对...

Methods:(1) Silica column chromatography and LH-20 were used in phytochemistry study.(2) Based on the extraction ratio of arctiin, the best extraction procedure was acheived under the direction of L9(34) orthogonal experimental design. From 10 types of resins, the one with the maximal binding ability for Arctii was selected and silica-column choramatograpy was used in its seperation and purification procedure.(3) The animal diabetic experimental model in Wistar rats was established with streptozotocin and many biochemical factors were used to indicate the pharmacological effects of arctiin, including glycosylated hemoglobin, serum creatinine, seralbumin, monoxide nitrogen, glycerin trilaurate, cholesterol, HDL-ch, LDL-ch, Blood urea nitrogen, creatinine, SOD, ET, MDA, total Urine protein and albuminuria.

(1)采用硅胶柱层析、聚酰胺层析、LH-20等方法对牛蒡子的化学成分进行研究；(2)采用正交实验设计方法，以牛蒡子苷为指标，确定了牛蒡子乙醇回流提取的最佳工艺；对10种类型大孔吸附树脂进行筛选，筛选出对牛蒡子苷吸附效果最佳的树脂；采用硅胶柱层析法，对牛蒡子苷进一步分离纯化；(3)建立实验性糖尿病大鼠模型，以GLU、TC、TG、HDL-ch、LDL-ch、TB、ALB、BUN、CREA、GHbAlc、NO、SOD、ET、MDA和尿液中TB、ALB为指标，研究牛蒡子苷药理活性；(4)以差速消化法纯化Wistar大鼠的主动脉内皮细胞并进行传代培养，测定牛蒡子苷对高糖条件下RAECs活性、RAECs释放LDH、MDA和NO的变化以及RAECs表达内皮型一氧化氮合酶表达的影响。

The effects of concentration, time on the reaction was measured by UV-visible absorption spectra , the morphology of the silver nanoparticles was characterized by transmission electron microscopy; The structure of Sesbania Gum and silver colloid was measured by Fourier transform infrared spectroscopy .

通过紫外-可见吸收光谱研究了反应物浓度、反应时间对反应的影响；通过透射电子显微镜对银纳米粒子形貌进行表征；利用傅立叶变换红外光谱仪对田(来源：A116161BC论文网www.abclunwen.com)菁胶和反应所得到的银溶胶的结构进行表征；对溶胶的拉曼光谱进行检测，结果显示溶胶具有很好的Raman信号，室温下得到的溶胶表现出银纳米粒子对田菁胶的Raman信号增强作用。

Through simulation of the real process of thomcolumbar burst fracture and use of modem dynamics techniques, the present study was (1) to investigate the kinetic relationship between structural damage and impact energy absorption and dynamics mechanism of thoracolumbar burst fracture by quantitative analysis of various injured segments;(2) to explore the instability mechanism of L1 vertebral burst fracture and its injury threshold by three-dimensional analysis of the injured segment with stereophotogrammetry in combination of transient physical parameters, anatomy and image;(3) to explore the corresponding relationships between impact energy, geometry parameters and biomechanics by geometry and biomechanical analysis of thoracolumbar burst fracture, which may provide an objective standard for evaluation of spinal injury severity and experimental evidence for adoption of biomechanical treatment in clinic;(4) to evaluate the fixation effect of different instruments and the effect of intervertebral bone graft on segment fixation by analysis of the threedimensional stability of different internal fixation instruments and their decompression of the spinal canal, which may provide experimental evidence for therapeutical selection for thoracolumbar burst fracture in future; and finally to investigate the effect of various instrument fixation on dynamical characteristics by comparison and quantitative analysis of frequency and amplitude responses, which may provide certain theories and experimental evidence for application of vibration test to judge the stability of the spine.

