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Results: It was observed that projections from IN to hypothalamus passed through the superior cerebellar peduncle, crossed in its decussation and then many of them entered posterior hypothalamic area by iontophoresis of Dextran-Texas Red to cerebellar IN. Using the methods of iontophoresis of Fluoro-Ruby and double labelling staining, it was also observed that the neurons projecting to PH in cerebellar IN were glutamatergic neurons.

结果:小脑间位核电泳Dextran-TR后示踪可见间位核神经元发出离核纤维,行走于同侧小脑上脚中,在小脑上脚交叉处交叉到对侧,然后继续走行于小脑上脚中,到下丘脑后较多纤维终止于下丘脑后区;在下丘脑后区电泳Fluoro-Ruby后可逆行追踪到小脑间位核神经元,荧光双标法显示投射到下丘脑后区的小脑间位核神经元中有谷氨酸能神经元。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造,构建截去AIF基因线粒体定位信号,FAD结合结构域和NADH结合结构域(1-400位氨基酸)编码序列的AIF△1-400基因,将其克隆入pcDNA3和pIRES2EGFP真核表达载体,用脂质体法转染HeLa细胞,通过荧光显微镜观察,MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造,构建截去AIF基因线粒体定位信号,FAD结合结构域和NADH结合结构域(1-400位氨基酸)编码序列的AIF△1-400基因,将其克隆入pcDNA3和pIRES2EGFP真核表达载体,用脂质体法转染HeLa细胞,通过荧光显微镜观察,MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

The author mainly realized the method of generating the large random numbers and judging the prime number using Lehmann means,then three pairs of secure key with different lengths (256 bits,512 bits,1024 bits) was generated to meet the demand of different security grade. In encryption module,modular multiplication arithmetic of large number was realized adopting addition chaining. In decryption module,extended Euclid was used to get inverse,and the Chinese remainder theorem was used to realize decryption,which increased the speed of decryption greatly.

作者主要实现了大随机数的生成、采用整除小素数和勒曼测试法判定素数,可以生成三种不同长度的密钥对(256位、512位、1024位)来满足不同的加密安全等级的需求;加密模块中,选用了二进制序列的乘法方法,实现了大数模幂乘运算;解密模块中,引用了扩展的欧几里德法求逆元,并采用中国剩余定理实现解密过程,大大提高了解密速度。

But a thicker cell is necessary to keep higher precision. Superimposing a constant phase difference proposed in the thesis make it is possible to measure the pretilt angle in thinner cell. This method was applied in our newly developed pretilt angle tester successfully.

晶体旋转法是测试预倾角的常用方法,但要求有较高的盒厚,本文提出的叠加一位相差的方案使这种方法可以实现对较薄盒的测试,并成功的应用于我们最新开发的预倾角测试仪。

Arethusa was once a fairy maiden huntress.she carriedbow and arrows for artemis in her hunting.she was sodevoted to her work that she cared for neither admirationnor love.one sunny summer day,she felt hot and found a coolstream quite attractive.she jumped into the delightful streamand happily started swimming.presently she heard a bubbling in the stream and was surprised to find it was the thunderingvoice of the rivergod alpheus.the maiden made for the shore and,fully naked,started running away.taking a human form,the river-god followed closely in pursuit .onward they spedover hill and valley,across dark heights and over broad plains,until the waters of the western sea lay stretched out right before them .helplessly the exhausted arethusa cried to her patronessfor help.artemis instantly cast a cloud over her body,but the pigheaded alpheus was not to be tricked.then a cold sweatbroke from the maiden''s arms and legs.the drops of water fellfrom all over her body.she had been turned into a spring!

