实验法

The main object of this research is to investigate synthesis of Cu23Cl Cu and CuO nanoparticles through electrospinning and heat-treated method The research is mainly focused on preparative parameter and the change of heat-treated temperature to form the nanoparticles inlaid in silicon fibers the shape and color change are dicussed First of all to prepare the Polyvinyl butyral and silica dioxide complex nanofiber by electrospinning and then to investigate different heat-treated temperature and reactive time to being an influence on the products The results detected by SEM XRD TEM EDS FTIR combined sol-gel process and electrospinning can prepare Polyvinyl butyral and silica complex nanofiber The experimental result is found when heat-treated temperature is 100~4750C it can produce Cu23Cl nanoparticles; Above 4750C~ 4900C it can produce Cu nanoparticles; Above 450~7000C it can produce CuO nanoparticles And the viscosity is lain between 20~40cp and the sol-gel process time is 3hr it can produce the thinner fibers The average diameter of the fibers are 107 88±21 01nm;Due to the nanoparticles inlaid in the silica fibers the thinner fibers can be inlaid the smaller nanoparticles so this is the result that the experiment is expected To calcine the complex fibers is to produce surface silica fibers contain Cu23Cl Cu and CuO nanoparticles due to surface plasma resonance it make the color of the fibers become yellowish green from light white green turn into the red nuclear finally As the experimental result to utilize sol-gel process combine electrospinning can produce porous silica fibers contain Cu23Cl Cu and CuO nanoparticles

本研究旨在探讨「利用放电纺丝和热处理法来合成Cu23Cl、Cu和CuO奈米粒」之研究，实验著重在制备参数与热处理温度变化对所形成的奈米粒镶嵌在二氧化矽纤维中的形态与颜色变化探讨。首先，利用放电纺丝法制备出聚乙烯醇缩丁醛及二氧化矽之奈米纤维，比较不同的热处理温度与反应时间的改变对产物生成产生影响，进而研究不同热处理温度和时间对生成奈米粒的影响。产物经由SEM、XRD、TEM、EDS和FTIR等仪器分析结果显示，结合溶胶-凝胶法(sol-gel process)和放电纺丝法可产生聚乙烯醇缩丁醛及二氧化矽复合奈米纤维。实验结果发现，若热处理温度在100~4750C下可得到Cu23Cl奈米粒，475~4900C 可得Cu奈米粒，450~7000C以上可得 CuO奈米粒。而黏度介於20~40cp间和溶胶-凝胶时间为3hr时，可产生直径比较细的纤维，纤维直径为107 88±21 01nm；且由於奈米金属颗粒镶嵌在二氧化矽纤维中，直径比较细的纤维，可以得到比较小的奈米金属颗粒，这与实验预期相符。而锻烧此复合物产生多孔的二氧化矽纤维并包含Cu23Cl、Cu和CuO奈米微粒时，由於表面电浆子共震关系，而使纤维颜色由淡白黄绿色变成黄绿色，再变红褐色。由实验结果得知，利用溶胶-凝胶法(sol-gel process)结合放电纺丝法和不同热处理温度，可产生Cu23Cl、Cu和CuO奈米粒镶嵌在二氧化矽纤维。

It solves the problem that the unitary contour presentation can not correctly extract face contour in a face image which suffers from scale, rotation etc. The definition of the internal and external energy function is provided. At the same time, the global matching algorithm and local matching algorithm is given. The experiment shows that this presentation and the accompanying matching algorithm can be used to extract the face contour very well. So the image segmentation can be implemented by using it.②By analyzing the recognition principle of PCA method, we can conclude that the face images coming from different surrounding consist of different face image space. This is the essential reason that makes the generality of PCA method worse. Also, we give a measurement means to measure the distance from different face image space, so we can analyze face image space more conveniently.③We also construct various scale models and rotation pose models to detect the scale and rotating angle of face image to be recognized. The experiment results show that the detecting precision is very high. So it is good for face image feature extraction and face image representation.④Similarly, we construct local feature models of face image and utilize them to detect the local feature of face image. At the same time, we put forward a novel face image local feature detection algorithm, locating step by step. The experiment results show that this method can accurately detect the location of local face feature in a image.⑤A novel face image presentation model, dual attribute graph , is put forward. Firstly, it utilizes attribute graph to present the face image, then exact the local principal component coefficient and Gabor transform coefficient of thc pixels which corresponds to the nodes of the graph as the attribute of the nodes. This representation fully makes use of the statistical characteristic of the local face feature and utilizes Gabor transform to present the topographical structure of face image. So DAG has more general property.⑥Based on the DAG presentation, we give a DAG matching function and matching algorithm. During the design of the function and algorithm, the noise factor, e. g., lighting, scale and rotation pose are considered and tried to be eliminated. So the algorithm can give more general property.⑦A general face image recognition system is implemented. The experiment show the system can get better recognition performance under the noise surrounding of lighting, scale and rotation pose.

