种植物的花粉进行光镜和扫描电镜观察发现,本属花粉为伞形科中比较进化的矩形型和赤道收缩型;极面观3裂圆形、钝三角形、三角形;花粉大小及外壁纹饰在种间存在明显差异。

Yes, it's right this Octagonal Pool.

韩佳:对啊,就是这个八角壁裂池。

Single leaf pinnate-wide crack on the health, lobes lanceolate, with serrated, upper leaves alternate from time to time, the oil gland lobes edge, serrated bearded, head-like inflorescence with Acremonium, diameter up to 10cm, yellow or orange, with a total pedicels swollen, flowering from August to September.

单叶羽状全裂对生,裂片披针形,具锯齿,上部叶时有互生,裂片边缘有油腺,锯齿有芒,头状花序着生枝顶,径可达10cm,黄或橙色,总花梗肿大,花期8-9月。

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