实验室方法

The preserve method of micro whole-blood samples has also be put into practice in disease area of endemic arsenism.

随后，在本实验室已经建立的微量末梢血样SCGE分析方法的基础上，探索了微量血液样品的保存方法，并对此方法在地方性砷中毒病区进行了实践。

Quantitative germicidal test method and chemical measuring method were used to carry out laboratory observation.

方法采用定量杀菌试验方法和化学测定方法在实验室进行了观察。

The final evaluation of the test was made at three UK laboratories and showed that the test can achieve in five hours what current microbiologic methods take two days to deliver.

对该检测方法的最终评估正在3所英国实验室中进行，显示该检测方法能够在5小时之内完成，而目前使用的微生物学检测方法需要2天才能够得到结果。

For Os separation, solvent extraction (Br_2 or CCl_4) and distillation are two mainly effective techniques, and microdistillation is used to further purify Os.

Os的分离纯化方法有蒸馏法和Br2或CCl4萃取法以及微蒸馏法，它们是分离和纯化Os的有效方法，也是目前国内外大多数实验室所采用的方法。

This non-specific antigen detects reagin , an IgM or IgG immunoglobulin present in the plasma or serum of patients with syphilis and occasionally present in the serum or plasma of individuals with other acute or chronic conditions. If reagin is present it binds to the cardiolipin antigen resulting in flocculation.

对个别病人应用同一种试验方法做连续的血清学检测，最好在同一个实验室进行，VDRL和RPR都是同样有效的方法，但是，由于RPR的滴度常常比VDRL的滴度略微偏高，两种方法的定量结果不能直接比较。

This paper gives several methods to validate these new methods by analyzing images of stage micrometer, metallurgical sample, and images that consisting of definite area in geometric graph, Several kinds of validation methods including of traceability of a quantity value, printing microstucture graph, comparing microstucture photo with standard photos, length meansurement, and area meansurement are discussed in this paper.

通过分析显微镜刻度尺图像、金相样品图像，及给定特定图形且已知面积的图像，依据ISO/IEC 17025检测和校准实验室能力的通用要求，并结合金相检验标准，阐述了金相显微镜利用新方法的方法确认，其中包括量值溯源、金相组织输出、图谱比较、长度类测量和面积类测量的确认方法。

We employed PIXE with high precision for analysis of trace element to study the relationship between the Neolithic pottery unearthed from Maliutuo, Maliuwan and Suheping sites in Sanxia reservoir area.

为了研究多种测量方法间的可比性，首次在国内对比了PIXE和ICP-AES两种方法的分析精度，结果表明本实验室使用的PIXE技术有足够的精度，可以和ICP-AES比拟，两种方法间具有互通性和互补性。

Methods Mosquitoes were collected by light-traps and human landing catches in villages;sporozoites were detected in the salivary glands under microscopy in the field sites and circumsporozoite proteins from mosquito was tested by ELISA.

方法村内采取诱蚊灯和人工诱捕方法捕蚊；现场采取显微镜解剖蚊虫的方法观察唾腺子孢子，实验室采用ELISA技术检测蚊虫体内环子孢子蛋白。

The method of metrological traceability of values for catalytic concentration of enzymes is now widely recognized as the combination of reference materials, reference measurement procedures and network of reference laboratories. Reference materials that are value-assigned using an internationally agreed upon protocol play an important role in this system and now reference materials or calibrators have come to our attention in our country.

随着人们对酶标准化工作和计量学溯源体系的深入认识，参考方法、参考物质和参考实验室网络的结合成为国内外普遍认可的实现酶活性测定标准化的重要途径，而使用公认的酶校准物作为载体，将测定结果的数值在参考方法和常规方法间进行准确性的传递是目前酶标准化工作的关键环节之一。

The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。