本研究从胸腰段脊柱爆裂骨折发生的实际过程出发，以现代动力学检测及计算机多通道高性能数据采集分析系统等高新技术为基础，实时量化分析损伤节段的工程参数，揭示了结构破坏与能量吸收的变化规律，探讨了胸腰段脊柱爆裂骨折的瞬态损伤机制；利用三维立体摄像技术，对不同损伤程度的节段进行三维运动分析，结合瞬态物理参数、影像学和病理解剖，明确了〓椎体爆裂骨折的失稳机制及损伤阈值；通过对胸腰椎爆裂骨折的几何学变化及生物力学分析，明确了撞击能量与几何参数、几何参数与生物力学的相应关系，为评判脊柱损伤程度提供了客观标准，为治疗中采取适宜的生物力学方法提供了实验依据；通过对比分析不同内固定器械的三维稳定性和对椎管的减压作用，评价不同器械的复位固定作用，同时比较椎体间植骨对节段固定作用的影响，为今后临床胸腰段脊柱爆裂骨折的治疗选择提供依据；利用振动测试与分析技术，比较及量化分析了不同致伤状况的频幅响应特征，比较分析了各种器械固定对动力学特性的影响，为今后临床建立基于振动测试与分析技术判断脊柱稳定性的诊断方法，提供了一定的理论和实验依据。

We discuss the effects of GLJ about depressing the spasmodic ileum by experiments on isolated apparatus or holistic animal, in order to learn the mechanism and offer the data to exploiting and researching the new preparation.1 The action of GLJ on the intestinal smooth muscle of holistic animal.1.1 Observe the action of GLJ on normal intestinal propulsion of NIH rats and sthenic intestinal propulsion due to Neostigmine.

GLJ对在体动物肠平滑肌运动功能的影响 1.1观察GLJ对正常小鼠肠推进功能和对新斯的明所致推进功能亢进的小鼠的肠推进功能的影响，结果表明，50mg／kg、100mg／kg、200mg／kg的GLJ对正常小鼠肠推进功能均无显著影响，但对新斯的明所致推进亢进小鼠肠推进功能均有显著抑制作用。

Huapai products:pchlorophenol,ochlorophenol,2,4dichlorophenol,2,6dichlorophenol,2,4,5trichlorophenol,2,4,6trichlorophenol,4chloro2benzyl phenol,pbromophenol,2,4,6tribromophenol,1chloro4(1,1dimethyl ethyl) benzene ether,3bromoanisole,mbromofluorobenzene,2tetra hydrofuran,αbromo4chloro acetophenone,αbromo4methoxy acetophenone,4chloro benzophenone,4chloro2methylaniline,n(2hydroxyethyl)ethane diamine; n(2aminoethyl)ethanolamine,2,3dibromopropionyl chloride,4bromo benzene sulphonyl chloride,terephthaloyl chloride,isophthaloyl chloride,nchlorosuccinimide,nbromosuccinimide,pchloranil;tetrachloro1,4benzoquinone,5chloroisatin.

华派公司产品：对氯苯酚，邻氯苯酚，2，4二氯苯酚，2，6二氯苯酚，2，4，5三氯苯酚，2，4，6三氯苯酚，4氯2苄基苯酚，对溴苯酚，2，4，6三溴苯酚，对氯苯基叔丁基醚，间溴苯甲醚，间溴氟苯，α溴甲基四氢呋喃，α溴代4氯苯乙酮，α溴4甲氧基苯乙酮，4氯二苯甲酮，4氯2甲基苯胺，N(2羟乙基)乙二胺， 2，3二溴丙酰氯，对溴苯磺酰氯，对苯二甲酰氯，间苯二甲酰氯，N氯代丁二酰亚胺，N溴代丁二酰亚胺，四氯苯醌，5氯靛红。

According to the statistical analysis to cases of cerebral hemorrhage, and the research its pathology and physiology, we put forward an idea that operation is necessary to deal with cerebral hemorrhage, especially for hypertensive type. Moreover, the detailed description of methods of surgical treatment, and their summerize and mutual comparision which are being references in use for its clinical treatment.

