对照...评价...

Objective To evaluate the clinical value of hemostatic scalpel in abdominal surgery.

目的 通过随机化对照研究，评价热凝切割刀应用于剖腹术的临床价值。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Objective To evaluate the role of histopathologic examine in cutaneous vasculitis.

目的：评价组织病理学在血管炎诊断中的价值。方法：本文对180例经组织病理诊断为皮肤血管炎的病例，进行系统的对照分析。

To evaluate the vascular response in relation to baseline, adjusting for systemic effects and skewed data, we expressed the hyperemic response as the natural logarithm of the ratio of postdeflation to baseline pulse amplitude in the hyperemic finger divided by the same ratio in the contralateral finger that served as control.

为了评价血管反应性与基线血管反应的关系，校正全身性作用及偏离的数据，我们计算袖套排气后充血手指脉搏幅度与基线脉搏幅度的比率以及对侧作对照手指的脉搏幅度与基线脉搏幅度的比率，两比率之比取自然对数表示充血反应。

Methods We electronically searched The Cochrane Library (Issue 4, 2009), PubMed (1980 to 2009), Ovid MEDLINE (1950 to 2009), Ovid EBM Database (1991 to 2009), EMbase (1980 to 2009), CBM (1978 to 2009) and CNKI (1979 to 2009) to collect literature about intravertebral analgesia for extern al cephalic version. We screened randomized cont rolled trials according to the predefined inclusion and exclusion criteria, extracted data and evaluated the quality of the included studies, and then performed meta-analyses by using RevMan 5.0.13 software.

计算机检索Cochrane Library （2009年第4期）、PubMed (1980~2009)、Ovid MEDLINE (1950~2009)、Ovid循证医学数据库(1991~2009)、EMbase (1980~2009)、CBM (1978~2009)和CNKI (1979~2009)，按纳入和排除标准筛选椎管内镇痛在外倒转胎位术中应用的随机对照试验，提取资料及评价文献质量后，采用RevMan5.0.13软件对数据进行Meta分析。

Methods 202 kinless subjects of the Chinese Han were investigated in this study, including 82 normal controls, and 120 cases diagnosed as CHD. The PPARδ+294T/C gene polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphisms. The frequencies of PPARδ+294T/C genotypes and the "C"allele frequency were analyzed, to evaluate the risk for the CHD among variant genotypes.

运用聚合酶链式反应及限制酶片段长度多态性技术分析无血缘关系汉族人群[包括82例正常对照者，120 例冠心病患者]的PPARδ+294T/C基因多态性，分析基因型频率、等位基因频率，并对不同基因型患者冠心病的危险性进行评价。

Appraised the clinical effect of the flavone of rhizome drynania on Senile Osteoporosis, according to the dynamic observation on integrative scores of lumbodorsal pain and lumbodynia,"VAS section grading method" on lumbodynia,"Barthel index rating method" on ADL, detected BMD on anterior-posterior position lumbar2~4 vertebra body and proximate of femur including neck/ward/troch, and determined the index of bone transition including BGP/PINP/ICTP through the radioimmunity method before and after treated half a year, then contrast intragroup and selfgroup. Depending on the standard of curative effect, contrast the difference of curative effect between two groups.

采用随机对照的方法，分为治疗组36例，对照组34例，依据对腰背痛及腰痛综合评分进行动态观察；依据&疼痛VAS线段分级法&对腰背痛（静止痛、翻身痛、久坐痛、起身痛、弯腰痛、步行痛、睡眠痛、叩击痛、压痛）进行量化动态观察；依据&Barthel指数评定法&对老年性骨质疏松症日常生活活动能力进行观察；采用双能X线骨密度仪对腰椎(L2-L4)、股骨近端各部位：股骨颈、股骨三角区、股骨粗隆进行骨密度定量检测；应用放射免疫法对骨转换指标进行测定，骨形成指标：骨钙素、Ⅰ型前胶原氨基端前肽；骨吸收指标：Ⅰ型胶原交联羧基末端肽等，评价骨碎补总黄酮治疗老年性骨质疏松症的临床效果。

Objective To evaluate the feasibility and efficacy of laparoscopic total mesorectal excision in treating middle and low rectal cancer.

目的：通过与开腹直肠全系膜切除的前瞻性非随机对照研究，评价腹腔镜TME治疗中低位直肠癌的临床疗效。

According to terrestrial heat professional in China Energy Sources Research Institute: the hot spring water temperature in Tangchi, Lujing County is 63℃ and is the typical hot spring water with sodium sulfide and the total minerals content is 1.2kg/Liter. Compared with "Medical Thermal Mineral Water Quality Standard "(refer to natioanl standard GB11615-89 ), the spring water in Tangchi belongs to the fluorin water and the silicion water of the thermal mineral water. It contains metaboric acid,lithium,sulfureted hydrogen etc. and has great effect on many diseases such as arthopathy, cardiovascular disease, digestive system diseases, skin diseases, and neuritis, respiratory infections, etc.

中国能源研究会地热专业文员会评价：庐江县汤池温泉水63℃，属于硫酸钠型水，是典型的温泉水质类型，其所含矿物质总量高达每升1.2克；对照《医疗热矿水水质标准》（见国标GB11615-89），汤池温泉属于医疗热矿水的氟水和硅水，并且其中所含的偏硼酸、锂和硫化氢等成分具有综合疗效，这种类型的医疗热矿水对骨关节病、心脏血管病、消化系统病、皮肤病、神经系统病和呼吸系统病等数十种病症，均能具有显著或有效的疗效。

Background: Currently, there are no published randomized controlled trials evaluating the efficacy and safety of adjunctive antiepileptic therapy in idiopathic generalized epilepsy with myoclonic seizures.

研究背景：最近没有评价辅助抗癫痫疗法对于特发性全身性癫痫伴肌阵挛性发作的效果和安全性的随机对照研究的文章发表。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。