实验法

In the one half part, according to the strontium nature, thermodynamic calculation of correlative reaction and the principle of the vacuum aluminothermy reduction process, with the laboratory findings of preparation strontium by vacuum aluminothermy reduction compared and analyzed in different parameter conditions, a variety of factors which effect the decomposition rate of SrCO_3 and the percent reduction of strontia are gotten out. The factors include that the decomposition rate of SrCO_3 is influenced by different addition and pressure. They also include that the percent reduction of strontia is influenced by excess coefficient of reducing agent aluminium powder, the particle size of raw material or reducing agent, the pressure of barbecuing, temperature and reduction time. According to those, we can draw the conclusion as follows:(1) Decomposition temperature of SrCO_3 is decreased by adding carbon and alumina in different degree.(2) With quantitative carbon added under the vacuum condition, the decomposition temperature of SrCO_3 is decreased obviously, which can fall 150℃ compared with atmospheric pressure.(3) In keeping 1150℃ of 2.5h, under the 5Pa vacuum condition, the decomposition rate of SrCO_3 nearly keeps in 99% steadily.

在前半部分的真空铝热还原法中，根据锶的性质、相关反应的热力学计算及真空铝热还原法原理，通过对不同参数条件下的真空铝热还原法生产金属锶的实验结果的分析比较，得出了影响SrCO_3的分解率和氧化锶的还原率的种种因素，具体因素包括不同添加剂、不同气压对SrCO_3分解率的影响，还原剂铝粉的过量系数、原料与还原剂的粒度、制团压力、温度和还原时间等对氧化锶还原率的影响，得出具体结论如下：(1)添加碳和Al_2O_3能不同程度的降低SrCO_3的分解温度；(2)真空条件下加入一定量的碳可显著降低SrCO_3的分解温度，比之常压下可降低150℃之多；(3)在1150℃恒温2.5h、真空度达到5Pa的条件下，SrCO_3的分解率几乎保持稳定在99％；(4)氧化锶的还原率随还原剂过量系数的增大而增大，但是趋势越来越小，当过量系数超过25％后，氧化锶的还原率几乎不再增加；(5)氧化锶的还原率随原料与还原剂的粒度的变细而增加；(6)氧化锶的还原率随制团压力的增加而增加，但超过一定值后，氧化锶的还原率反而会下降；(7)氧化锶的还原率随还原温度的升高而增加；(8)氧化锶的还原率随还原时间延长而增加，在本实验条件下，超过2.5h趋于稳定。

Simulation experiments make sure that the dilatometer is able to detect the expansion changes in low temperature. The high temperature reheating process with dilatometer analysis indicate that the firing temperature are little higher than 900℃ for one sample and lower than 900℃ for other five samples with firing temperature ranges of 550~650℃, 650~750℃ and 750~900℃. The results from mineral composition analysis of the samples by X-ray diffraction provide further evidence of the reliability of the thermal expansion measurement.

模拟实验表明，所采用的热膨胀仪能适应低温法测温实验；用热膨胀高温法测出早期陶片中有一块样品原始烧成温度略高于900℃，其余5块样品皆低于900℃；通过热膨胀低温法较为准确地测出这五块样品原始烧成温度是分别介于550~650℃、650~750℃、750~900℃三个范围，而X射线衍射分析结果从物相成分的角度证明了测温结果的可靠性。

An efficient protective material for metal relics was studied in the paper. Volatile rust proofing sieve, vapor inhabitability of mass loss and closed space volatile weight-loss methods were adopted to study many monocomponent volatiles corrosion inhibitors . The best VCI was chosen from them. By mixing, the two composite VCIs were made for iron and copper.

本研究通过采用气相甄别法、失重法和封闭空间挥发减量法实验筛选单组分气相缓蚀剂并进行复配实验，分别制备出铁质文物和铜器文物用的复合气相缓蚀剂，其中铁质文物复合气相缓蚀剂Ⅰ由有机胺A和乌洛脱品以质量比1：1组成，其缓蚀率为96.5％，铜器文物复合气相缓蚀剂Ⅱ由BTA和酚类W以质量比1：1制成，其缓蚀率为94.5％。

The research carried out an experiment of extracting water from inland limnetic wetlands using TM remote sensing imagery based on many arithmetic,and concluded that spectrum classification was the best among the area accuracy index,extraction accuracy index and visual effect index,the following was single band threshold and vegetation index method,however,multi-band relationship and water index method was the worst.

