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GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株,经筛选后倒置荧光显微镜观察细胞形态及荧光状态,挑取荧光最强的克隆,命名为ES-GFP细胞,进一步扩增培养,观察细胞生长及荧光表达情况,行细胞分化全能性鉴定包括碱性磷酸酶(alkine Phosphtase,AKP)染色、体外胚胎体(embryonic body,EB)形成实验及裸鼠体内成瘤实验。2。

Methods Chicken embryo allantois membrane was used in the experiment. Breast cancer cells were inoculated on the superior surface of CAM sac, then the vascular tissues were collected in lower sac after incubation.

通过鸡胚尿囊绒膜实验和骨条黏附实验确定BSP的高稳定表达对乳腺癌细胞血管侵袭能力及骨条黏附能力的影响。

The batch reactor often used to investigate the kinetics of crystal growth on seeded precipitation of sodium aluminate solution .

在铝酸钠溶液的种分过程中,往往利用间歇式反应器来研究晶体的长大动力学,由于受实验过程条件的变化及成核和小粒子附聚的影响,实验结果往往不令人满意。

Uses this set of test installation system, has conducted the experimental study to the aluminite powder and the cyclotrimethyl dust's detonation characteristic.

利用这套实验装置系统,对铝粉和黑索金粉尘的爆炸特性进行了实验研究。

In this paper, the flame retardation of Amatol, TNT, explosive B, RDX, HMX and plastic bonding explosives manufactured from them are discussed. Several explosives with satisfied flame retardancy and explosion performances were obtained for the first time. Alloy bath method, improved hot wire test and flame test are employed to evaluate fire retardancy of fire retarded explosives.

本文研究了AMATOL炸药,TNT,B炸药,RDX和HMX也涉及到了一部分塑料粘结炸药的阻燃处理,首次制得了具有较好阻燃性能而且能基本保持炸药原有爆炸性能的阻燃炸药,首次采用了合金浴升温实验,改进了热丝实验和火焰测试方法并用以上方法来评价阻燃炸药的阻燃性能。

The mice were then conducted to take water-maze test and y-maze test to evaluate the effects of ammonium chloride on learning and memory function.

通过水迷宫实验和y型迷宫实验观察氯化铵对小鼠学习记忆功能的影响。

The study indicated that the solid dispersion have been changed into mono phase system from double phase system, and the docetaxel present in the carriers with amorphism or molecular, which insured the drug could be dissolved quickly in gastrointestinal tract.

实验表明,固体分散体从两相体系转变为单相体系,且多烯紫杉醇在载体材料中以无定形或分子形态存在,保证了多烯紫杉醇在胃肠道中的快速溶出,达到了本实验的预期目的。

Deformation experiment of amphibole shows that ,under conditions of pressure that is similar to the depth at which crustal low velocity layer of the Qinghai Xizang plateau is buried (20 30 km), and temperature heated to 800℃and 1000℃,amphibolite undergoes plastic deformation and enters steady creep strain states.

在相当于青海—西藏高原地壳低速层埋藏深度(20 ~30 km)的压力条件下进行了角闪岩的变形实验实验表明,当加热到800℃、1000 ℃时,角闪岩出现塑性变形,并进入稳定蠕动应变状态。

We have performed partial melting experiments on a massive amphibolite sample under 1.5 GPa and 950 ℃ for 101 hours: the sample was collected from Archaean terrain in the northern margin of the North China craton.

在1.5 GPa,950 ℃,恒温101 h条件下对华北北缘太古宙地体中的斜长角闪岩块状样品进行了脱水部分熔融实验实验产物组合为Hb+Cpx+Gt+Gl,获得的熔体为花岗闪长质成分。

The enzyme-inactivated and fat-skimmed material and 80% ethanol as the solvent were used in the extraction of amygdalin. The optimum extraction techniques were determined by the orthogonal experiment which was based on the single-factor experiment.

原料经灭酶、脱脂后,确定以80%乙醇溶液为溶剂来提取苦杏仁苷,通过单因素实验确定正交实验方案,进而得出较佳的提取工艺。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。