本文在上述研究的基础上，取得了如下主要研究成果：①构造了一个通用的人脸轮廓模型表示，解决了由于人脸图象尺度、旋转等因素而使得仅用单一轮廓表示无法正确提取人脸轮廓的问题，并给出了模型内、外能函数的定义，同时给出了模型的全局与局部匹配算法，实验表明，使用这种表示形式以及匹配算法，能够较好地提取人脸图象的轮廓，可实际用于人脸图象的分割；②深入分析了PCA方法的识别机制，得出不同成象条件下的人脸图象构成不同的人脸图象空间的结论，同时指出这也是造成PCA方法通用性较差的本质原因，并给出了不同人脸空间距离的一种度量方法，使用该度量方法能够直观地对人脸图象空间进行分析；③构造了各种尺度模板、旋转姿势模板以用于探测待识人脸图象的尺度、旋转角度，实验结果表明，探测精确度很高，从而有利于人脸图象特征提取，以及图象的有效表示；④构造了人脸图象的各局部特征模板，用于人脸图象局部特征的探测；同时提出了一种新的人脸图象局部特征探测法---逐步求精定位法，实验结果表明，使用这种方法能够精确地得到人脸图象各局部特征的位置；⑤提出了一种新的人脸图象表示法---双属性图表示法；利用属性图来表示人脸图象，并提取图节点对应图象位置的局部主成分特征系数以及Gabor变换系数作为图节点的属性，这种表示方法充分利用了人脸图象的局部特征的统计特性，并且使用Gabor变换来反映人脸图象的拓扑结构，从而使得双属性图表示法具有较强的通用性；⑥在双属性图表示的基础上，给出双属性图匹配函数及匹配算法，在函数及算法设计过程中，考虑并解决了光照、尺度、旋转姿势变化等因素对人脸图象识别的影响，使得匹配算法具有较强的通用性；⑦设计并实现了一个通用的人脸图象识别系统，实验结果表明，该系统在图象光照、尺度、旋转姿势情况下，得到了较好的识别效果。

A series of theoretical and experimental studies on the microstrip patch antennas with dielectric superstrates is carried out systematically. Firstly, a comprehensive survey of the microstrip antenna technology and its theories are presented. Then, two analysis techniques, named as the spectral domain Green's function method and the spectral domain integral equation method are developed. The SDGF method is a simplified analysis technique, which takes advantage of the spectral domain immittance approach to find the dyadic Green's function for the stratified media, and obtains the complete closed-form formulas for radiation patterns, directivity, efficiency, gain etc. Some interesting calculated results are presented. The SDIE method is capable of determining resonant frequency, radiation characteristics, input impedance etc, for the covered microstrip patch antennas. Comparing with other full-wave analyses it reduced computation and mathematical labour remarkably. The singularities near the pole location of the surface-wave modes and the difficulty of the integral computation over infinite range of oscillating integrands are dealt with actively and documented well. Results predicted by the SDIE method agree extremely well with our experimental results for resonant frequencies of various superstrates. The superstrate effects on impedance and radiation characteristics also have been investigated experimentally so that the gap due to lack of measured data and computed results for multi-layered superstrate has been filled to some degrees and two useful observations have been achieved for weakening the resonant frequency shift and optimizing the microstrip antenna gain. As a meaningful application, the design and analysis of a 4×4 patch array are introduced.

