室温

The content ofone liver protein band (R_f=0.79) at 1℃was significantly higher than at room temperature (P＜0.05).On the 2-D native-PAGE/SDS-PAGE gel, the content of P spot (MW=51kDa, R_1=0.47on the 1D native PAGE gel) at 1℃was significantly higher than at room temperature (P＜0.05), and the content of N spot (MW=65 kDa, R_f=0,94 on the 1D native PAGE gel) at roomtemperature was significantly higher than at 1℃. These protein spots may be related with the adaptation to the low temperature of the Heilongjiang wood frog.

黑龙江林蛙肝脏蛋白电泳显示，胶浓度6％的肝脏PAGE图谱分辨率高、谱带多，可分辨出15～19条谱带，其中1℃组R_f=0.79蛋白含量比室温组显著增加(P＜0.05)。2-D native-PAGE／SDS-PAGE电泳时，胶浓度为12％时分离效果较好，蛋白斑点较多。1℃组中P蛋白斑点(MW=51kDa，一向PAGE的R_f=0.47)显著性上调(P＜0.05)，N蛋白斑点(MW=65kDa，一向PAGE的R_f=0.94)显著性下调(P＜0.05)，推测这两种蛋白可能与黑龙江林蛙在低温环境下的适应性变化有关。

It is significance to further study the tolerance mechanism ofHeilongjiang wood frog during hibernation. Its cold-tolerant mechanism has an importantapplied value and a great potential for exploitation and utilization.We choosed Heilongjiang wood frogs from Yichun, Heilongjiang province.

本试验选取黑龙江省伊春地区黑龙江林蛙为研究材料，观察黑龙江林蛙在环境温度为室温(14℃)、1℃和-2℃条件下的存活率，并采用2-D native-PAGE／SDS-PAGE电泳、酶活性比色法、同工酶电泳等生化技术检测不同温度环境下肝脏蛋白质及代谢酶的活性变化。

However, its room temperature brittleness and poor workability limit its industrial application.

但高硅钢的室温脆性和低的热加工性能严重影响了其在工业领域的应用。

At room temperature,effects of soil burying storage treatment on Chinese yam were studied.

在室温条件下，研究了山药的埋藏保鲜方法。

Effects of yttrium on microstructure, mechanical properties at room temperature and elevated temperature, cast properties and corrosion resistance of Mg-Al system alloys are reviewed in the paper.

综述了钇对Mg-A1系合金的组织、室温和高温力学性能、铸造性能以及耐腐蚀性能的影响。

Highly oriented ZnO nanoneedle/nanorods arrays have been fabricated by direct oxidation of zinc foil in alkaline zincate ion solution at near room temperature.

在室温附近溶液中直接界面反应大面积的制备了ZnO纳米针/棒阵列。

Zirconium oxide films were deposited on SiO2 substrates using high vacuum electron beam evaporation at room temperature.

在室温下采用电子束蒸发的办法制备二氧化锆(ZrO2)薄膜。

Photoluminescence measurements show that two strong PL bands were emitted at room temperature in the ordered porous zirconium oxide material.

利用荧光分光光度仪，我们首次在室温下观测到了有序多孔氧化锆材料的荧光发射谱。

INTERVENTIONS: In experimental group, the emetic, cisplatin, was injected abdominally 10 mg/kg. In the control, the physiological saline of same dose was injected. Afterwards, the activity changes in rats were observed at room temperature, quiet and light-avoided environment. Six hours later, the brain tissue was collected for frozen continuous sectioning. Immunohistochemical staining method was used to observe the distribution of Fos positive neurons in brainstem and forebrain nuclei and to count positive cell.

干预：实验组腹腔注射催吐剂顺氯氨铂10 mg/kg，对照组注射等剂量的生理盐水后，于室温、安静、避光环境下观察大鼠的行为学变化。6 h后取大鼠脑组织，进行冷冻连续切片，用免疫组织化学染色方法观察大鼠脑干和前脑核团的Fos阳性神经元的分布，并进行阳性细胞记数。

Water absorbency in distilled water and tap water in 30 min was about 1000 g/g and 200 g/g, respectively.

制得的高吸水性树脂在室温下30min每克吸蒸馏水和自来水分别约为其自身质量的1000倍和200g倍。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。