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Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ,限制性内切酶消化鉴定后,采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells , NSCs),G418 筛选阳性克隆,加入不同浓度的52氟胞嘧啶(52Flourocytosine , 52FC),MTT比色法测定NSCs的生存率。

Subependymal heterotopia can occur in isolation or with other neurologic and metabolic disorders.

室管膜下灰质异位可以孤立的发生或伴有其他的神经或代谢性疾病。

OBJECTIVE: To prepare the multi-lineage potential of neural stem cells for the use of intraventricular transplantation.

虽然新生动物脑内尤其是室管膜下区富含神经干细胞,也存在内源性神经干细胞的修复功能,但这种内源性的修复作用十分有限[1]。

Results The choroid plexus, hypophysis, area postrema and pineal body, namely, circumventricular organs and ependyma as well as pia mater layers were stained by the HRP reaction products.The HRP reaction products were observed in the tissue space of membrane mucosa nasi, around the fila olfactoria and venules, and within the venules under the light microscopy and electron microscopy, also. Scintillation count showed that the radioactivity of the CVOs was higher than that of the background. The radioautography showed that the scattered grains of silver existed within the cells of neurocyte layer also.

结果 在脑室周围器官及脑室周围室管膜、软脑膜层有HRP反应产物的聚集,在鼻中隔的鼻粘膜下组织间隙、嗅丝周围间隙及静脉周围和管腔内也可见到HRP反应产物;液闪计数显示,大白鼠的脉络丛、脑垂体、最后区、松果体等脑室周围器官放射性活度明显高于本底;放射自显影显示大白鼠脑组织神经层细胞内有散在分布的银颗粒。

Here we report that the secreted protein Slit repels neuronal precursors migrating from the anterior subventricular zone in the telencephalon to the olfactory bulb.

在此,我们讲述分泌蛋白裂将自前脑胞室管膜前下区移出的神经元前体细胞,排斥到嗅球。

As an immunomodulator NO may play a role like a double-edged sword since it expresses both in immuonchalleged and immunosspresed states. 2. After received external stimulus the morphology of ependyma of SFO could be changed and varied with time course, particularly its cellular apoptosis do occur and earlier than the ordinary areas of the brain. 3. Astrocytes in SFO could express cFos protein induced by LPS, suggesting the cells may maintain homeostasis in the brain in a longer period and modulate immune state of the brain. 4. As a CVO, SFO may sense changes of blood-bearing immunosignals earlier and instantly response to them, thus as one of windows of the brain SFO do take part in immunomodulation within the brain.

通过我们的实验结果我们可以得出这样一些初步的结论:1、一氧化氮作为免疫调质参与了机体免疫,它既能在免疫激发状态下又可在免疫抑制状态下进行表达提示,NO在脑内免疫调节中的作用可能是双向的。2、穹窿下器的室管膜形态在接受外界刺激后,随着时间的变化而不同,扫描电镜下的凋亡形态虽需进一步证实,但室管膜细胞确实发生了凋亡,并且穹窿下器发生的较早。3、穹窿下器内的星形胶质细胞在给予大肠杆菌后确实可以表达c-fos蛋白,并可能通过自身的激活来较长时间的维持体液的动态平衡,调节脑内免疫状态。4、穹窿下器作为感受性室周器官之一,能够较早的感受血携免疫信息的改变并及时作出反应,可能作为外周免疫信息进入脑内的窗口之一来参与脑的免疫调节。

Results 5 cases of ependymocytoma, 3 cases of astrocytoma, 1 medulloblastoma hemangioblastoma in 10 cases of intra-medullary tumor; 36 cases of neurogenic tumor, 11 cases of spinal meningioma in 47 cases of subdural extramedullary tumor; 2 cases of neurogenic tumor strided over scleromeninx;2 cases of extradural metastatic tumor, 1 cestodiasis, 2 cases of epidural hematoma, which were mistaken for hemangioma before surgical operation and 1 vascular malformation in 4 cases of intraspinal nontumorous occupying lesion.

结果:10例髓内肿瘤中,室管膜瘤5例;星形细胞瘤3例;血管母细胞瘤1例;成神经管细胞瘤1例。47例脊髓外硬膜下肿瘤中,神经源性肿瘤36例;脊膜瘤11例。2例跨硬膜内外生长的神经源性肿瘤2例。硬膜外的转移瘤2例。4例椎管内非肿瘤性占位性病变中,椎管内绦虫病1例;硬膜外血肿2例,术前误为血管瘤;血管畸形1例。

Results: The number of BrdU-positive cells in the subventricular zone and in the subgranular zone of dentate gyrus,and the numbers of ChAT-positive cells in medial septumand diagonal band of the basal forebrain were significantly decreased in lead exposed group P

结果:铅中毒大鼠齿状回的颗粒下层、侧脑室室管膜下区的BrdU阳性细胞数、基底前脑内侧隔核和斜角带核的ChAT阳性细胞数以及大脑皮质和海马齿状回NF阳性神经元数、嗅球BrdU+NF阳性细胞数目以及学习记忆能力明显下降,GFAP阳性细胞反常升高P

Lateral tumors in adults often appear extraaxial and may simulate meningiomas of the cerebellopontine angle or tentorium, acoustic neuromas, ependymomas, glomus jugulare tumors or metastases, solid hemangioblastoma and choroid plexus papilloma.

位于外侧的肿瘤经常表现类似于轴位病变,与桥小脑角区或幕下脑膜瘤、听神经瘤、室管膜瘤、颈静脉球瘤或转移瘤、实性血管母、以及脉络膜乳头状瘤相类似。

These implied that CCK synthesis in somas of the PVN and the releasing in terminals of the ME during scalding stress were increased.The emergence of CCK-containing CSF-CNs might provide their secretory products a new pathway to cerebrospinal fluid after scald and the CCK,through the CSF pathway,might involve scald stress by modulating the metabolism and activity of the adjacent peptidergic system controlling the pituitary.

为此,本实验用免疫细胞化学和显微图像分析技术研究烫伤大鼠PVN和第三脑室室管膜内CSF-CNs中CCK含量的变化,旨在探讨PVN中CCK是否参与烫伤应激反应,并为CSF-CNs在应激状态下如何平衡脑-脑脊液神经体液回路提供形态学依据。

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