本文根据对脑出血病例的统计学研究，以及对其病理生理的探索，提出了对脑出血，尤其对高血压性脑出血进行外科手术治疗的必要性，并且详细阐述了针对其外科手术治疗的方法，同时进行了对不同情况所采用的方法进行了综合与对比，由此为脑出血的临床诊治提供思考与借鉴。

According to the statistical analysis to cases of cerebral hemorrhage, and the research its pathology and physiology, we put forward an idea that operation is necessary to deal with cerebral hemorrhage, especially for hypertensive type. Moreover, the detailed dedivion of methods of surgical treatment, and their summerize and mutual comparision which are being references in use for its clinical treatment.

本文根据对脑出血病例的统计学研究，以及对其病理生理的探索，提出了对脑出血，尤其对高血压性脑出血进行外科手术治疗的必要性，并且详细阐述了针对其外科手术治疗的方法，同时进行了对不同情况所采用的方法进行了综合与对比，由此为脑出血的临床诊治提供思考与借鉴。

The glass and crystalline glass-solders of Li2O-ZnO-SiO2 system were prepared by conventional melt technology. The various factors effecting on glass ceramics and glass were investigated,which include glass chemical composition and heat-treatment and so on. And then by means of XRD, DTA, IR and SEM, the structure and properties of the glass and glass-ceramic were studied. The coefficient of linear expansion , transform temperature and soft temperature wer measured. And then the factors that effect the mechanical strength of glass-ceramic were discussed and the fluxion property was determined and the crystallization course and mechanism were discussed, which can provide certain therial reference value for practice producing and glass seals to glass, ceramics and metal.

用高温熔融法制备了Li_2O-ZnO-SiO_2系统玻璃及其结晶型玻璃焊料，探讨了各种因素(包括玻璃组成、热处理制度等)对微晶玻璃的结构和性能的影响；利用X射线衍射分析、DTA、红外光谱分析以及扫描电镜等现代测试手段，对玻璃的结构与性能进行研究，测定了玻璃及微晶玻璃的线膨胀系数、转变温度和软化温度，同时对影响微晶玻璃的强度的因素进行了探讨，并对该体系玻璃的流散性进行了测定，对它的析晶过程和析晶机理进行探讨，为实际生产和与玻璃、陶瓷及金属封接提供了一定的理论参考价值。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

Kangdai Ⅰ has protective function to the damaged neurons and astrocytes: Main results:(1) It has direct protective function to the damaged neurons. It can increase the activity and survival rate, decrease the mortality and the transudation rate of LDH in cultured medium and the strong positive cell count of NOS expression of injured neurons.(2) It has also directly protective function to the damaged astrocytes. It can increase the activity and survival rate and protein content in conditioned medium.(3) It can strengthen the ability of BDNF, GDNF, bFGF, HSP and IL-6expression in damaged astrocytes.(4) It can also strengthen obviously the expressions of NSE, bFGF-receptor and bc1-2, lower the expression of bax and caspase-3.(5) It can indirectly protect and restore the damaged neurons by astrocytes. Because the effect of ACMK (ACM interfered by Kangdai Ⅰ) is stronger than ACM+K (ACM associated with Kangdai Ⅰ).

抗呆Ⅰ号对受损的神经元和星形胶质细胞均具有保护作用：主要表现为：(1)对受损神经元具有直接的保护作用，可提高受损神经元的活性和存活率，降低细胞培养液LDH的漏出率、细胞死亡率和NOS染色强阳性细胞的表达量；(2)对受损的星形胶质细胞也有直接的保护作用，可提高其活性、存活率以及培养液蛋白质的含量；(3)能增强受损星形胶质细胞分泌BDNF、GDNF、bFGF、HSP和IL-6的能力；(4)可明显增强受损神经元对NSE、bFGF的受体和bc1-2的表达，降低受损神经元对bax和caspase-3的表达；(5)抗呆Ⅰ号可通过星形胶质细胞间接地保护和修复受损的神经元，因为在多数实验组中经抗呆Ⅰ号作用的ACM的作用远大于ACM与抗呆Ⅰ号联合应用的作用，统计学上具有显著性差异。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。