实验室方法

To study the ecophysiological effects of low temperature stress on podocarpus fleuryi Hickel seedlings, two-year-old seedlings were selected as experimental material. We conducted comparative study. On the one hand, seedlings were separately placed in the plots located at the top, half-way-up and the foot of Jinyun Mountain (different altitudes at Jinyun Mountain led to the difference in air temperature) and subjected to winter low temperature stress. On the other hand, in laboratory, seedlings were exposed to simulated low temperature stress which were artificially controlled .

为了探讨低温胁迫对长叶竹柏幼苗的生理生态影响，本试验选取长叶竹柏的二龄幼苗为试验材料，在试验方法上采取了对比研究方法：一方面，利用缙云山不同的海拔造成的温度差异，分别将幼苗置于山顶、山腰和山脚下三个样地中，进行野外冬季自然低温胁迫处理；另一方面，在实验室中，利用人工控制的不同低温进行模拟低温胁迫处理。

The method of gas chromatography has been set up under laboratory condition to determine the content of oligosaccharides (sucrose,raffinose and stachyose)in soybean products.

本研究在实验室条件下建立了一个测定大豆及其产品中大豆寡糖气相色谱分析方法，以此对三种大豆寡糖进行了分离和测定，并制作了标准曲线，同时还对方法的精确度、回收率和稳定性进行了分析测定。

By systemic researching and simulating experiment, the text gains such results as follows,1. On the base of sufficiency research on the oil-gas hydrocarbon systemic chemistry composing and the physical chemistry characters, we traverse the mechanism of the paraffin's deposition. Then we consider that paraffin deposition is a complex process ofphysical chemistry, which includes more than one single mechanism.2. Traversing and comparing the literature in detail on different factors that has effect on paraffin deposition, the available experimental methods on paraffin deposition, prevention of paraffin deposition, and removing of the deposited paraffin on the spot.3. Ameliorated the laser equipment of solid particle inspecting system and the corresponding test methods. Determine WCT of dead oil and live oil from KS101 well under different pressure with this system. The result implies that dead oil has opposite regulation on paraffin deposition temperature to live oil.4. A vapor-liquid-solid envelop is drawn by superposition regular vapor-liquid envelop and WCT curve thereby explained that the departure and quick raising of the curve in the low temperature zone is caused by the appearance of paraffin in solid phase.5. Estimation is made to determine the depth of paraffin deposition in the well.

通过系统的研究和实验模拟，本文取得了以下研究成果：(1)在充分调研油气烃类体系的化学组成及其物理化学特征的基础上，详细研究了石蜡沉降的机理，认为石蜡沉积是一个复杂的物理化学过程，是多种机理共同作用的结果；(2)深入调研并综合阐述了影响石蜡固相沉积的各种主要因素，对目前国内外常用的有机固相沉积实验研究方法和现场清防蜡措施也做出详尽的描述，并加以对比评价；(3)改进了油气藏开发重点实验室自行研制组装的高温高压下测定固相沉积的激光装置和测试方法，并用该套设备测定出柯深101井的井口脱气油样和凝析气平衡油样不同压力下的析蜡点，实验结果表明，脱气油和平衡油析蜡规律相反；(4)将常规气-液两相相图和析蜡曲线叠加得到了气—液—固三相相图，从而解释了气-液两相等液量线在低温区域偏离正常并快速上翘是由于体系有石蜡固相出现；(5)利用实验测试的析蜡温度预测析蜡位置，表明只有在距井口465m油管以上会有蜡析出。

Methods Multi-level air microorganism sampler was used to carry out laboratory and on-the-spot tests.

方法用多级空气微生物采样器方法进行了实验室和现场试验检测。

The method is in simular experimented in the water circulating system consisting of the water pump and the stainless steel pipeline in the laboratory. The result proves the information fusion technology is remarkably superior to the method of single leak detection.

