对照物

Methods The current conservative sequence primer and probe were designed according to the gene sequence of moderate and strong NDV in GeneBank. Fuorescence ration RT-PCR was done to the special TaqMan probe of moderate and strong NDV .The proliferation of NDV in chicken embryo was evaluated after taking Shuanghuang Shadu Tuishao Gule comparing with the control group.

根据GenBank登录的NDV中、强毒力株F基因序列，选择包含裂解位点的保守序列设计通用引物和针对中、强毒力NDV的特异性TaqMan探针进行实时荧光定量RT-PCR，评价双黄杀毒退烧颗粒干预后NDV强毒在鸡胚中和在机体内病毒的增殖情况，并与未干预的对照进行了比较。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

In the conditions of antagonistic mixture, Hyphal cell containing content changes nearely the contrast or has the same trend to a single partner over time.

而在拮抗组合条件下，菌丝细胞内含物含量随时间的变化与对照或某一单剂趋势相同。

Modified Taohong Siwu Decoction with Western medicine in treating traumatic hyphema 34 cases, as well as Western medicine treatment.

以加味桃红四物汤配合西药治疗外伤性前房积血34例，并设西药对照治疗。

At the depigmentation assay, DH and EH show dose-dependently inhibitory activities against mushroom tyrosinase, and the IC50 is 5.697 and 5.256 mM, respectively, and show noncompetitively inhibitory modes in the present of tyrosine, but not DOPA. The control synthetic products, D and E, have no inhibitory effects on tyrosinase. Using tyrosinase activity staining in PAGE gels, both DH and EH show dose-dependently inhibitory pattern in the presence of tyrosine, while, EH also could suppress tyrosinase activity in the presence of DOPA.

而美白活性方面，以商品酪胺酸以商品酪胺酸酵素进行实验，当基质为酪胺酸时，发现DH与EH具浓度相关抑制效果，IC50分别为5.697与5.256 mM，且其相对於基质酪胺酸之抑制型态皆为非竞争型抑制，而其结构类似物之对照化合物D与E则不具此活性；但当基质为多巴时则皆不具此活性；利用原态PAGE电泳活性染色亦发现抑制酪胺酸酵素结果，但EH在基质为多巴时也能下降酪胺酸酵素活性。

The optimal boron concentration for growth of pea (Pisum sativum L.) andcucumber (Cucumis Sativus L.) plants was screened by designing a series of boronconcentration in nutrient solution and the influeuce of boron deficiency or toxicityon integrity of cell membrane in different organs of the two plants was studied bymeasuring pH of the nutrient solution,〓 concentration and conductance in rootexudate solution and extracting solution of different organs from the two plants.

用不同浓度硼的营养液培养豌豆、黄瓜植株，筛选和确定在温室条件下生长所需的最适硼浓度和硼毒害浓度；进而通过对缺硼、对照和硼毒害处理后营养液pH变化，根分泌物和不同组织浸提液中K+浓度和电导率的测定，研究了缺硼和硼毒害对两种植物不同组织细胞膜完整性的影响。

In vivo the mixture of DBM/BMSC co-cultured for 3 days was planted into one side of muscles sacrospinalis of rabbits, and the DBM without BMSC was planted into other side as control.

将DBM和BMSC复合培养3d的复合物植入家兔一侧骶棘肌肌袋内，分别在1、2、4周活体取材，扫描电镜和苏木精-伊红染色后光镜观察，对侧单纯植入DBM作为对照。

The results indicated that with the increase of temperature and duration of treatment, the MDA content in rind increased significantly and the increase of soluble solids content, the decrease of titratable acid content in fruit and yellow stain of rind were inhibited, resulting in retarding the ripening process of fruits.

结果表明，与对照相比，温度升高或处理时间延长显著提高果皮MDA含量，抑制果实可溶性固形物含量的上升、可滴定酸的下降和果皮转黄，延缓果实的后熟过程。

The results showed that after exposure of germinating seeds to low temperature stress, the germination energy, germination percentage, germination index and seed vigor index in Zhengdan958 soaked with any of chemical regulation treatments under low temperature stress were higher than those of the unsoaked controls; In the meantime, the seedlings height, dry matter weight, leaf area, chlorophyll content of maize seedling under low temperature stress was elevated by A, B two kinds of concentration, indicating positive effect on strengthening maize seedling.

结果表明，低温逆境下各化控处理均提高了郑单958的种子发芽势、发芽率、发芽指数和种子简易活力指数；A、B两种药剂处理比对照都明显提高了低温胁迫下玉米幼苗的苗高、干物重、叶面积以及叶绿素含量，起到了良好的壮苗作用。

They therefore assessed small intestinal bacterial oergrowth by a direct test -- bacterial culture of small-bowel aspirates -- among 162 patients with IBS and 26 healthy controls.

于是研究人员使用了一种直接的方法——对小肠抽吸物进行细菌培养，以此来评估小肠内细菌过度生长。该项研究纳入了162名肠易激惹综合征患者以及作为对照的26名健康参与者。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。