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The result showed that the dosage of 5000 IJs/plant was the best one among the 8 dosages, the average of injured legume (3.l%) with BPS was significant lower than that of 40% isofenphos-methyl solution (9.0%) and than that of tap water (53.2%). The rate of grub decrease (95.7%) and the dry weight of peanut (5941.5 kg/ha) with BPS were significant different (P.05) to that of with 40% isofenphos-methyl solution (82.7%, 303.8 kg). The application of BPS was better with irrigation than with spray. At the dosage of 5000 IJs/plant, the combination of BPS and Rhabditis sp.(4500:500) did not show conflictive, coordinate or synergistic effect to grub control.

结果显示:在8个处理剂量中,以5000 IJs/穴的剂量田间效果最好,花生荚果受害率(3.1 %)显著低于40 %甲基异柳磷对照的(9.0 %)和清水对照的荚果受害率(53.2 %),蟒螬减退率(95.7%)和平均产量(5941.5 kg/hm^2)也与40%甲基异柳磷对照的(82.7%, 303.8 kg)有显著差异(P.05);灌施效果显著好于喷施;在5000 IJs/穴的剂量下,混合喷施BPs线虫和小杆线虫(4500:500),未表现出相克、协同或加性效应。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果:1、用PCR方法检测A组链球菌:以A组链球菌致热性外毒素基因speB为靶序列,设计的扩增引物对全部对照菌株的扩增结果为阴性,而全部A组链球菌参考株均能扩增出特异的345bp片段,其中包括三株猩红热链球菌,检测敏感性为6.5pg/μl DNA.2、用PCR方法检测白喉杆菌:以白喉外毒素基因toxB为靶序列,设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段,而全部对照株的扩增结果为阴性,检测敏感性为850fg/μl DNA.3、用PCR方法检测嗜肺军团菌:以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因,设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段,而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

Sugar and protein content in the haemolymph after fed with transgenic rice Total sugar content in the haemolymph of SSB fed with KMD1 was significantly lower than that of the control, while trehalase activity was significantly higher than that of the control, but there were no significant differences on trehalose concentration and glucose concentration between the treatment and the control. However, there were no significant differences on all the four components mentioned above between SSB fed on MSB and the control.

四、取食转基因抗虫水稻后二化螟幼虫血淋巴糖代谢和蛋白质浓度的变化五龄二化螟幼虫取食KMD1后血淋巴总糖含量显著低于取食秀水11对照的,海藻糖酶活性显著高于对照,而海藻糖含量和葡萄糖含量与对照组相比都无显著差异;取食MSB后二化螟血淋巴总糖含量、海藻糖含量、葡萄糖含量以及海藻糖酶活性与取食明恢86的对照相比,均差异不显著。

The degradation of DDTs fitted pseudo-first-order kinetics equation. The constants of degradation rate of DDTs between sterilized control and unsterilized control as well as between sterilized laccase addition and unsterilized laccase addition were close respectively, with the latter much higher than the former.

DDTs的降解过程符合拟表观一级动力学方程,非灭菌对照处理和灭菌对照处理之间,以及非灭菌加酶处理和灭菌加酶处理之间DDTs的降解速率常数分别相近,且加酶处理的降解速率常数明显高于对照处理。

This paper proceeded the flexural experiment respectively of two flexural strengthen beam by CFS, one compared reinforced concrete beam, and proceed sheared experiment research of two U style stirrups of CFS combined compacted sheared strengthen beam, one compared reinforced concrete beam. analyze and compare some working characters such as width of cracks, the deflection and ultimate moment of an unstrengthen compared beam, an initial strengthen beam and a beam strengthened after crack by compacting .

本文分别进行了2根粘贴碳纤维布受弯加固梁和1根钢筋混凝土对照梁的受弯试验研究及2根碳纤维布U形箍+压条受剪加固梁和1根钢筋混凝土对照梁的受剪试验研究,分析对比了对照梁、直接加固梁、压裂卸荷后再加固梁三者的挠度、裂缝、极限荷载等工作性能。

From seeding to florescence phase, water consumptions of potato of Wote15, Wote30, PAM15 and PAM30 were significantly lower than that in CK. From seeding to harvest phase, water consumption of potato of all treatments had no difference with that in CK. In florescence and harvest phase, water use efficiency and water output rate of Wote and PAM treatments were super significantly higher than that in CK.

