对位法

Research on the Design Method for Restrainers Based on the factors effected the usage of restrainers, the design methods for the restrainers in use are analyzed, and a new design method is put forward based on response spectrum method. The method is tested with time history analysis. According to the design idea of unseating prevention devices, the static-based approach for restrainer devices is suggested.

5限位器设计方法研究在了解和分析影响限位器作用效果的各种因素的基础上，对现有限位器设计方法的优劣进行对比，提出了基于反应谱法的应用范围更广泛的限位器的实际计算方法，并采用非线性动力时程分析对计算结果进行了验证；参照连梁装置的设计思想，提出了常用的锚固限位器和限位挡块的静力设计方法。

It emphasizes some ordinances with more disputes but faultiness or losing in legislation, and make relevant suggestion: that the insurer exercising the right of subrogation should justify paying the insurance compensation; the insurance compensation of inadequate insurance should be distributed by proportion ,according to the proportion of insurance subject matter assumed by the insurer and the insured; the definite items of the staple of the insured"s synergic obligation , the cost of exercising the synergic obligation should be stipulated by the law; and if the insured doesn"t exercise synergic obligation, he or she should liable for relevant legal responsibility etc.

本文以我国保险代位权的立法现状为出发点，以民商法基本理论为立足点，结合海内外的最新研究成果，将理论研究与实践相结合，运用历史分析、比较分析与实证分析的研究方法，从法学、社会学角度进行分析研究，并取得了以下研究成果：论文构筑了一个对保险代位权研究的综合分析框架；指出保险立法不完善及某些方面冲突等原因，导致保险代位权在实践中未充分发挥作用；提出保险代位权的本质是法律给予保险人减少风险损失的手段，是对社会资源加以公平配置的方式；损害赔偿金在保险人和被保险人之间的分配原则：按照他们各自对保险标的承担的风险比例进行分配；指出在人身保险中不适用代位权以及保险人有权对公法人行使代位权等观点。

Effects of these three groups were evaluated with electrophysiological,histological and ultramicroscopic observations.

结果表明，纤维蛋白粘合剂、ZT胶的修复效果与缝合法无显著差异，并有简便，省时省力，无神经损伤，对位准确等优点，有较高的临床应用价值。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

A mixed linear model approach and its software were used to detect QTLs with main effect, QTLs involved in digenic interactions and Q×E interactions.

采用基于混合线性模型的复合区间作图法和相应的作图软件QTLmapper/V2.0，分别定位了7个株高和6个穗位高QTL；检测到18对控制株高和13对控制穗位高的上位性效应位点；同时发现了与环境存在显著互作的6个株高和8个穗位高单位点标记区域以及4对株高和4对穗位高上位性效应区域。

Methods Take Millard law as basis,piece of contrapuntal suture C fixed point being designed,rotating,full muscle of mouth wheel sneaking under the piece of skin parting for and will be located in nose pillaret,the wing of nose place in A,B is away from breaking,revolution cotton-padded mattress style sews the ridge taking form up,to be good for lip external shape formation and functional recovering.

以Millard法为基础，定点设计、旋转C瓣对位缝合，在A、B瓣皮下潜行分离并将位于鼻小柱、鼻翼处的口轮匝肌离断，旋转褥式缝合形成嵴，以利于唇外形的形成及功能的恢复。

After induced with IPTG, the proteins expressed by different fragments were analyzed by SDS-PAGE, and the results showed that all these truncated proteins could be expressed in E. coli BL21. Bioassay result against Plutella xylotella showed that the active fragment of Cry1Ba3 protein was located between amino acid No. 22 and No. 655. As the insecticidal activity of the truncated protein containing amino acid No. 54-655 was declined significantly, and No. 22-627 and No.

采用浸叶法检测这些蛋白片段对小菜蛾的杀虫活性，研究结果表明含有第1～685位和含有第22～655位氨基酸的蛋白片段对小菜蛾的毒性，与全长Cry1Ba3蛋白相比，没有改变；含有第54～655位氨基酸的蛋白片段对小菜蛾的毒力明显降低；而含有第22～627位和含有第85～655位氨基酸的蛋白片段完全丧失了对小菜蛾的活性。

In the emic approach, the goal is to describe behavior in one culture or ethnic group in terms that are meaningful and important to the people in that culture or ethnic group, without regard to other cultures or ethnic groups.

CET-6， 2002.6， Passage 1， Paragraph 2 着位法的目的是描述某一种文化或者一个种族中对其有意义或重要的行为，而不必涉及其他文化和种族。

Method］retrospectively analysis of 17 3 cases of femoral intertrochanteric factures treated with dhs with was reported in which 23 cases failed.

方法］回顾性分析173例采用dhs治疗股骨粗隆间骨折，其中内固定失败23例，运用logistic回归分析法分析6个可能影响dhs内固定失败的因素(性别、年龄、singh指数、损伤原因、garden对位指数、骨折类型)。

At the same time, the finite element model of six-phase ten-pole fault-tolerant permanent magnet machine is established to simulate and compute static and transient electromagnetic fields using vector magnetic potential method.

同时建立了六相十极永磁容错电机的有限元仿真模型，利用有限元矢量磁位法对该模型进行了静态和瞬态电磁场的仿真分析。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。