在实验室用水泵和不锈钢管道组成的水循环系统进行了模拟实验，通过对比验证了信息融合检漏方法比单一检漏方法具有明显的优越性。

Fan YS,Davis LM,Shows TB.Mapping small DNA sequences by fluorescence in situ hybridization directly on banded metaphase chromosome.Proc Nat1 Acad Sci USA,1990,87∶6223-6227.5 Cherif D,Julier C,Delattre O,et al.Simultaneous localization of cosmids and chromosome R-banding by fluorescence microscopy:Application to regional mapping of human chromosome 11. Proc Nat1 Acad Sci USA,1990,87∶6639-6643.6 Harai M,Suto Y,Kanoh M.

我们曾经介绍过一种荧光R显带的方法［10］，在此基础上建立了一种在显带染色体上直接观察杂交信号的新的定位方法：在细胞培养时，同时加入BUdR和Hoechst33258，PI染色后即呈现清晰的荧光R带带型，用彩色和全色黑白胶卷照相均可得到满意的结果，尤其适用于国内具有一般条件的实验室。

MATERIALS AND METHODS: Using Clinical Laboratory Standard Institute method, antifungal activity of fluconazole was determined alone and in combination with 16 chemosensitizers that included verapamil, reserpine, quinine, quinidine, gemfibrozil, lansoprazole, tamoxifen, diltiazem, desipramine, nicardipine, cyclosporine, chlorpromazine, prochlorperazine, promethazine, thioridazine, and trifluoperazine.

材料与方法：采用临床实验室标准制定的方法，氟康唑抗真菌活性的测定分为单独用氟康唑和氟康唑结合异博定、利血平、喹宁、喹纳定、吉非罗奇、地尔硫卓……等的测定。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

Methods By using the laboratory cytogenetic tests to investigate the radial-stricken workers.

方法采用实验室细胞遗传学的方法对有害作业人员进行调查。

objective to explore environment risk factors of metabolic syndrome.methods a cross-sectional population survey with questionnaires investigation,checkup and laboratory measurement for metabolic syndrome was performed among 2026 teachers,and the logistic regression was used to analyze the risk factors of ms.results the education,milk intakes,fish and aquatic products intakes,body exercise and drinking tea were different significantly between male and female individuals.univariate unconditional logistic regression analysis showed that education,body exercises,fish and aquatic products intakes and drinking tea were benefited to the ms,but the age,sucrose intakes were the risk factors to the ms.the multivariate logistic stepwise regression analysis showed that compared with individuals with no drinking milk,or no fish and aquatic product intake,or no drinking tea,or no sucrose intakes,the milk intakes 250-1500 g/week(or=0.731,95%ci:0.542-0.987),the fish and aquatic product intake with 250-1000 g/week(or=0.720,95%ci: 0.541-0.959),or sucrose intakes 250 g/month(or=0.446,95%ci:0.255-0.779),drinking tea forepassed(or=0.635,95%ci:0.458-0.883),and current (or=2.084,95%ci:1.390-3.125) had different levels of risk on ms.conclusion the age and sucrose intakes are risk factors,and the milk intakes,aquatic products and drinking tea benefits for ms.

目的 了解中小学教师代谢综合征发病及其影响因素。方法采用横断面调查方法，随机抽取芜湖市中小学教师2 026名，进行问卷调查、体格检查和实验室检测，并运用logistic回归分析代谢综合征影响因素。结果不同性别的中小学教师在受教育程度、牛奶摄入、水产品摄入和饮茶习惯等方面的差异有统计学意义；其中受教育程度、体育锻炼、水产品摄入和饮茶是保护因素，而年龄、工作紧张程度、糖的摄入可能是代谢综合征的危险因素。logistic逐步回归分析结果提示，牛奶摄入、水产品摄入和饮茶是代谢综合征的保护因素，而年龄和糖摄入是代谢综合征的危险因素，其中牛奶摄入在250～1500 g/周(or=0.731，95% ci=0.542～0.987)，水产品摄入在250～1000 g/周(or=0.720，95% ci=0.541～0.959)以及以前饮茶（or=0.446，95% ci=0.255～0.779和现在正在饮茶(or=0.635，95% ci=0.458～0.883)，对代谢综合征的保护作用明显，而糖摄入250 g/月时代谢综合征的患病的危险是不食用食糖的2倍(or=2.084，95% ci=1.390～3.125)。结论年龄和糖摄入是代谢综合征患病的危险因素，而牛奶、水产品和饮茶是代谢综合征的保护因素。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。