实用微带天线普遍加介质覆盖层来防护热，物理损伤和环境影响，也可能在飞行或严酷气候条件下自然形成覆盖层本论文对带介质覆盖层的微带贴片天线系统地作了一系列理论和实验研究首先，对微带天线技术和理论作了全面的综述然后，发展了两种分析方法，称为谱域格林函数法和谱域积分方程法SDGF法是一种简化的分析技术，它利用谱域导抗法来求得分层媒质的并矢格林函数，并得出方向图、方向性系数、增益等参数的完整的闭式公式，给出了一些令人感兴趣的计算结果SDIE法能确定介质覆盖微带天线的谐振频率、辐射特性和输入阻抗等与其它全波分析方法相比，它明显减小了计算量和数学工作量已有效地处理了表面波模极点附近的奇异性和对振荡的被积函数的无限区间积分计算的困难对于不同覆盖层情况下的谐振频率，由SDIE法所预示的结果与我们的实验结果吻合得很好对于覆盖层对阻抗和辐射特性的影响也已作了实验研究这在某种程度上填补了缺少实验数据和计算结果之间的间隙，并得出了缓解谐振频率偏移和优化天线增益的两点有用的结论作为有意义的应用，介绍了一个4×4元矩形贴片天线阵的设计和分析。

In this paper, first of all, the common approaches of demonstrating experiment in general physics are systematically analyzed, including magnification, comparison, transformation, simulation, balance and unbalance, stroboscope, trace-show, projection, etc. In addition, a great number of practical examples exhibit that these approaches can be successfully applied to the physical demonstrating experiments. Then, some specific experimental means are thoroughly investigated from the view of technique, combined with the content of demonstrating experiments in general physics.

本文首先对普通物理演示实验中常用的实验方法如放大法、比较法、转换法、模拟法、平衡法和失衡法、频闪法、示踪法、投影法等做了比较详细、完整的分析，并通过大量实例说明了这些方法在物理演示实验中的应用；然后结合普通物理演示实验内容就一些具体的实验方法从技术实现的角度进行了分析研究。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

It indicates the distinct element method simulation model of charging of bell-less top blast furnace may replace the model experiment that takes very long time, and spends a great amount of both manpower and material resources to research the law of charging of bell-less top. The distinct element method simulation is made for opening furnace charging of reality bell-less top blast furnace. The result of simulation is also very identical with that of measuring.

本文在高炉无钟炉顶实验模型上进行了单环和多环布料实验研究，同时运用离散单元法模拟模型对实验进行了数值模拟，得到的炉料分布和料面形状的实验结果与模拟结果相当吻合，这证明了离散单元法在高炉无钟炉顶布料中的应用是可行的，并且表明运用高炉无钟炉顶布料离散单元法模拟模型可以替代费时、费钱、费力的物理模型实验，进行无钟炉顶布料规律研究。

It is one of the most important method in chemical research. The course matches Instrument Analysis course. We set up 16 experiments: atomic absorption spectrum, atomic emission spectrum, atomic fluorescent, ultraviolet-visible absorption spectrum, infra-red absorption spectrum, and molecular fluorescent which are for photometric analysis; vottammetric analysis , coulometry analysis ,volt-ampere analysis and polarogarphy for electroanalytical chemistry; Gas chromatography and liquid chromatography for chromatographic analysis.

本实验内容与仪器分析理论课内容相配套，针对光化学分析开设了原子吸收光谱、原子发射光谱、原子荧光、紫外-可见吸收光谱、红外吸收光谱、分子荧光光谱等实验；针对电化学分析开设了电位分析法、库仑分析法、伏安和极谱分析法等实验；针对色谱分析开设了气相色谱和液相色谱分析等实验，共开设了16个实验。

The effects of a nonionic surfactant on the seeded precipitation rate of sodium aluminate solution, the particle size and morphology of the products were studied.

采用对比实验法研究非离子型表面活性剂对铝酸钠溶液晶种分解率及其产品粒度和形貌的影响。

However, no further effort has been made to ascertain whether service recovery will have different effects upon complainants and non-complainants.

本文通过情景模拟实验法，对投诉者与不投诉者对服务补救的评价及服务补救对两类顾客所产生的效果进行比较分析，结果发现对于同样的服务补救措施，投诉者对于补救水平的评价并不低于不投诉者；且相对于不投诉者而言，投诉者的满意度和再购买意愿也并非更难恢复。

Based on flow-through method under the laboratory conditions, the energy budgets were measured and the bioenergetics model s were described for seven species of fish distributed in the Bohai Sea and Yellow Sea ecosystem.

根据室内流水实验法，定量研究了黄、渤海生态系统7种鱼类能量收支组分，并建立了相应的生物能量学模式。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。