密度计的

For vortex-liquid phase,the E-J relation could be described according to the Kim-Anderson model:U=U0(1-J/JC).While for vortex-glass phase,the electric field decays verydramatically with decreasing current density,so it is difficult to be observed with theinstrument has limited precision.In the intermediate current region,the E-Jrelations of the both phases could be fitted in terms of a phenomenological model:U=(U0/μ)[μ-1],it was concluded that the sign of μ-value changes fromnegative to positive when the temperature decreases lower than the glass-transitiontemperature Tg,meanwhile,the activation energy U enhances dramatically.Whilein the large current region,Zeldov's logarithmic model was suitable for fitthing theE-J curves of the both phase.Moreover,there was no abrupt change of U or thecharacteristic current density IC.However,the effective radius of vortex line variesremarkably around Tg.

对于涡旋液体相，小电流区的E-J关系可以用计及反跳的Kim-Anderson模型U＝U0(1-J/JC)来描述，而对于涡旋玻璃相，小电流区的电场随电流密度减小而迅速衰减，以致很快脱离了实验测量精度的范围；在中等电流区，两相的E-J关系可以用共同的唯象过渡模型U＝(U0/μ)[μ-1]来拟合，得到在玻璃转变温度Tg两侧μ值由正到负的变化，同时激活能U在Tg以下迅速升高；在较高电流区，两相的E-J关系可以很好的用Zeldov对数模型来描述，拟合结果表明U在Tg两侧的趋势没有明显变化，但是涡旋线的有效半径发生了显著变化。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。