从播种到花期沃特、PAM穴施用量15和30 kg/hm2处理的耗水量显著低于对照,从播种到收获期不同处理的耗水量与对照无显著差异,但花期、收获期不同处理的水分利用率和水分产出率均极显著高于对照

Seasonal variation of the distributions of microorganism in the root-zones of wetland plants (Phragmites australis, Zizania latifolia, Zizania latifolia etc.), the substrate layers of control SSPs, together with the effects of wastewater treatment were studied. The results are as follows: the numbers of microorganisms in the different plant root-zones were different; the purification effects were also different. The number of microorganisms in the substrate of the control plot becomes smaller with increasing depth. The numbers of bacteria in fall were higher than those in the summer for the three plant zones and the control, and the number of fungi and actinomyces in summer was much higher than that in the fall for control. As for fungi and actinomyces in the experimental systems, most of their difference between the summer and fall were not as big as that in the control.

通过对不同季节芦苇、茭白和香蒲等湿地系统植物根区、无植物对照基质微生物分布状况以及净化效果的研究,结果显示:(1)种植不同植物的湿地系统根区微生物数量不同,其湿地净化效果也不同;(2)对照系统微生物的数量随基质深度的增加而递减;(3)季节变化显示湿地系统秋季的细菌总数量比夏季高;但对照系统夏季的真菌和放线菌数量明显高于秋季;(4)湿地系统TP的去除率夏季高于秋季。

Methods The spontaneous contraction of gastric smooth muscle strip was recorded by using physiograph in rats. The change of tension in response to different concentrations of CNP and sodium nitroprusside, co-incubated with high concentration glucose (30 mmol/L) or mannitol were detected.

取大鼠40只制备胃窦环形肌条,肌条收缩活动稳定后,对照1、2组分别加入不同浓度CNP1×10^(-8、3×10^(-8)、1×10^(-7)mol/L及硝普钠1×10^(-7、3×10^(-7)、1×10^(-6)mol/L,观察其效应,每次加药观察20 min,冲洗3次;高糖1、2组预加葡萄糖使浴槽终浓度为30 mmol/L,其后操作同对照1、2组;甘露醇组预加甘露醇30mmol/L,其后操作同对照1组;均采用多道生理信号采集处理系统计算CNP致平滑肌张力的抑制率。

After treatment with chlorfluazuron to the larvae, the activities of POD in the larvae of three instars were all higher than the control, while the activities of POD in the second and third instar were only 65.0% and 99.2% of those in the control.

抑太保处理后,过氧化物酶活力除2龄幼虫在处理后5 h下降,仅为对照的65.0%,3龄幼虫在处理后1 h为对照的99.2%外,3个虫龄幼虫的POD活力均高于对照。H2O2由0增加至0.1 mL,POD活力呈线性关系上升。

Methods by using a broad pH range column(Gemini NX C18,4.6mm×150mm,5μm),basic mobile phase which was[H_2O-ammonia-glacial acetic acid] (96:3.6:0.4)-methanol,the related substances in etimicinsulfate were separated and detected by electrospray ionization and positive ion monitoring,the ESI ion source condition was following: temperature is 350℃,nebulizing pressure is 50 psi,dry gas flow is 10 L /min.some related substances in etimicinsulfate were identified by comparing the retention time in chromatography,~+ spectrum and MS~2 spectrum with reference substances\',the others which haven\'t reference substances were identified or speculated by analyzing their MS~2 fragmentation with the help of a rule summarized from the MS~2 fragmentation of gentamicin C1a,micronomicin and etimicin.Results nineteen related substances in etimicinsulfate were separated and detected.

方法利用宽pH范围的Gemini NX C18(4.6mm×150mm,5μm)色谱柱,在碱性条件下,以[水-氨水-冰醋酸(96:3.6:0.4)]-甲醇(70:30)为流动相,直接分离各组分;并用HPLC-ESI-MS~2检测样品中各组分,质谱条件:ESI离子源,离子源温度350□,雾化室压力50 psi,干燥气流速10 L/min,正离子检测方式;对有对照品的有关物质采用比对有关物质与对照品的色谱保留时间、准分子离子的质荷比及二级质谱方法进行鉴定,无对照品的有关物质,则是先通过解析庆大霉素Cla、小诺霉素、依替米星的二级质谱,对该类化合物的二级质谱裂解规律进行归纳,并以此推测未知新化合物进行结构,再用推测的结构对二级质谱进行解析,如所有的碎片离子均合理解释,表明推测合理。

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