实验

The aim of this study was to investigate the expression of mPGES-2 protein in mouse oviduct during the early pregnancy, pseudopregnancy, and estrous cycle by immunohistochemistry and Western blot. The regulation of mPGES-2 protein in mouse oviducts was also studied under delayed implantation, steroid hormonal treatments and oviductal ligation.

本实验主要利用早期妊娠、假孕、输卵管结扎、发情周期、激素调节以及延迟着床等实验模型，结合免疫组织化学和Western blot 的实验方法，详细研究了mPGES-2 蛋白在小鼠输卵管中的表达与调节。mPGES-2 蛋白主要定位于不同时期的伞部和壶腹部的上皮纤毛细胞中，在伞部和壶腹部肌层以及峡部和宫管连接处的上皮和肌层中未检测到mPGES-2 蛋白的表达。

Methamidophos affected the mean ratio of ovigerous to non-ovigerous females and the mean mictic rate significantly but had less effect on the total resting egg production, while 17β-estradiol was in adverse.

甲胺磷和17β-雌二醇对实验期间轮虫的平均种群增长率均有显著影响，但对轮虫种群中的混交雌体受精率的平均值无显著影响；甲胺磷对实验期间轮虫种群中的携卵雌体数／非携卵雌体数和混交雌体百分率的平均值均有显著影响，而17β-雌二醇则无显著影响；甲胺磷对实验期间轮虫的休眠卵总产量无显著影响，而17β-雌二醇的影响较显著。

Methods 80 patients in need of palatoplasty were randomly divided into four groups: NO.1 Experiment Group, NO.2 Experiment Group, NO.3 Experirnent Group and the control group.

选择行腭裂修复术病人 80例，随机分为实验Ⅰ组、实验Ⅱ组、实验Ⅲ组和对照组，每组 2 0例，分别给予 3%双氧水及 0 。1%洗必泰溶液含漱；0 。1%洗必泰溶液含漱；生理盐水含漱；常规口腔护理。结果四种方法均能使口腔内细菌菌落数明显降低，且护理前后有显著差异(P 。0 0 1)。

The periostea of both experimental and control side of the mandibular ramus were taken and prepared, 2 of each 5 rabbits in a group were prepared for HE stain detection and 3 for proliferating cell nuclear antigen immunohistochemical detection.Results:1, The newly formed bone was detected on the lateral aspect of mandibular ramus after periosteal distraction. The bone was shaped like a hill. It looked very low and was full of holes at postoperative day 28. With the time of consolidation period lengthened, the newly formed bone matured gradually. X-ray examination showed the new bone shaped like a hill. The average values of new bone height at postoperative days 28,35,42 and 56 were 1.86 + 0.15mm, 2.29 + 0.29mm,3.19 + 0.13mm and 4.70 + 0.45mm. Histological examination of both HE stain and picricacid-fuchsin stain showed the increase in the number of osteoblasts and the change in the orientation of collagen fibers and bone trabecula. There were no significant differences between newly formed bone and original bone on the lateral aspect of mandibular ramus at postoperative day 56 histologically.2 Compared with the control side, the distracted periostea proliferated obviously under the microscope, and the number of periostealcells increased with satiation of cellular nuclear per unit area. The images of PCNA immunohistochemical stain of periosteum showed that the experimental periosteum proliferated obviously after distraction compared with the control side.

结果：骨膜牵张成骨的实验研究南京医科大学硕{学位论文l、骨膜牵张后，可见下领升支外侧的骨皮质上有新骨形成，新骨呈山峰状凸起，术后第28天的新生骨较低平，多孔隙，随着固定时间的延长，新骨逐渐成熟；下领升支前后向切线位X线投照显示新骨呈山峰样隆起；经测量，术后第28、35、42和56天组平均新生骨厚度分别为x.86士0.15mm、2.29士0.29mm、3.19士0.13mm和4.70 土0.45mm；脱钙骨组织的HE染色和不脱钙骨组织的苦味酸一品红染色的组织学观察均显示了新生骨在成骨细胞数量上的增长，以及胶原纤维和骨小梁排列方向上的变化，术后第56天的新生骨在组织学上与原升支骨组织已无明显区别。2、HE染色显示，与对照组相比较，实验侧骨膜增生明显，细胞间排列紧密，单位面积内骨膜细胞数增多，细胞核饱满；骨膜PCNA 免疫组化染色显示，与对照侧相比较，实验侧骨膜在牵张后出现了明显的增生迹象，PCNA阳性细胞分布紧密，单位面积内阳性细胞数较对照组多，靠近骨表面的骨膜中的阳性细胞数更多而且分布更为紧密。

Condition filter experiments for determination of the iron by phenanthroline spectrophotometry in the genral textbook are single factor experiments, and there are some problems in the experimental design.