研究基于TM遥感影像，运用多种方法针对典型内陆淡水湿地的水体信息进行了提取实验，通过对实验结果的分析得出：在面积的准确性、提取的准确度以及视觉效果3种指标下，光谱分类法较其它方法效果要好，其次为单波段阈值分析法与植被指数法，较差的是多波段谱间关系法与水体指数法；影响提取效果的主要原因是湿地水体提取不够完全，这是由影像的分辨率及湿地特殊的水文条件所造成的，采用像元分解及多源遥感数据融合技术将成为提高水体提取精度的重要手段。

A549 cell line was stimulated with LPS (10 μg/mL) and then treated with Rs504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor-1, monocyte chemoattractant protein-2, and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry.

体外实验选用A549细胞系，应用LPS(10μg/mL)刺激合并RS504393(10μg/mL)治疗6 h后，流式细胞仪技术检测CCR1和CCR2的表达，ELISA法检测细胞培养上清液内白介素-8的浓度；体内实验选用C57BL/6J小鼠，腹腔注射RS504393(5 mg/kg)预处理30 min后经鼻滴入JPS(5 mg/kg)，LPS刺激4 h后收集BALF和肺脏标本，计数BALF内细胞总数，应用BCA法检测BALF内蛋白浓度，Realtime-RT PCR和ELISA法检测BALF和肺脏IL-1β、凝血酶原激活物抑制物-1和单核细胞趋化蛋白-2的表达。

Parallel to the nomographic method, the numeri- cal calculating method is also put forward here. As an example, the lead and aluminium rings are pressed with oil lubrication or with- out. The results of data processing by nomogram and the numerical calculating are compared.

在线图法处理实验数据的基础上，提出了理论计算法，并对铅、铝两种材料在有无润滑剂条件下进行了圆环压缩试验，比较了用线图法和理论计算法对实验数据的处理结果，指出了各种方法的优缺点。

A series of indexes reflecting free radical changes, anti-oxidations, Immune function, endocrine function was studied by Chemiluminescence method, immunoturbidimetry, ANAE dye method, micro-hemagglutination inhibition test and radio immunoassay in the chicken with encephalomalicia induced by giving the feed being deficient in VE and extra fish oil artificially. The pathological changes including microscopic and ultramicroscopic changes in the relative organs and tissues were observed.

本实验以一日龄150只海兰兰蛋鸡为实验动物，用低维生素E且添加鱼油日粮饲喂雏鸡，复制脑软化症动物模型，采用化学发光法、免疫浊度测定法、酸性α-醋酸萘脂酶染色法、微量血凝抑制试验、放射免疫测定技术等方法系统地检测了脑软化雏鸡的自由基代谢和抗氧化功能、红细胞免疫功能、细胞免疫功能、体液免疫功能及非特异性免疫功能等指标，并对患雏病理组织进行了显微及超微结构观察。

Methods: We collected 121 of clinical isolates of Enterobacter, including 87 isolates Enterobacter cloacae, 34 isolates Enterobacter aerogenes, all of the isolates were detected by cefoxitin three-dimension test, drug resistance phenotypes estimation, cloxacillin disc synergy test and cloxacillin-potentiated disc diffusion test respectively to find high-level AmpC β-lactamase isolates, drug resistance phenotypes estimation were also used to detected high induction producing AmpC β-lactamases isolates, meanwhile, we took three-dimension test as standard method, and compared to other methods, then calculated the coincidence rates, sensitivities and specificities of them.

采用头孢西丁三维实验、纸片耐药表型推断法、邻氯西林纸片协同法、双纸片邻氯西林增效试验分别检测高产AmpCβ-内酰胺酶株，获得高产AmpC酶在肠杆菌属细菌中的表达状况。采用纸片耐药表型推断法检测诱导和高产AmpC酶，同时以头孢西丁三维实验为标准，其他三种方法与之相比较，分析它们检测高产AmpC酶的符合率、灵敏度和特异性。

The method of reduced ammonia marinate was selected to do the distill experiment of cobalt and of other metallic elements from ocean manganese nodules and the iron in marinated solution was removed with ammoniojarosite and cobalt was separated from other metallic element with method of sulfuration deposition.

选择还原氨浸法对大洋锰结核中的多金属进行了浸取实验，并用铵黄铁矾法对浸取液进行了除铁及用硫化沉淀法对钴进行分离提取等实验。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。