实验室方法

Using the methodology of radical cyclizations of α-acylamino radicals with acylsilanes to construct pyrrolizidine, indolizidine and quinolizidine skeletons, we were able to synthesize-Lentiginosine,-Epiquinamide,-Swainsonine and other analogous alkaloids.

本论文是延续实验室利用α-醯胺基碳自由基与矽基酮的环化反应来合成吡咯叻咭啶、吲哚叻咭啶和喹叺叻咭啶等骨架的方法，进而企图合成出-Lentiginosine、-Epiquinamide、-Swainsonine及其它类似的生物碱。

The apomictic monosomic addition line of Beta corolliflora, designated as M14(VV+IC, 2n=18+1), was obtained from remote hybridization of B. vulgaris L.

本实验室的郭德栋教授通过远缘杂交方法获得了甜菜无融合生殖单体附加系M14品系(VV+1C，2n=18+1)，以下简称甜菜M14品系。

In order to obtain pure axenic spores for further experiments, four different commonly used surface sterilization methods were compared.

采用不同的表面消毒方法和不同的培养基质，探索了促使孢子萌发的一些主要外部环境条件，以便在现有实验室条件下更多、更快地获得大量能够萌发的纯净孢子。

Through field,potand incubation experiments,the effect of Azolla on CH4 and N2O emissionsfrom rice field and its mechanism were systematically studied.

本文采用田间试验、盆栽试验及实验室模拟相结合的研究方法，首次系统地研究了稻田养萍对CH4和N2O排放的影响及其机理，得到下述主要结果

Traditional phenotype classification,chemotaxonology and other ordinary bacteria taxonology were used to study the antagonistic bacterium preserved by the agricultural antibiotic laboratory of Chengdu Institute of Biology, Chinese Academy of Sciences.Its morphological,cultural physiological,biochemical characteristics and chemotaxonomy were studied.The antagonistic bacterium is bacilliform,and its cell diameter is less than 1 micron;it is Gram positive;it.can form spore.

采用传统的表型分类、化学分类等一般细菌常用鉴定方法，对中科院成都生物所农抗组实验室保存的一株小麦赤霉菌拮抗菌株进行形态特征、培养特征、生理生化特征等水平的鉴定，该细菌的细胞呈杆状，细胞直径小于1微米，革兰氏染色阳性，能形成芽孢，属于芽孢杆菌属。

Nuclear chromatin has a high nudeic acid content and therefore is attracted to the blue, more basic dye (i.e., it is basophilic).

苏木精和伊红染色是实验室最常用的方法。苏木精染成蓝色或绛红色，伊红染成红色。

A new method is used for the rapid determination of SOn^2- in laboratory waste water by benzidine hydrochloride separate and acid-base titration.

中文摘要：提出了利用盐酸联苯胺沉淀分离，酸碱滴定法快速测定实验室废水中SO4^2-含量的新方法。

Bromate, perbromate, hypochlorite, perchlorate, ferrate,bismuthate, hydrogen peroxide, ozone, oxygen,hydroxyl radical, vanadate, ultraviolet light, bombcolorimetry, combinations of several oxidants andelectrochemical techniques. These approaches have notbeen suitable due to difficulties in reagent preparation,reagent stability, photosensitivity, low oxidationpotential, poor oxidation efficiency, expense, and userprotocols that proved too complex.

哈希实验室就试验过高锰酸盐，铈盐，过硫酸盐，过碘酸盐，碘酸盐，高铁酸盐，溴酸盐，过溴酸盐，过氧化氢，臭氧，氧，羟基，钒酸盐，紫外光，氧化弹，比色计，数种氧化剂与电化学技术的结合，这些方法已不适用，由于试剂难于制备，试剂稳定性，光敏感，低氧化电位，低氧化率，昂贵，用户证实太复杂方面的问题。

In the experiments reported in Nature, Jacobsen's group collaborated with computational biologists Matteo Pellegrini and Shawn Cokus, as well as researchers from the biotechnology companies Illumina and New England BioLabs to create new molecular biology methods, algorithms, and software to analyze bisulphite sequences.

在《自然》杂志里如是报道，雅克布森的团队与计算生物学家Matteo Pellegrini ＆ Shawn Cokus合作，还有来自Illumina生物技术公司和新英格兰生物实验室的研究者们，建立了新的分子生物学方法、算法和软件，用于解析亚硫酸氢盐测序。

Methods In 28 patients of ED caused by the posterior urethral injury complicated with pelvic fracture, diagnosis and classification were carried out by means of detail history, physical examination, laboratory examination, international index of erectile function, night penile test, papaverine test, penis Doppler ultersound, dynamicavernorography, bulbocavernosus reflex and urodynamics test.

通过病史、查体、实验室检查，国际勃起功能指数，NPT、罂粟碱试验、阴茎Doppler声、动态阴茎海绵体造影、球海绵体肌反射和尿动力学检查等方法，对骨盆骨折并后尿道损伤引起的阴茎勃起功能障碍28例患者进行诊断、分类，对不同类型的ED患者进行相应治疗。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。