阿瑞塞莎是位迷人的仙女猎人。每逢阿耳特弥斯打猎时,她负责携带弓箭。她对工作专心致志,既不考虑赞美也不顾及爱情。一个炎热的夏日,她感到很热,便找到一条凉爽的溪流,深感惬意。她跳入令人愉快的溪水中,兴致勃勃地畅游。不一会儿,听到水泡声,她大吃一惊地发现:那是河神发出的闷雷声。仙女向岸边游去,赤身裸体地跑开了。河神便装成凡人的样子在后紧紧地追赶着。他们一直向前飞奔,越过小山,跨过峡谷,翻越大山,掠过广阔的平原,直到西海的水域展现在他们的眼前。绝望中精疲力竭的阿瑞塞莎哭叫着向她的保护神求救。阿耳特弥斯迅速将一层云雾披在仙女的身上。但顽固不化的阿耳法斯并未上当受骗。接着一股冷汗从仙女的肢体上溢出,并且浑身上下淌着水珠,她被变成一眼泉水了。

Arethusa arethusa was once a fairy maiden huntress.she carriedbow and arrows for artemis in her hunting.she was sodevoted to her work that she cared for neither admirationnor love.one sunny summer day ,she felt hot and found a coolstream quite attractive.she jumped into the delightful streamand happily started swimming.presently she heard a bubbling in the stream and was surprised to find it was the thunderingvoice of the rivergod alpheus.the maiden made for the shore and ,fully naked ,started running away.taking a human form,the river-god followed closely in pursuit .onward they spedover hill and valley ,across dark heights and over broad plains, until the waters of the western sea lay stretched out right before them .helplessly the exhausted arethusa cried to her patronessfor help.artemis instantly cast a cloud over her body,but the pigheaded alpheus was not to be tricked.then a cold sweatbroke from the maiden''s arms and legs.the drops of water fellfrom all over her body.she had been turned into a spring!

阿瑞塞莎阿瑞塞莎是位迷人的仙女猎人。每逢阿耳特弥斯打猎时,她负责携带弓箭。她对工作专心致志,既不考虑赞美也不顾及爱情。一个炎热的夏日,她感到很热,便找到一条凉爽的溪流,深感惬意。她跳入令人愉快的溪水中,兴致勃勃地畅游。不一会儿,听到水泡声,她大吃一惊地发现:那是河神发出的闷雷声。仙女向岸边游去,赤身裸体地跑开了。河神便装成凡人的样子在后紧紧地追赶着。他们一直向前飞奔,越过小山,跨过峡谷,翻越大山,掠过广阔的平原,直到西海的水域展现在他们的眼前。绝望中精疲力竭的阿瑞塞莎哭叫着向她的保护神求救。阿耳特弥斯迅速将一层云雾披在仙女的身上。但顽固不化的阿耳法斯并未上当受骗。接着一股冷汗从仙女的肢体上溢出,并且浑身上下淌着水珠,她被变成一眼泉水了。

Quantum chemical calculations were performed based on these conformations to explain the experimental data. 4. The molecular recognition of the chiral Salen Fe complexes towards imidazole derivatives and the measure of the equilibrium constants of host-guest system of the Salen Fe and EMIm a.

用紫外-可见光谱滴定法考察了不同主体Salen-Fe对咪唑及其衍生物的分子识别行为,测定了识别过程的平衡常数和热力学参数,讨论了影响主客体配位能力的有关因素;系统地考察了主客体缔合反应的圆二色光谱性质。

This paper presents a rapid inversion method for 3D apparent density based on separation and continuation of potential field. The new method is carried out in the following three steps. Firstly, the gravity field on the plane is separated by a cutting method into several parts corresponding to strata of different depth. Secondly, by the way of downward continuation, the field resulted from different strata is respectively reduced to a plane corresponding to its depth.

文中提出了一种基于位场分离与延拓的视密度快速反演方法:首先应用场分离的切割法对平面上的重力场进行不同深度层源的切割分离;然后运用大深度向下延拓方法将各层的场延拓至相应的深度;最后反演出各深度层的密度。

Methods: Techniques of three dimension cursor based on volume multi-orientation sections were used to identify and localize precisely the cerebral central sulcus on the continuous coronal and sagittal MRI by using a person computer.

应用个人计算机设备,采用计算机辅助的三维定标法,对大脑冠状位、矢状位MRI上中央进行了识别、定位,并与连续追踪法结果比较。

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