普通教材中邻二氮菲分光光度法测定铁条件选择实验都是单因素条件实验，不够全面，实验设计中也存在一些问通。

With Platycodon root as experiment material, on the base of single factor experiments (extraction time, extraction temperature and so on), then through the experimental design of response surface methology, the conditions of extraction are studied.

以桔梗为实验材料，在提取温度、提取时间、料液比等单因素实验的基础上，利用响应曲面实验设计法对桔梗皂甙提取条件进行研究，并通过回归方程和响应曲面，得到了最佳提取工艺。

With Platycodon root as experiment material and Platycodon extract,sucrose,citric acid and β-cycloheptaamylose as experiment factors,on the base of Response Surface Methodology Analysis,researches on optimal prescription of Platycodon health protection beverage were conducted.

以桔梗为实验材料，以桔梗提取物、β-环状糊精、蔗糖和柠檬酸为实验因素，利用响应曲面法进行实验设计，对桔梗保健饮料的最佳配方进行研究，并对最佳配方进行感官评价验证，理化指标检测和微生物学检测。

On the control strategy of liquid temperature, devoleping precompensation PIDstrategy was adopted based on the previous works. Considering the whole system, weadapt Linux OS that is steady than the MCU. It can complete the experiment by manypeople and many PC through the net. The system used Linux operation system based onthe ARM9 as the real-time kernel and the nether application software developmentenvironment. So it\'s easy to extend and maintain the system.

在实验温度控制上，详细分析了多种控制方案的优缺点，在改进前人的控制算法基础上选取了改进的分阶段预补偿PID控制技术，实现了温度的精确控制；从整个系统的需求出发，采用了比单片机更稳定的Linux操作系统，通过网络使得多人多机能够参与到实验中，更好的完成了杂交实验，实现了全系统的优化；在软件开发时选用了基于ARM9的Linux操作系统作为实时内核和下位机软件开发环境，极大地方便了系统的扩展和维护。

In the second experiment, we would like to understand the relationship between sexual performance and sexual motivation. The partner preference test was conducted to measure sexual incentive motivation, and tyrosine hydroxylase immunohistochemistry was used to assay the dopaminergic neuron activity in the medial preoptic area.

在第二部份实验所探讨的是雄鼠的性动机与性表现两者之关系，本实验以EGb 761作为一实验工具，利用伙伴偏好试验测试雄鼠性诱因动机，并利用酪氨酸羟化酶(tyrosine hydroxylase， TH)免疫化学染色方法分析内侧视前区多巴胺神经元的活性。

The research of polymer/layered silicate nanocomposite was introduced detailedly, including the classify, property and the latest research advance. On the basis of consulting the document and the exploratory experiment, the experimental method which first creative preparing poly (acrylonitrile-vinyl acetate)/raontmorillonite nanocomposite by emulsion intercalation copolymerization was established; the structure and property of polyacrylonitrle different molecule weight with inorganic mineral super fine nanocomposite was reseached. The reaction principle of PMMA by photo-initution was explored. The mixture principle with productor condition, property of PE/Clay nanocomposite milt insert was seal prived. By PS/Clay nanocomposite munufacture, the monomer ins-polymerization and polymerial solution was proved.

在查阅文献和探索性实验的基础上，确定采用乳液原位插层聚合方法首先创制了聚/蒙脱土纳米复合材料，利用正交实验得出了最佳实验条件，通过X射线衍射和红外光谱对纳米复合材料的结构进行表征，研究了复合材料的溶解性能，并测定了其溶液的绝对粘度；研究了不同相对分子质量的聚丙烯腈与不同的无机矿物超细粉复合材料的结构与性能；探讨了在蒙脱土中光引发聚甲基丙烯酸甲酯的反应机理；通过聚乙烯与粘土的纳米复合材料制备，探讨了热塑性树脂熔融插层复合机理与最佳工艺条件和复合材料性能关系；通过聚苯乙烯与蒙脱土纳米复合材料制备，验证了单体原位聚合插层和聚合物溶液插层机理。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。