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When the cells have adhered to coverslip after being cultured for 24h, we use 70% ethanol to fix the cells for 20 minutes standby.

CD133表达流式细胞仪分析:分析对数生长期的骨肉瘤细胞系Saos-2细胞的CD133表达百分率。

They were cut into 3μm standby. Check logarithmic growth phase Saos-2 cells subcultured in Petri dish with the coverslip, which has been sterilized.

取Saos-2细胞系对数增长期细胞,传代于放置有消毒后盖玻片的培养皿中,培养24h制备细胞爬片,使用70%乙醇固定20分钟备用。

The optimal conditions for electroporation transformation of Pichia Pastoris were obtained with the maximum transformation efficiency of 36 transformants/μg plasmid DNA when the intermediate logarithmic phase yeast cells were used for competent cells, and the electroporations were under the conditions of voltage of 1.5kV and 0.15mg / mL plasmid DNA and 0.2 cm cuvettes.

结果表明:当采用对数生长中期的菌体制备感受态细胞、电压为 1.5kV、质粒浓度为 0.15μg/μL和 0.2 cm 电转化杯时,转化效率达到最大值,为每微克质粒 DNA 36个转化子。

The results indicate, the δ^13C1 value becomes heavier gradually as coalbed methane desorbs. The δ^13C1 value and the desorption time displays a logarithm relationship.

结果表明,随着解吸过程的进行,δ^13C1值逐渐变重,δ^13C1值和解吸时间呈对数关系,δ^13C1值变重趋势具有先快后慢的阶段性特点。

Based on an analysis of 124 cases of family punishment, the paper argues that, expect for a small number of deviances, the collective death penalty was meted out basically to "parents, wife and off-springs" of the accused during the Qin and Han dynasties.

本文根据对数十件族刑案的分析,认为秦汉时期族诛的范围基本上是&父母妻子同产&,仅在特殊情况下有小范围伸缩:大约以宣元时期为界,族刑的施刑方式渐由诛杀向诛徙转变。

The spin-1/2 dimerized antiferromagnetic system is related to spin-Peierls tran-sition.There are still some discrepancy on the behavior of the ground energy densityand energy gap,such as pure power-law,logarithmic correction to the pure power-law in the uniform,integer powers in the dimerized limit,etc.

自旋S-1/2的二聚化反铁磁链与spin-Peierls相变有关,在对其基态能和激发能隙的临界行为描述上仍存在一些不完全一致的观点:如均匀极限的纯幂级数律,纯幂级数律的对数修正;二聚化极限的整数幂级数行为等。

The molecular extinction coefficient is equal to the ratio of the log of the intensity of the incident and emergent light.

分子的消光系数等于入射光和透射光强度对数的比。

The molecular extinction coefficient is equal to the ration of the log of the intensity of the incident and emergent light.

分子的消光系数等于入极大时的入射光和透射光强度比的对数

You can perform any of the standard operations for which you would use a calculator, including basic arithmetic, such as addition and subtraction, as well as functions found on a scientific calculator, such as logarithms and factorials.

您可以执行任何操作的标准,您将使用一个计算器,包括基本的算术,如加减,以及功能上发现一个科学计算器,如对数和阶乘。

In this paper,based on consulation to tens of bridge experts and a vast amount of investigation and study,expert knowledge on bridge assessment is obtained. A new concept-damage value is put forward to measure bridge damage,and fuzzy theory is used to create comprehensive fuzzy system to assess bridge service property. The set up of an expert system for bridge assessment is tried as well.

本文在对数10位桥梁专家的咨询以及大量的调查研究基础之上,收集整理了桥梁评估专家知识,提出用损伤度来度量桥梁结构或构件的损伤程度,并采用模糊数学原理,建立了桥梁使用性能的模糊综合评估体系,并探索建立了桥梁评估的专家系统。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?