实验室方法

This chapter is not intended for retroactive application to already successfully established laboratory procedures.

此章节并不旨在对已经成功建立的实验室方法进行回顾性运用。

Objectives: To establish a laboratory method of detecting p47 antigen of Candida albicans in serum and to evaluate its clinical application.

目的： 建立检测血清中白色念珠菌相对分子质量47 000(p47)抗原的实验室方法，并探讨其临床应用价值。

It contains forset tree genome database information, including information on genetic maps, PCR primers, DNA sequences, images, laboratory protocols, genome software, and instructions for submitting data to the TreeGenes database.

该网站提供了森林植物的基因组数据库的信息，包括基因图谱的构建，PCR引物，DNA序列，图象，实验室方法，基因组软件，以及向树木基因组数据库提交数据的指导。

Juergen Pipper says the device can process complex tasks in a way similar to a traditional biological laboratory.

这个科学家说此设备可以进行更加复杂的任务，其进行方法类似于传统的生物实验室方法。

PART V : LABORATORY METHODS IN ANECHOIC AND SEMI-ANECHOIC ROOMS.

第5部分：吸音厅和半吸音厅实验室方法

Development of different SiC materials manufacturing by industrial and laboratory method are reviewed.

本文综述了工业生产及实验室方法合成各种碳化硅材料的制备技术。

Laboratory method,in-situ testing method and acoustic remote method ,have been developed in practice.

在实践应用中有实验室方法、现场测量方法和海底声学遥测方法，并研制出大量的仪器。

Titration is a common laboratory method of quantitative chemical analysis that is used to determine the unknown concentration of a known reactant.

滴定是定量化学分析中常见的实验室方法，用于测定已知反应物的未知浓度。

Rresults from those studies can be summariazed as follow:(1) A preliminary study no correlationship between the UV-VIS reflection spectrum of tomato fruit surface and the lycopene amount of tomato fruit was undertaken. Based on observation from this study, a damage-free and in-situ detection technique of lycopene amount from tomato was developed with UV-VIS reflection spectroscopy;(2) The geometrical isomers of lycopene were separated successfully on C30-HPLC-PDA-ELSD. The absorption coefficients of Z-isomers were consequently dedermined. The geometrical isomer composition of lycopene could therefore be assessed. Those efforts formed a solid base to identify the natural product from synthetic compound of lycopene;(3) Lycopene resource from the fruit of Autumn oliver was proved to be available for industrial application. An extraction methos of lycopene from the fruit of Autumn oliver by supercritical carbon dioxide was developed at laboratory scale;(4) A method to extract lycopene by supercritical liquid with improved efficiency was developed; Data from this investigation suggested that a proper raw material pre-extraction process was important for a better extraction efficiency;(5) Variation in the geometrical isomer composition of lycopene during its metabolish in rat was assessed. A large amount of Z-isomers were observed. This variation took place in serum;(6) The absorption rate and accumulation in serum of natural lycopene in rat were assessed;(7) Ability to quench singlet oxygen by different geometrical isomers of lycopene was compared;(8) It was detected that whether natural lycopene had functions to regule blood-lipids and LDL antioxidation;(9) A C31 degradation piece of lycopene was found in the prostate gland of rat.

研究结果包括：[1]应用UV-VIS反射光谱法建立了番茄果实中番茄红素含量的无损伤、现场检测技术；[2]应用C30-HPLC-PDA-ELSD技术实现了对番茄红素几何异构体的分离，测定了不同顺式异构体的吸光系数，建立了天然番茄红素几何异构体组成的检测方法，为区分和鉴定人工合成品和天然产物奠定了基础；[3]证实了秋橄榄果实中的番茄红素资源具有工业开发价值，并建立了超临界CO_2萃取的实验室方法；[4]探索了提高番茄红素超临界流体萃取效率的方法，证明了物料前处理对提高萃取效率起了极其重要的作用；[5]观察了番茄红素在大鼠体内几何异构体组成的变化情况，发现了大量顺式异构体的存在，并证实了几何异构体组成在血清中发生了显著变化；[6]测定了天然番茄红素在大鼠体内的吸收率和在大鼠血清中的动态积累规律；[7]比较了不同番茄红素几何异构体淬灭单线态氧的能力；[8]检测了天然番茄红素是否具有调节血脂作用和抗低密度脂蛋白氧化的功能；[9]首次发现大鼠前列腺内存在番茄红素C31降解片段，并证实其仍具有淬灭单线态氧的能力。

The team then used modern DNA lab methods to compare mitochondrial control region sequences from Norfolk Island fossils with those from a wider sample of modern bird throughout the boobies' range.

该小组随后运用现代DNA实验室方法，在线粒体控制区序列上，将诺福克岛的化石与更多鲣鸟科里的现代品种样本进行了比对。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。