实验法

Basic topics and ideas in the design of experiments: randomization and randomization tests; the validity and analysis of randomized experiments; randomized blocks; Latin and Greco-Latin squares; plot techniques; factorial experiments; the use of confounding and response surface methodology; weighing

实验设计：试验设计的基本思想和论题：随机化和随机化检验；随机试验的效度与分析；随机区组设计；拉丁方和希腊拉丁方；制图技术；析因试验，混杂法和反应曲面法的运用；加权设计，方格设计和不完全区组设计，部分平衡的不完全区组设计。

Methods The qualitative analyses of Kudzuvine root,Large-flowered skullcap root and Chinese goldthread of "Ge Gen Qin Lian" pocket preparation were finished.The reseachers analysed them with TLC method. Then they determined puerarin content of Kudzuvine root by TLC scan.

本实验采用薄层层析法对葛根芩连袋泡剂的主要中药葛根、黄芩及黄连进行了定性分析，并通过荧光淬灭薄层扫描法测定了君药——葛根中葛根素的含量。

In this paper a method is outlined to compute wall-interference in closed low speed wind tunnel for 3D high lift test using wall pressure at optimum points and the influence func-tion.

研究了根据最佳点壁压和影响函数对三维模型低速高升力测力实验进行洞壁干扰修正的方法（简称壁压影响函数法，WPIF法）。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用，按美国ASTM标准制备NiTi、317L合金的金属浸提液；&组织块培养法&原代培养食管壁疤痕的成纤维细胞，传代后以金属浸提液进行培养，分组后分别培养4、24、48、72 h，MTT法检测不同培养时间后Fb增殖功能的变化；按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验，即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间，术后2、12周取出包埋组织，分析试件周围组织的病理变化，并进行胶原纤维染色，观察纤维形成状况。

The proliferation of cells were detected by trypan blue exclusion, methyl thiazolyl tetrazolium assay, Hoechst33342 fluorescence staining and flow cytometry method.

利用台盼兰排斥试验、噻唑蓝比色法、Hoechst33342 荧光染色、流式细胞术测细胞周期法等实验技术分别检测了不同浓度AA 和DHA 对大鼠前体脂肪细胞活力、细胞周期及凋亡的影响。

At the endpoint, the thymus, spleen and uterus were weighed separately; the femoral dry weight, bone-calcium, phosphor, magnesium and hydroxyproline contents of femoral were measured; the histomorphological features of bone were observed and the biochemical indexes in serum (Ca, P, ALP, TRAP, E2, BGP) were determined after the mice were given SAR at the dose of 10, 30, 90 mgkg^(-1) respectively, and were compared with the model group, the normal control and the positive control. RESULTS: The osteoporosis of high turnover type was formed with feeding retinoic acid to experimental mice. Compared with the model group, the lessening of femoral dry weight, the short femoral transverse diameter, the decrease of bone mineral and bone-hydroxyproline of SAR groups were improved.

维A酸105mgkg^(-1致小鼠骨质疏松模型，SAR10、30、90mgkg^(-1灌胃给药，实验过程监测体重，2周后以比色法测定血清钙、磷含量和碱性磷酸酶、抗酒石酸酸性磷酸酶活性，竞争放射免疫法测定血清中骨钙素、雌二醇(s-E2)含量，处死动物后取脾、胸腺、子宫称重，取股骨进行骨组织形态学观察，测定并比较骨计量学和骨钙、骨磷、骨镁及骨弃脯氨酸含量。

Chapter Three. The Studies on Gene Therapy for Retinal Degeneration of the Mice and It's Mechanism Objective: To evaluate the abilities of adeno-associated virtus carrying the basic fibroblast growth factor gene as a therapy for retinal photoreceptors in slow retinal degerneration mice on the basis of the experiment results as mentioned above. Methods: On the born day of rds mice, IPE cells were isolated with enzyme-assisted microdissection from pigs and were cultured.

第三章 视网膜色素变性小鼠的基因治疗及其机制目的：在体外实验证明AAV载体可以高效安全转染IPE细胞并且可以表达具有生物活性的bFGF的基础上，应用AAV载体携带bFGF基因以先体外，后体内法和体内法两种方式进行视网膜色素变性鼠(retinal degemeration slow，rds)的基因治疗，在分子生物学、形态学和功能学上进行基因治疗的初步观察方法：在rds鼠出生的当天取眼球，采用酶辅助的显微分离方法分离培养IPE细胞。

The proliferation detected using MTT method, the DNA synthesis was evaluated by -thymidine incorporation in cultured cells.

实验用不同组大鼠主动脉制备离体培养平滑肌细胞，用MTT法分析细胞增殖，用-TdR掺入法分析细胞DNA合成。

From factorial experiments, it was observed that in conventional extrusion treatment, defatted soy flour, aqueous acidic leached soy protein concentrate and soy protein isolate was able to be texturized, whereas SPC was not.

通过挤压实验可知，在常规的挤压条件下，低温粕、酸法SPC和大豆分离蛋白均可实现组织化，而醇法SPC无法实现有效的组织化。

A novel method of measuring plus lens's focus is given, which is based on LCD, CCD, and digital image processing It is also a discussion on photoelectric method of automatically measuring parameters of an optic system In this method, a LCD is used as a reticle of the collimator, while the image on the accepting side is transformed into digital image and is sent into a computer The focus can finally be calculated out through digital image processing and analysis The method of measurement is introduce...

讨论了一种基于LCD、CCD和数字图像处理的正透镜焦距测量方法，是光电法自动化测量光学系统多个参数的一种有益的探讨。用液晶屏显示分划图形取代平行光管的分划板，用CCD在接收方采集数字图像并送入计算机，通过图像处理和分析得出被测透镜的焦距。运用叠加的方法提高图像的信噪比；结合重心法和平行线拟合方法求取平行线对的间距；应用高精度焦距标准透镜标定数字图像和实际像之间的比例关系。详细介绍了测量和图像处理的方法，进行了公式推导和误差分析，并给出了实验结果

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。